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Journal of Integrative Agriculture  2018, Vol. 17 Issue (07): 1612-1619    DOI: 10.1016/S2095-3119(17)61765-9
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An EMS mutant library for cucumber
CHEN Chen1, 2, 4, CUI Qing-zhi2, HUANG San-wen3, WANG Shen-hao3, LIU Xiao-hong2, LU Xiang-yang1, 4, 5, CHEN Hui-ming1, 2, 4, TIAN Yun1, 4, 5
1 College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, P.R.China
2 Hunan Vegetable Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, P.R.China
3 Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture/Sino-Dutch Joint Laboratory of Horticultural Genomics, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
4 Key Laboratory for Agricultural Biochemistry and Biotransformation of Hunan Provincial University, Changsha 410128, P.R.China
5 Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Changsha 410128, P.R.China
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Abstract  
Cucumber is an important vegetable crop and a model crop for the study of sex expression in plants.  However, the genomic resources and tools for functional genomics studies in cucumber are still limited.  In this paper, we conducted ethyl methyl sulfone (EMS) mutagenesis in the northern China ecotype cucumber inbred line 406 to construct a mutant library.  We optimized the conditions of EMS mutagenesis on inbred line 406 which included treatment of seeds at 1.5% EMS for 12 h.  We obtained a number of mutant lines showing inheritable morphological changes in plant architecture, leaves, floral organs, fruits and other traits through M1, M2 and M3 generations.  The F2 segregating populations were constructed and analyzed.We found that a short fruit mutant and a yellow-green fruit peel mutant were both under the control of a single recessive gene, respectively.  These results provide valuable germplasm resources for the improvement of cucumber genetics and functional genomic research.
Keywords:  cucumber ')" href="#">
cucumber        EMS      mutant library        traits
  
Received: 27 July 2017   Accepted:
Fund: This study was sponsored by the National Key R&D Program of China (2016YFD0100307), the National Natural Science Foundation of China (31471871) and the Construct Program of the Key Disciplines in Hunan Province, China.
Corresponding Authors:  Correspondence CHEN Hui-ming, E-mail: huiminghm@163.com; TIAN Yun, E-mail: tianyun@hunau.edu.cn    
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CHEN Chen
CUI Qing-zhi
HUANG San-wen
WANG Shen-hao
LIU Xiao-hong
LU Xiang-yang
CHEN Hui-ming
TIAN Yun

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CHEN Chen, CUI Qing-zhi, HUANG San-wen, WANG Shen-hao, LIU Xiao-hong, LU Xiang-yang, CHEN Hui-ming, TIAN Yun. 2018. An EMS mutant library for cucumber. Journal of Integrative Agriculture, 17(07): 1612-1619.

Boualem A, Fleurier S, Troadec C, Audigier P, Kumar A P, Chatterjee M, Alsadon A A, Sadder M T, Wahb-Allah M A, Al-Doss A A, Bendahmane A. 2014. Development of a Cucumis sativus TILLinG platform for forward and reverse genetics. PLoS ONE, 9, e97963.
Boualem A, Troadec C, Camps C, Lemhemdi A, Morin H, Sari M A, Fraenkel-Zagouri R, Kovalski I, Dogimont C, Perl-Treves R, Bendahmane A. 2015. A cucurbit androecy gene reveals how unisexual flowers develop and dioecy emerges. Science, 350, 688–691.
Chen H, Sun J, Li S, Cui Q, Zhang H, Xin F, Wang H, Lin T, Gao D, Wang S, Li X, Wang D, Zhang Z, Xu Z, Huang S. 2016. An ACC oxidase gene essential for cucumber carpel development. Molecular Plant, 9, 1315–1327.
Chen H, Tian Y, Lu X, Liu X. 2011. The inheritance of two novel subgynoecious genes in cucumber (Cucumis sativus L.). Scientia Horticulturae, 127, 464–467.
Chen Y L, Liang H L, Ma X L, Lou S L, Xie Y Y, Liu Z L, Chen L T, Liu Y G. 2013. An efficient rice mutagenesis system based on suspension-cultured cells. Journal of Integrative Plant Biology, 55, 122–130.
Fraenkel R, Kovalski I, Troadec C, Bendahmane A, Perl-Treves R. 2014. Development and evaluation of a cucumber TILLING population. BMC Research Notes, 7, 846.
Galpaz N, Burger Y, Lavee T, Tzuri G, Sherman A, Melamed T, Eshed R, Meir A, Portnoy V, Bar E, Shimoni-Shor E, Feder A, Saar Y, Saar U, Baumkoler F, Lewinsohn E, Schaffer A A, Katzir N, Tadmor Y. 2013. Genetic and chemical characterization of an EMS induced mutation in Cucumis melo CRTISO gene. Archives of Biochemistry and Biophysics, 539, 117–125.
Greene E A, Codomo C A, Taylor N E, Henikoff J G, Till B J, Reynolds S H, Enns L C, Burtner C, Johnson J E, Odden A R, Comai L, Henikoff S. 2003. Spectrum of chemically induced mutations from a large-scale reverse-genetic screen in Arabidopsis. Genetics, 164, 731–740.
Henry I M, Nagalakshmi U, Lieberman M C, Ngo K J, Krasileva K V, Vasquez-Gross H, Akhunova A, Akhunov E, Dubcovsky J, Tai T H, Comai L. 2014. Efficient genome-wide detection and cataloging of EMS-induced mutations using exome capture and next-generation sequencing. The Plant Cell, 26, 1382–1397.
Huang S, Li R, Zhang Z, Li L, Gu X, Fan W, Lucas W J, Wang X, Xie B, Ni P, Ren Y, Zhu H, Li J, Lin K, Jin W, Fei Z, Li G, Staub J, Kilian A, van der Vossen E A, et al. 2009. The genome of the cucumber, Cucumis sativus L. Nature Genetics, 41, 1275–1281.
Li Z, Jiang L, Ma Y, Wei Z, Hong H, Liu Z, Lei J, Liu Y, Guan R, Guo Y, Jin L, Zhang L, Li Y, Ren Y, He W, Liu M, Htwe N M, Liu L, Guo B, Song J, et al. 2017. Development and utilization of a new chemically-induced soybean library with a high mutation density. Journal of Integrative Plant Biology, 59, 60–74.
Lin T, Wang S, Zhong Y, Gao D, Cui Q, Chen H, Zhang Z, Shen H, Weng Y, Huang S. 2016. A truncated F-box protein confers the dwarfism in cucumber. Journal of Genetics and Genomics, 43, 223–226.
Liu H, Jiao J, Liang X, Liu J, Meng H, Chen S, Li Y, Cheng Z. 2016. Map-based cloning, identification and characterization of the w gene controlling white immature fruit color in cucumber (Cucumis sativus L.). Theoretical and Applied Genetics, 129, 1247–1256.
Lo S F, Fan M J, Hsing Y I, Chen L J, Chen S, Wen I C, Liu Y L, Chen K T, Jiang M J, Lin M K, Rao M Y, Yu L C, Ho T H, Yu S M. 2016. Genetic resources offer efficient tools for rice functional genomics research. Plant Cell and Environment, 39, 998–1013.
Menda N, Semel Y, Peled D, Eshed Y, Zamir D. 2004. In silico screening of a saturated mutation library of tomato. The Plant Journal, 38, 861–872.
Sagan M, Morandi D, Tarenghi E, Duc G. 1995. Selection of nodulation and mycorrhizal mutants in the model plant Medicago truncatula (Gaertn.) after γ-ray mutagenesis. Plant Science, 111, 63–71.
Shang Y, Ma Y, Zhou Y, Zhang H, Duan L, Chen H, Zeng J, Zhou Q, Wang S, Gu W, Liu M, Ren J, Gu X, Zhang S, Wang Y, Yasukawa K, Bouwmeester H J, Qi X, Zhang Z, Lucas W J, et al. 2014. Biosynthesis, regulation, and domestication of bitterness in cucumber. Science, 346, 1084–1088.
Staub J E, Fredrick L, Marty T L. 1987. Electrophoretic variation in cross-compatible wild diploid species of Cucumis. Canadian Journal of Botany, 65, 792–798.
Tsuda M, Kaga A, Anai T, Shimizu T, Sayama T, Takagi K, Machita K, Watanabe S, Nishimura M, Yamada N, Mori S, Sasaki H, Kanamori H, Katayose Y, Ishimoto M. 2015. Construction of a high-density mutant library in soybean and development of a mutant retrieval method using amplicon sequencing. BMC Genomics, 16, 1014.
Wang L, Cao C, Zheng S, Zhang H, Liu P, Ge Q, Li J, Ren Z. 2017. Transcriptomic analysis of short-fruit 1 (sf1) reveals new insights into the variation of fruit-related traits in Cucumis sativus. Scientific Reports, 7, 2950.
Wang S, Ku S S, Ye X, He C, Kwon S Y, Choi P S. 2015. Current status of genetic transformation technology developed in cucumber (Cucumis sativus L.). Journal of Integrative Agriculture, 14, 469–482.
Wang X, Gao D, Sun J, Liu M, Lun Y, Zheng J, Wang S, Cui Q, Wang X, Huang S. 2016. An exon skipping in a SEPALLATA-Like gene is associated with perturbed floral and fruits development in cucumber. Journal of Integrative Plant Biology, 58, 766–771.
Zhou Q, Wang S, Hu B, Chen H, Zhang Z, Huang S. 2015. An accumulation and replication of chloroplasts 5 gene mutation confers light green peel in cucumber. Journal of Integrative Plant Biology, 57, 936–942.
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