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| DNA Polymorphisms of 5´-Flanking Region of Insulin-Like Growth Factor 1 Gene and Their Association with Reproduction Traits in Goats |
| WANG Ping-qing, TAN Ying, ZHANG Bao-yun, CHU Ming-xing, DENG La-mei, FAN Qi , LIUChong-xu |
1.College of Bioengineering, Chongqing University
2.Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation, Ministry of Agriculture/Institute of Animal Science,Chinese Academy of Agricultural Sciences |
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摘要 Research on the identity of genes and their relationship with traits of economic importance in farm animals could assist in the selection of livestock. In this study, the polymorphisms of insulin-like growth factor 1 (IGF1) gene in 561 goats of ten breeds were detected by polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP) and their association with litter size and birth weight in three breeds were investigated. The effects of IGF1 polymorphisms on the breeding value for litter size and birth weight were examined using least square methods. Two deletions (CA) were detected in the microsatellite and two mutations (A1637G, T1640C) were found in 5´-flanking regulatory region. No significant association between the polymorphisms in 5´-flanking region of IGF1 and birth weight was found in the three breeds of goats. In Gulin Ma goats, two polymorphisms were found to affect litter size traits. In Chuandong White goats and Guizhou White goats, no significant difference (P<0.05) in litter size between goats carrying different genotypes was observed. Further evaluation and confirmation studies in more goat populations with larger sample sizes are necessary.
Abstract Research on the identity of genes and their relationship with traits of economic importance in farm animals could assist in the selection of livestock. In this study, the polymorphisms of insulin-like growth factor 1 (IGF1) gene in 561 goats of ten breeds were detected by polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP) and their association with litter size and birth weight in three breeds were investigated. The effects of IGF1 polymorphisms on the breeding value for litter size and birth weight were examined using least square methods. Two deletions (CA) were detected in the microsatellite and two mutations (A1637G, T1640C) were found in 5´-flanking regulatory region. No significant association between the polymorphisms in 5´-flanking region of IGF1 and birth weight was found in the three breeds of goats. In Gulin Ma goats, two polymorphisms were found to affect litter size traits. In Chuandong White goats and Guizhou White goats, no significant difference (P<0.05) in litter size between goats carrying different genotypes was observed. Further evaluation and confirmation studies in more goat populations with larger sample sizes are necessary.
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Received: 03 August 2010
Accepted:
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| Fund: This work was supported by the earmarked fund for Modern Agro-Industry Technology Research System of China (nycytx-39) and the Natural Science Foundation Project of CQ CSTC, Chongqing City, China (CSTC, 2009BA1066). |
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Corresponding Authors:
Correspondence WANG Ping-qing, Professor, Tel: +86-23-65112753, E-mail: wang_pq@21cn.com
E-mail: wang_pq@21cn.com
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| About author: WANG Ping-qing, Professor, Tel: +86-23-65112753, E-mail: wang_pq@21cn.com |
Cite this article:
WANG Ping-qing, TAN Ying, ZHANG Bao-yun, CHU Ming-xing, DENG La-mei, FAN Qi , LIUChong-xu .
2011.
DNA Polymorphisms of 5´-Flanking Region of Insulin-Like Growth Factor 1 Gene and Their Association with Reproduction Traits in Goats . Journal of Integrative Agriculture, 10(10): 1609-1617.
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