Please wait a minute...
Journal of Integrative Agriculture  2019, Vol. 18 Issue (10): 2264-2271    DOI: 10.1016/S2095-3119(19)62706-1
Horticulture Advanced Online Publication | Current Issue | Archive | Adv Search |
Transcriptome analysis reveals the effects of alkali stress on root system architecture and endogenous hormones in apple rootstocks
LIU Xuan*, LIANG Wei*, LI Yu-xing, LI Ming-jun, MA Bai-quan, LIU Chang-hai, MA Feng-wang, LI Cui-ying
State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest
A&F University, Yangling 712100, P.R.China
Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
Abstract  Soil alkalinity is a major factor that restricts the growth of apple roots. To analyze the response of apple roots to alkali stress, the root structure and endogenous hormones of two apple rootstocks, Malus prunifolia (alkali-tolerant) and Malus hupehensis (alkali-sensitive), were compared. To understand alkali tolerance of M. prunifolia at the molecular level, transcriptome analysis was performed. When plants were cultured in alkaline conditions for 15 d, the root growth of M. hupehensis with weak alkali tolerance decreased significantly. Analysis of endogenous hormone levels showed that the concentrations of indole-3-acetic acid (IAA) and zeatin riboside (ZR) in M. hupehensis under alkali stress were lower than those in the control. However, the trend for IAA and ZR in M. prunifolia was the opposite. The concentration of abscisic acid (ABA) in the roots of the two apple rootstocks under alkali stress increased, but the concentration of ABA in the roots of M. prunifolia was higher than that in M. hupehensis. The expression of IAA-related genes ARF5, GH3.6, SAUR36, and SAUR32 and the Cytokinin (CTK)-related gene IPT5 in M. prunifolia was higher than those in the control, but the expression of these genes in M. hupehensis was lower than those in the control. The expression of ABA-related genes CIPK1 and AHK1 increased in the two apple rootstocks under alkali stress, but the expression of CIPK1 and AHK1 in M. prunifolia was higher than in M. hupehensis. These results demonstrated that under alkali stress, the increase of IAA, ZR, and ABA in roots and the increase of the expression of related genes promoted the growth of roots and improved the alkali tolerance of apple rootstocks.
Keywords:  alkali stress        apple rootstock              endogenous hormone              root architecture        transcriptome analysis  
Received: 28 November 2018   Accepted:
Fund: This study was supported by the earmarked fund for the China Agriculture Research System (CARS-27).
Corresponding Authors:  Correspondence LI Cui-ying, E-mail: lcy1262@nwafu.edu.cn   
About author:  * These authors contributed equally to this study.

Cite this article: 

LIU Xuan, LIANG Wei, LI Yu-xing, LI Ming-jun, MA Bai-quan, LIU Chang-hai, MA Feng-wang, LI Cui-ying. 2019. Transcriptome analysis reveals the effects of alkali stress on root system architecture and endogenous hormones in apple rootstocks. Journal of Integrative Agriculture, 18(10): 2264-2271.

[1] Xiaoxu Shen, Yongtong Tian, Wentao He, Can He, Shunshun Han, Yao Han, Lu Xia, Bo Tan, Menggen Ma, Houyang Kang, Jie Yu, Qing Zhu, Huadong Yin. Gga-miRNA-181-5p family facilitates chicken myogenesis via targeting TGFBR1 to block TGF-β signaling[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2764-2777.
[2] Wajjiha Batool, Justice Norvienyeku, Wei Yi, Zonghua Wang, Shihong Zhang, Lili Lin. Disruption of non-classically secreted protein (MoMtp) compromised conidiation, stress homeostasis, and pathogenesis of Magnaporthe oryzae[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2686-2702.
[3] Gaozhao Wu, Xingyu Chen, Yuguang Zang, Ying Ye, Xiaoqing Qian, Weiyang Zhang, Hao Zhang, Lijun Liu, Zujian Zhang, Zhiqin Wang, Junfei Gu, Jianchang Yang. An optimized strategy of nitrogen-split application based on the leaf positional differences in chlorophyll meter readings[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2605-2617.
[4] Xiaogang He, Zirong Li, Sicheng Guo, Xingfei Zheng, Chunhai Liu, Zijie Liu, Yongxin Li, Zheming Yuan, Lanzhi Li. Epistasis-aware genome-wide association studies provide insights into the efficient breeding of high-yield and high-quality rice[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2541-2556.
[5] Jie Deng, Zi’e Wang, Wenyun Li, Xiaohua Chen, Diqiu Liu. WRKY11 up-regulated dirigent expression to enhance lignin/lignans accumulation in Lilium regale Wilson during response to Fusarium wilt[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2703-2722.
[6] Li Cui, Jianguo Wang, Zhaohui Tang, Zheng Zhang, Sha Yang, Feng Guo, Xinguo Li, Jingjing Meng, Jialei Zhang, Yakov Kuzyakov, Shubo Wan. General and specialized metabolites in peanut roots regulate arbuscular mycorrhizal symbiosis[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2618-2632.
[7] Yao Zhang, Zelong She, Ruolan He, Shuangyan Yao, Xiang Li, Xiaoguang Liu, Xinming Yin, Jizhen Wei, Mengfang Du, Shiheng An. The Ca2+/CaN/ACC and cAMP/PKA/HK signal pathways are required for PBAN-mediated sex pheromone biosynthesis in Conogethes punctiferalis[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2735-2751.
[8] Zihui Liu, Xiangjun Lai, Yijin Chen, Peng Zhao, Xiaoming Wang, Wanquan Ji, Shengbao Xu. Selection and application of four QTLs for grain protein content in modern wheat cultivars[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2557-2570.
[9] Gensheng Zhang, Mudi Sun, Xinyao Ma, Wei Liu, Zhimin Du, Zhensheng Kang, Jie Zhao. Yr5-virulent races of Puccinia striiformis f. sp. tritici possess relative parasitic fitness higher than current main predominant races and potential risk[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2674-2685.
[10] Ming Ju, Guiting Li, Qiuzhen Tian, Hengchun Cao, Qin Ma, Yinghui Duan, Hui Guo, Zhanyou Zhang, Yingying Huang, Huili Wang, Haiyang Zhang, Hongmei Miao. Deletion of a 1,049 bp sequence from the 5´ UTR upstream of the SiHEC3 gene induces a seed non-shattering mutation in sesame  [J]. >Journal of Integrative Agriculture, 2024, 23(8): 2589-2604.
[11] Xiaotong Liu, Siwei Liang, Yijia Tian, Xiao Wang, Wenju Liang, Xiaoke Zhang. Effect of land use on soil nematode community composition and co-occurrence network relationship[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2807-2819.
[12] Jia Chen, Xinran Zhang, Ziqi He, Dongwei Xiong, Miao Long. Damage on intestinal barrier function and microbial detoxification of deoxynivalenol: A review[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2507-2524.
[13] Yuhan Zhao, Chen Qian, Yumei Zhang, Xiande Li, Kamiljon T. Akramov. Food security amid the COVID-19 pandemic in Central Asia: Evidence from rural Tajikistan[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2853-2867.
[14] Myeong-Hyeon Min, Aye Aye Khaing, Sang-Ho Chu, Bhagwat Nawade, Yong-Jin Park. Exploring the genetic basis of pre-harvest sprouting in rice through a genome-wide association study-based haplotype analysis[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2525-2540.
[15] Xin Zhang, Jingjing Wang, Fuli Tan, Haixiu Gao, Shenggen Fan. The potential impact of increased whole grain consumption among Chinese adults on reducing healthcare costs and carbon footprint[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2842-2852.
No Suggested Reading articles found!