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Journal of Integrative Agriculture  2022, Vol. 21 Issue (4): 1028-1043    DOI: 10.1016/S2095-3119(21)63725-5
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The use of widely targeted metabolite profiling to reveal the senescence changes in postharvest ‘Red Globe’ (Vitis vinifera) grape berries
XU Teng-fei1*, YANG Xin2*, ZHANG Meng2, GUO Shui-huan2, FU Wen-jing2, ZHOU Bi-jiang2, LIU Yu-jia2, MA Hai-jun3, FANG Yu-lin2, YANG Gang4, MENG Jiang-fei2
1 College of Horticulture, Northwest A&F University, Yangling 712100, P.R.China
2 College of Enology, Northwest A&F University, Yangling 712100, P.R.China
3 College of Biological Science and Engineering, North Minzu University, Yinchuan 750021, P.R.China
4 Shenzhen Camartina Wine Co., Ltd., Shenzhen 518031, P.R.China
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Abstract  Changes in the metabolites of table grapes (Vitis vinifera) reportedly occur during postharvest senescence.  The aim of this study was to determine the metabolomic differences in postharvest table grapes (‘Red Globe’) after being subjected to different senescence periods.  To this end, we used widely targeted metabolomics based on ultra-performance liquid chromatography and tandem mass spectrometry.  A total of 135 differential metabolites were identified.  During postharvest senescence, the levels of most differential flavonoids (e.g., pelargonidin 3-O-glucoside, quercetin-3-O-glucoside, and cyanidin 3-O-glucoside) and L-aspartic acid decreased, while the levels of phenolic acids (e.g., trans-4-hydroxycinnamic acid methyl ester) and pantothenol increased.  During early and late senescence, the levels of most differential lipids, especially LysoPC, as well as those of nucleotides and their derivatives, such as uridine, decreased and increased, respectively.  Collectively, the findings of this study provide fundamental insights into the reasonable control of table grape fruit postharvest senescence and lay a solid foundation for further research.
Keywords:  table grape       berry        postharvest senescence        widely targeted metabolites  
Received: 16 December 2020   Accepted: 10 May 2021
Fund: This work was supported by the Natural Science Foundation of China (31801833 and 31801811), the Innovation Capability Support Programs of Shaanxi Province, China (2020KJXX-035), the China Postdoctoral Science Foundation (2019M653771 and 2019T120953), the Fundamental Research Funds for the Central Universities of China (2452019016), and the China Agriculture Research System of MOF and MARA (CARS-29-zp-6).

About author:  XU Teng-fei, E-mail:; Correspondence YANG Gang, E-mail:; MENG Jiang-fei, E-mail: * These authors contributed equally to this study.

Cite this article: 

XU Teng-fei, YANG Xin, ZHANG Meng, GUO Shui-huan, FU Wen-jing, ZHOU Bi-jiang, LIU Yu-jia, MA Hai-jun, FANG Yu-lin, YANG Gang, MENG Jiang-fei. 2022. The use of widely targeted metabolite profiling to reveal the senescence changes in postharvest ‘Red Globe’ (Vitis vinifera) grape berries. Journal of Integrative Agriculture, 21(4): 1028-1043.

Bernillon S, Biais B, Deborde C, Maucourt M, Cabasson C, Gibon Y, Hansen T H, Husted S, de Vos R C H, Mumm R, Jonker H, Ward J L, Miller S J, Baker J M, Burger J, Tadmor Y, Beale M H, Schjoerring J K, Schaffer A A, Rolin D, et al. 2013. Metabolomic and elemental profiling of melon fruit quality as affected by genotype and environment. Metabolomics, 9, 57–77. 
Botton A, Tonutti P, Ruperti B. 2019. Biology and biochemistry of ethylene. In: Yahia E M, ed., Postharvest Physiology and Biochemistry of Fruit and Vegetables. Woodhead Publishing, Cambridge, UK. pp. 93–112.
Cai F C, Chena K, Xu W, Zhang W, Li X, Ferguson I. 2006. Effect of 1-MCP on postharvest quality of loquat fruit. Postharvest Biology and Technology, 40, 155–162. 
Chen W, Gong L, Guo L, Wang W, Zhang H, Liu X, Yu S, Xiong L, Luo J. 2013. A novel integrated method for large-scale detection, identification, and quantification of widely targeted metabolites: application in the study of rice metabolomics. Molecular Plant, 6, 1769–1780. 
Chohnan S, Murase M, Kurikawa K, Higashi K, Ogata Y. 2014. Antimicrobial activity of pantothenol against staphylococci possessing a prokaryotic type II pantothenate kinase. Microbes and Environments, 29, 224−226. 
Coulon D, Faure L, Salmon M, Wattelet V, Bessoule J J. 2012. Occurrence, biosynthesis and functions of N-acylphosphatidylethanolamines (NAPE): Not just precursors of N-acylethanolamines (NAE). Biochimie, 94, 75–85. 
Diboun I, Mathew S, Al-Rayyashi M, Elrayess M, Torres M, Halama A, Méret M, Mohney R P, Karoly E D, Malek J, Suhre K. 2015. Metabolomics of dates (Phoenix dactylifera) reveals a highly dynamic ripening process accounting for major variation in fruit composition. BMC Plant Biology, 15, 291. 
Ding Y, Chang J, Ma Q, Chen L, Liu S, Jin S, Han J, Xu R, Zhu A, Guo J, Luo Y, Xu J, Xu Q, Zeng Y, Deng X, Cheng Y. 2015. Network analysis of postharvest senescence process in Citrus fruits revealed by transcriptomic and metabolomic profiling. Plant Physiology, 168, 357–376. 
Dixon R A, Strack D. 2003. Phytochemistry meets genome analysis, and beyond. Phytochemistry, 62, 815–816. 
Du Plessis B W. 2008. Cellular factors that affect table grape berry firmness. MSc thesis, Stellenbosch University, South Africa.
Ferrandino A, Guidoni S. 2010. Anthocyanins, flavonols and hydroxycinnamates: An attempt to use them to discriminate Vitis vinifera L. cv ‘Barbera’ clones. European Food Research and Technology, 230, 417–427. 
González-Manzano S, Rivas-Gonzalo J C, Santos-Buelga C. 2004. Extraction of flavan-3-ols from grape seed and skin into wine using simulated maceration. Analytica Chimica Acta, 513, 283–289. 
Hollman P C H. 2009. Absorption, bioavailability, and metabolism of flavonoids. Pharmaceutical Biology, 42, 74–83. 
Iqbal N, Khan N A, Ferrante A, Trivellini A, Francini A, Khan M I R. 2017. Ethylene role in plant growth, development and senescence: Interaction with other phytohormones. Frontiers in Plant Science, 8, 475.
Jin L, Cai Y, Sun C, Huang Y, Yu T. 2019. Exogenous l-glutamate treatment could induce resistance against Penicillium expansum in pear fruit by activating defense-related proteins and amino acids metabolism. Postharvest Biology and Technology, 150, 148–157. 
Karagiannis E, Michailidis M, Karamanoli K, Lazaridou A, Minas I S, Molassiotis A. 2018. Postharvest responses of sweet cherry fruit and stem tissues revealed by metabolomic profiling. Plant Physiology and Biochemistry, 127, 478–484. 
Kim M J, Lee M Y, Shon J C, Kwon Y S, Liu K H, Lee C H, Ku K M. 2019. Untargeted and targeted metabolomics analyses of blackberries - Understanding postharvest red drupelet disorder. Food Chemistry, 300, 125169. 
Lin Y F, Lin H T, Lin Y X, Zhang S, Chen Y H, Jiang X J. 2016. The roles of metabolism of membrane lipids and phenolics in hydrogen peroxide-induced pericarp browning of harvested longan fruit. Postharvest Biology and Technology, 111, 53–61. 
Lin Y F, Lin Y X, Lin H T, Ritenour M A, Shi J, Zhang S, Chen Y H, Wang H. 2017. Hydrogen peroxide-induced pericarp browning of harvested longan fruit in association with energy metabolism. Food Chemistry, 225, 31–36. 
Lohani S, Trivedi P K, Nath P. 2004. Changes in activities of cell wall hydrolases during ethylene-induced ripening in banana: Effect of 1-MCP, ABA and IAA. Postharvest Biology and Technology, 31, 119–126.
Lurie S, Ben-Arie R. 1983. Microsomal membrane changes during the ripening of apple fruit. Plant Physiology, 73, 636–638. 
Maisuthisakul P, Suttajit M, Pongsawatmanit R. 2007. Assessment of phenolic content and free radical-scavenging capacity of some Thai indigenous plants. Food Chemistry, 100, 1409–1418. 
Matsumoto H, Ikoma Y. 2012. Effect of different postharvest temperatures on the accumulation of sugars, organic acids, and amino acids in the juice sacs of Satsuma mandarin (Citrus unshiu Marc.) fruit. Journal of Agricultural and Food Chemistry, 60, 9900−9909. 
Meng J, Xu T, Qin M, Zhuang X, Fang Y, Zhang Z. 2012. Phenolic characterization of young wines made from spine grape (Vitis davidii Foex) grown in Chongyi County (China). Food Research International, 49, 664–671. 
Murota K, Nakamura Y, Uehara M. 2018. Flavonoid metabolism: The interaction of metabolites and gut microbiota. Bioscience, Biotechnology, and Biochemistry, 82, 600–610. 
Neilson A, Ferruzzi M. 2012. Bioavailability and metabolism of bioactive compounds from foods. In: Coulston A, Boushey C, Ferruzzi M, eds., Nutrition in the Prevention and Treatment of Disease. 3rd ed. Elsevier, Oxford, UK. pp. 407–423.
Ni Z J, Hu K D, Song C B, Ma R H, Li Z R, Zheng J L, Fu L H, Wei Z J, Zhang H. 2016. Hydrogen sulfide alleviates postharvest senescence of grape by modulating the antioxidant defenses. Oxidative Medicine and Cellular Longevity, 2016, 1–14.
Obreque-Slier E, Peña-Neira Á, López-Solís R, Zamora-Marín F, Ricardo-da Silva J M, Laureano O. 2010. Comparative study of the phenolic composition of seeds and skins from Carménère and Cabernet Sauvignon grape varieties (Vitis vinifera L.) during ripening. Journal of Agricultural and Food Chemistry, 58, 3591–3599.
OIV (Intemational Organisation of Vine and Wine.) 2019. Statistical Report on World Vitivini culture. International Organisation of Vine and Wine, Paris, France.
Oueslati S, Trabelsi N, Boulaaba M, Legault J, Abdelly C, Ksouri R. 2012. Evaluation of antioxidant activities of the edible and medicinal Suaeda species and related phenolic compounds. Industrial Crops and Products, 36, 513–518. 
Pedreschi R, Franck C, Lammertyn J, Erban A, Kopka J, Hertog M, Verlinden B, Nicola B. 2009. Metabolic profiling of ‘Conference’ pears under low oxygen stress. Postharvest Biology and Technology, 51, 123–130.
Saini M K, Capalash N, Kaur C, Pal Singh S. 2019. Comprehensive metabolic profiling to decipher the influence of rootstocks on fruit juice metabolome of Kinnow (C. nobilis × C. deliciosa). Scientia Horticulturae, 257, 108673. 
Sun X, Zhu A, Liu S, Sheng L, Ma Q, Zhang L, Nishawy E M E, Zeng Y, Xu J, Ma Z. 2013. Integration of metabolomics and subcellular organelle expression microarray to increase understanding the organic acid changes in post-harvest Citrus fruit. Journal of Integrative Plant Biology, 55, 1038–1053. 
Wang H, Chen Y, Sun J, Lin Y, Lin Y, Lin M, Hung Y, Ritenour M, Lin H. 2018. The changes in metabolisms of membrane lipids and phenolics induced by Phomopsis longanae Chi infection in association with pericarp browning and disease occurrence of postharvest longan fruit. Journal of Agricultural and Food Chemistry, 66, 12794–12804. 
Whitaker B D, Smith D L, Green K C. 2001. Cloning, characterization and functional expression of a phospholipase Dα cDNA from tomato fruit. Physiologia Plantarum, 112, 87–94. 
Xu J, Zhang Y, Qi D, Huo H, Dong X, Tian L, Zhang X, Liu C, Cao Y. 2018. Postharvest metabolomic changes in Pyrus ussuriensis Maxim. wild accession ‘Zaoshu Shanli’.  Journal of Separation Science, 41, 4001–4013. 
Yamakawa H, Hakata M. 2010. Atlas of rice grain filling-related metabolism under high temperature: Joint analysis of metabolome and transcriptome demonstrated inhibited starch accumulation and induction of amino acid accumulation. Plant and Cell Physiology, 51, 795−809. 
Yan J, Luo Z, Ban Z, Lu H, Li D, Yang D, Aghdam M S, Li L. 2019. The effect of the layer-by-layer (LBL) edible coating on strawberry quality and metabolites during storage. Postharvest Biology and Technology, 47, 29−38. 
Yi C, Jiang Y M, Shi J, Qu H X, Duan X W, Yang B, Prasad N K, Liu T. 2009. Effect of adenosine triphosphate on changes of fatty acids in harvested litchi fruit infected by Peronophythora litchii. Postharvest Biology and Technology, 54, 159–164. 
Yonekura-Sakakibara K, Higashi Y, Nakabayashi R. 2019. The origin and evolution of plant flavonoid metabolism. Frontiers in Plant Science, 10, 943. 
Yuan Y, Zhao Y, Yang J, Jiang Y, Lu F, Jia Y, Yang B. 2017. Metabolomic analyses of banana during postharvest senescence by 1H-high resolution-NMR. Food Chemistry, 218, 406–412. 
Zenoni S, Amato A, D’Incà E, Guzzo F, Tornielli G B. 2020. Rapid dehydration of grape berries dampens the post-ripening transcriptomic program and the metabolite profile evolution. Horticulture Research, 7, 141.
Zhang B, Shen J Y, Wei W W, Xi W P, Xu C J, Ferguson I, Chen K. 2010. Expression of genes associated with aroma formation derived from the fatty acid pathway during peach fruit ripening. Journal of Agriculture and Food Chemistry, 58, 6157–6165. 
Zhang M, Yuan B, Leng P. 2009. The role of ABA in triggering ethylene biosynthesis and ripening of tomato fruit. Journal of Experimental Botany, 60, 1579–1588.
Zhu Z, Liu R, Li B, Tian S. 2013. Characterisation of genes encoding key enzymes involved in sugar metabolism of apple fruit in controlled atmosphere storage. Food Chemistry, 141, 3323–3328.

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