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Journal of Integrative Agriculture  2022, Vol. 21 Issue (11): 3103-3113    DOI: 10.1016/j.jia.2022.07.059
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Characterization and fine mapping of a semi-rolled leaf mutant srl3 in rice
YU Xiao-qi*, XIE Wei*, LIU He, LIU Wei, ZENG Da-li, QIAN Qian, REN De-yong
State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, P.R.China
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摘要  叶片是植物的主要光合作用器官,最佳的叶片形态有利于塑造理想株型,提高光合效率。在超高产杂交水稻模型中,袁隆平先生将水稻功能叶的形状归纳为直立、狭窄、厚实、卷曲。叶片的适度卷曲有助于其保持直立,减少阳光对叶片的辐射,并降低对叶片的损害,提高植物抵抗力,增加光合产物积累,从而提高作物产量。该研究发现srl3突变体在整个生育期都表现出半卷叶的表型,在分蘖期,其剑叶、第二叶、第三叶的叶片卷曲指数分别平均达到了41%、26%、14%。组织形态学分析发现srl3的剑叶在近轴面上位于中脉、大维管束及小维管束之间的泡状细胞数目和面积均显著性降低。石蜡切片和扫描电镜观察均发现部分小维管束对应的叶片背面缺少厚壁细胞,这些可能都是导致srl3半卷叶表型的原因。另外,我们还检测了一些卷叶相关以及细胞增殖扩展相关基因的表达水平,发现其中大部分基因的表达量都发生了显著性改变,说明SRL3基因很可能与这些卷叶及细胞增殖扩展相关基因有关,共同影响及调控水稻叶片形态。


Moderate leaf rolling can maintain leaf erectness, improve light transmittance in the population, and improve light energy utilization, thereby increasing rice yield.  This study used ethyl methanesulfonate (EMS) to treat Yunjing 17 (YJ17) and obtained a semi-rolled leaf mutant that was named semi-rolled leaf 3 (srl3).  We found that the rolled-leaf phenotype was due to the aberrant development of bulliform cells and the loss of sclerenchymatous cells.  In addition, the shoot and root length of srl3 seedlings differed from the wild type.  The srl3 mutant had significantly lower plant height and seed-setting rate but notably greater tiller number, panicle length, and primary branch number per panicle than the wild type.   Genetic analysis showed that a single recessive nuclear gene defined the srl3 mutant, and it was precisely located in a 144-kb region between two insertion-deletion (InDel) markers, M8 and M19, on chromosome 2.  In this region, no leaf-rolling-related genes have been reported previously.  Thus, the study indicated that SRL3 is a novel leaf-rolling-related gene, and the results laid the foundation for the cloning and functional analysis of the SRL3 gene.

Keywords:  rice        srl3        semi-rolled leaf        bulliform cell        sclerenchymatous cell  
Received: 26 May 2021   Accepted: 06 August 2021
Fund: This work was supported by the National Natural Science Foundation of China (32071993 and 91735304).
About author:  Correspondence QIAN Qian, E-mail:; REN De-yong, E-mail: * These authors contributed equally to this study.

Cite this article: 

YU Xiao-qi, XIE Wei, LIU He, LIU Wei, ZENG Da-li, QIAN Qian, REN De-yong. 2022. Characterization and fine mapping of a semi-rolled leaf mutant srl3 in rice. Journal of Integrative Agriculture, 21(11): 3103-3113.

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