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| Erythropoietin Receptor Gene (EPOR) Polymorphisms are Associated with Sow Litter Sizes |
| ZHANG Long-chao, WANG Li-gang, LI Yong, YAN Hua, ZHAO Ke-bin and WANG Li-xian |
| Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China |
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摘要 The erythropoietin receptor (EPOR) has shown to play an important role in fetal survival by promoting the maturation of red blood cells in many studies of uterine capacity and litter size in swine. In this study, we screened the porcine EPOR gene for mutations and identified five single nucleotide polymorphisms (SNPs): g.705G>T in intron 1, g.2 373C>T in intron 4, and g.2 882C>T, g.3 035A>G, and g.3 132A>T in intron 6. We then genotyped 247 Beijing Black (BB) sows and compared the polymorphism data with the litter sizes of 1 375 parities among the sows. At first parity, there was no association of g.2 882C>T and g.3 132A>T with litter sizes. However, the CT sows in g.2 882C>T had 2.13 higher total number born (TNB) (PT had the highest litter size when compared to the two homozygotes for the later parities (PG SNP,for the later parities, the TNB of the sows with the GG genotype was 3.81 higher (PT SNP was associated with a greater litter size at both the first parity (PT SNP was significantly more common in the more prolific Chinese breeds. These results indicated that the EPOR could be an important candidate gene for litter size and g.705G>T can serve as a useful genetic marker for improving litter size in both first and later parities in swine.
Abstract The erythropoietin receptor (EPOR) has shown to play an important role in fetal survival by promoting the maturation of red blood cells in many studies of uterine capacity and litter size in swine. In this study, we screened the porcine EPOR gene for mutations and identified five single nucleotide polymorphisms (SNPs): g.705G>T in intron 1, g.2 373C>T in intron 4, and g.2 882C>T, g.3 035A>G, and g.3 132A>T in intron 6. We then genotyped 247 Beijing Black (BB) sows and compared the polymorphism data with the litter sizes of 1 375 parities among the sows. At first parity, there was no association of g.2 882C>T and g.3 132A>T with litter sizes. However, the CT sows in g.2 882C>T had 2.13 higher total number born (TNB) (P<0.01) and 1.81 higher number born alive (NBA) (P<0.01) than the CC sows and the heterozygous sows in g.3 132A>T had the highest litter size when compared to the two homozygotes for the later parities (P<0.05). In the g.3 035A>G SNP, for the later parities, the TNB of the sows with the GG genotype was 3.81 higher (P<0.01) and the NBA was 2.75 higher (P<0.01) than that with the AA genotype but no difference at first parity. The G allele of the EPOR g.705G>T SNP was associated with a greater litter size at both the first parity (P<0.05) and later parities (P<0.01). Furthermore, we determined the allele frequencies for this SNP among five Chinese indigenous pig breeds (Erhualian, Laiwu Black, Meishan, Min, and Rongchang) and three western commercial pig breeds (Duroc, Landrace, and Large White). The G allele of the EPOR g.705G>T SNP was significantly more common in the more prolific Chinese breeds. These results indicated that the EPOR could be an important candidate gene for litter size and g.705G>T can serve as a useful genetic marker for improving litter size in both first and later parities in swine.
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Received: 10 June 2011
Online: 10 June 2011
Accepted:
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Corresponding Authors:
WANG Li-xian
E-mail: zhlchias@163.com;iaswlx@263.net
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| About author: ZHANG Long-chao, Assistant Professor, Ph D, E-mail: zhlchias@163.com; Correspondence WANG Li-xian, Professor, Tel/Fax: +86-10-62818771, E-mail: iaswlx@263.
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Cite this article:
ZHANG Long-chao, WANG Li-gang, LI Yong, YAN Hua, ZHAO Ke-bin and WANG Li-xian.
2011.
Erythropoietin Receptor Gene (EPOR) Polymorphisms are Associated with Sow Litter Sizes. Journal of Integrative Agriculture, 10(6): 931-937.
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| Bazer F W, Clawson A J, Robison O W, Ulberg L C. 1969a. Uterine capacity in gilts. Journal of Reproduction and Fertility, 18, 121-124. Bazer F W, Robison O W, Clawson A J, Ulberg L C. 1969b. Uterine capacity at two stages of gestation in gilts following embryo superinduction. Journal Animal Science, 29, 30-34. Bugge A, Siersbæk M, Madsen M S, Göndör A, Rougier C, Mandrup S. 2010. A novel intronic peroxisome proliferator activated receptor (PPAR) γ enhancer in the uncoupling protein (UCP) 3 gene as a regulator of both UCP2 and -3 expression in adipocytes. Journal of Biological Chemistry, 285, 17310-17317. Cheng P L. 1985. Livestock breeds of China FAO Animal Production and Health Paper. vol. 46. Agriculture and Consumer Protection Department, FAO. Cheng P L. 1986. Pig Breeds in China. Shanghai Scientific and Technical Publishers, Shanghai. (in Chinese) Chiba T, Ikawa Y, Todokoro K. 1991. GATA-1 transactivates erythropoietin receptor gene, and erythropoietin receptormediated signals enhance GATA-1 gene expression. Nucleic shed by Elsevier Ltd. Acids Research, 19, 3843-3848. Chin K, Oda N, Shen K, Noguchi C T. 1995. Regulation of transcription of the human erythropoietin receptor gene by proteins binding to GATA-1 and Sp1 motifs. Nucleic Acids Research, 23, 3041-3049. Christenson R K. 1993. Ovulation rate and embryonic survival in Chinese Meishan and white crossbred pigs. Journal Animal Science, 71, 3060-3066. Ghezzi P, Brines M. 2004. Erythropoietin as an antiapoptotic, tissue-protective cytokine. Cell Death and Differentiation, 11, S37-S44. Jelkmann W. 1992. Erythropoietin: structure, control of production, and function. Physiological Reviews, 72, 449- 489. Johnson R K, Zimmerman D R, Kittok R J. 1984. Selection for components of reproduction in swine. Livestock Production Science, 11, 541-557. Liboi E, Carroll M, D’Andrea A D, Mathey-Prevot B. 1993. Erythropoietin receptor signals both proliferation and erythroid-specific differentiation. Proceedings of the National Academy of Sciences of the USA, 90, 11351-11355. Longmore G D, Watowich S S, Hilton D J, Lodish H F. 1993.The erythropoietin receptor: its role in hematopoiesis and myeloproliferative diseases. The Journal of Cell Biology, 123, 1305-1308. Markljung E, Jiang L, Jaffe J D, Mikkelsen T S, Wallerman O, Larhammar M, Zhang X, Wang L, Saenz-Vash V, Gnirke A, et al. 2009. ZBED6, a novel transcription factor derived from a domesticated DNA transposon regulates IGF2 expression and muscle growth. PloS Biology, 7, e1000256. Mason I L. 1996. A World Dictionary of Livestock Breeds, Types and Varieties. 4th ed. CAB International, Wallingford. Miller S A, Dykes D D, Polesky H F. 1988. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research, 16, 1215. Miyake T, Kung C K, Goldwasser E. 1977. Purification of human erythropoietin. The Journal of Biological Chemistry, 252, 5558-5564. Moritz K M, Lim G B, Wintour E M. 1997. Developmental regulation of erythropoietin and erythropoiesis. American Journal of Physics, 273, R1829-R1844. Ohneda K, Yamamoto M. 2002. Roles of hematopoietic transcription factors GATA-1 and GATA-2 in the development of red blood cell lineage. Acta Haematologica, 108, 237-245. Onteru S K, Ross J W, Rothschild M F. 2009. The role of gene discovery, QTL analyses and gene expression in reproductive traits in the pig. Society of Reproduction and Fertility Supplement, 66, 87-102. Pearson P L, Klemcke H G, Christenson R K, Vallet J L. 1998. Uterine environment and breed effects on erythropoiesis and liver protein secretion in late embryonic and early fetal swine. Biology of Reproduction, 58, 911-918. Pearson P L, Smith T P L, Sonstegardt T S, Klemcke H G, Christenson R K, Vallet J L. 2000. Porcine erythropoietin receptor: molecular cloning and expression in embryonic and fetal liver. Domestic Animal Endocrinology, 19, 25-38. SAS Institute. 2001. SAS/STAT User’s Guide. Release 8.2. SAS Institute, Inc., Cary, NC. Strauss E C, Orkin S H. 1992. In vivo protein-DNA interactions at hypersensitive site 3 of the human b-globin locus control region. Proceedings of the National Academy of Sciences of the USA, 89, 5809-5813. Vallet J L, Freking B A, Leymaster K A, Christenson R K. 2005. Allelic variation in the erythropoietin receptor gene is associated with uterine capacity and litter size in swine. Animal Genetics, 36, 97-103. Vallet J L, Klemcke H G, Christenson R K, Pearson P L. 2003. The effect of breed and intrauterine crowding on fetal erythropoiesis on day 35 of gestation in swine. Journal of Animal Science, 81, 2352-2356. Vallet J L. 2000. Fetal erythropoiesis and other factors which influence uterine capacity in swine. Journal of Applied Animal Research, 17, 1-26. Wang J Y, Wu Y, Zhang D L, Wang H Z, Guo J F, Zhang Y, Wang C. 2008. Study on the polymorphisms of reproductive genes and their effects on litter size in Laiwu Black Pigs. Acta Agriculturae Boreali-occidentalis Sinica, 17, 7-10. (in Chinese) Youssoufian H, Longmore G, Neumann D, Yoshimura A, Lodish H F. 1993. Structure, function, and activation of the erythropoietin receptor. Blood, 81, 2223-2236. Youssoufian H. 1994. Further characterization of cis-acting regulatory sequences in the genomic locus of the murine erythropoietin receptor: evidence for stage-specific regulation. Blood, 83, 1428-1435. |
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