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Journal of Integrative Agriculture  2019, Vol. 18 Issue (8): 1851-1858    DOI: 10.1016/S2095-3119(18)62124-0
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Overexpression of G10-EPSPS in soybean provides high glyphosate tolerance
XIAO Pei-ying, LIU Yi, CAO Yue-ping
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P.R.China
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Glyphosate is a highly efficient, broad-spectrum nonspecific herbicide that inhibits the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS)-mediated pathway of shikimic acid.  The screening of glyphosate-resistant EPSPS gene is a major means for the development of new genetically modified glyphosate-resistant transgenic crop.  Currently, the main commercialized glyphosate-resistant soybean contains glyphosate-resistant gene CP4-EPSPS.  In this study, a G10-EPSPS gene was reported providing glyphosate resistance in Zhongdou 32.  Here, G10-EPSPS gene was introduced into soybeans through Agrobacterium-mediated soybean cotyledon node.  PCR, Southern blotting, semi-quantitative RT-PCR, qRT-PCR, and Western blotting were used, and the results revealed that G10-EPSPS had been integrated into the soybean genome and could be expressed steadily at both mRNA and protein levels.  In addition, glyphosate resistance analysis showed that the growth of transgenic soybean had not been affected by concentrations of 900 and 2 700 g a.e. ha–1 of glyphosate.  All the results indicated that G10-EPSPS could provide high glyphosate resistance in soybeans and be applied in production of glyphosate-resistant soybean.
Keywords:  glyphosate        transgenic soybean        G10-EPSPS        glyphosate-resistant  
Received: 09 April 2018   Accepted:
Fund: This research was financially supported by the National Transgenic Major Program, China (2016ZX08004001-04).
Corresponding Authors:  Correspondence CAO Yue-ping, Tel: +86-21-34206940, E-mail:   

Cite this article: 

XIAO Pei-ying, LIU Yi, CAO Yue-ping. 2019.

Overexpression of G10-EPSPS in soybean provides high glyphosate tolerance
. Journal of Integrative Agriculture, 18(8): 1851-1858.

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