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Journal of Integrative Agriculture  2017, Vol. 16 Issue (03): 551-558    DOI: 10.1016/S2095-3119(16)61458-2
Crop Genetics · Breeding · Germplasm Resources Advanced Online Publication | Current Issue | Archive | Adv Search |
Development of glyphosate-tolerant transgenic cotton plants harboring the G2-aroA gene
ZHANG Xiao-bing1, 2*, TANG Qiao-ling1*, WANG Xu-jing1, WANG Zhi-xing1

1 Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

2 Biology Institute, Hebei Academy of Sciences, Shijiazhuang 050051, P.R.China

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Abstract  Given that glyphosate weed control is an effective strategy to reduce costs and improve economic outcomes of agricultural production in China, the development of glyphosate-resistant cotton holds great promise.  Using an Agrobacterium-mediated transformation method, a new G2-aroA gene that encodes 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) was transformed into cotton cultivar K312.  The transgenic cotton plants were regenerated from a callus tissue culture via kanamycin selection.  Ten regenerated cotton plants were obtained and allowed to flower normally to produce fruit.  The results from polymerase chain reaction (PCR) and Southern and Western blot analyses indicated that the target gene was integrated into the cotton chromosome and was expressed effectively at the protein level.  The glyphosate tolerance analysis showed that the transgenic cotton had a high resistance to glyphosate.  Further, even cotton treated with 45.0 mmol L–1 of glyphosate was able to slowly grow, bloom and seed.  The transgenic cotton may be used for cotton breeding research of glyphosate-tolerant cotton.
Keywords:   cotton (Gossypium hirsutum L.)      Agrobacterium-mediated method      glyphosate, G2-aroA      genetic transformation  
Received: 15 March 2016   Accepted:

This work was supported by the Genetically Modified Major Projects, China (2012ZX08011-003 and 2014ZX08011-004B).

Corresponding Authors:  WANG Xu-jing, Tel: +86-10-82106124, E-mail:; WANG Zhi-xing, Tel: +86-10-82106102, E-mail:   
About author:  ZHANG Xiao-bing, E-mail:

Cite this article: 

ZHANG Xiao-bing, TANG Qiao-ling, WANG Xu-jing, WANG Zhi-xing. 2017. Development of glyphosate-tolerant transgenic cotton plants harboring the G2-aroA gene. Journal of Integrative Agriculture, 16(03): 551-558.

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