|
|
|
|
|
| Differential effects of insecticides on mitochondrial membrane fluidity and ATPase activity between the wolf spider and the rice stem borer |
| LI Hai-ping, CHANG Jing, FENG Tao, GAO Xi-wu |
1、Department of Entomology, China Agricultural University, Beijing 100193, P.R.China
2、College of Agronomy, Inner Mongolia Agricultural University, Hohhot 010019, P.R.China
3、College of Life Science, Jianghan University, Wuhan 430056, P.R.China |
|
|
|
摘要 Differential effects of methamidophos and three pyrethroids on ATPase activity and membrane fluidity of mitochondria were investigated between the wolf spider (Pirata subpiraticus (Boes. et Str.)) and the rice stem borer (Chilo suppressalis (Walker)). Based on a comparison of LD50 values, the toxicities of the tested insecticides were higher to the wolf spider than to the rice stem borer. Cyhalothrin at 1×10–4 mmol L–1 caused inhibition of the mitochondrial Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities, and it’s inhibitions on Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities were significantly higher in the wolf spider (44 and 28%) than in the rice stem borer (19 and 11%). Methamidophos at 1×10–4 mmol L–1 decreased Ca2+-Mg2+-ATPase activity by 16 and 27% in the wolf spider and the rice stem borer, respectively, but no significant effect on the specific activity of Na+-K+-ATPase was observed. The DPH (1,6-diphenyl-1,3,5-hexatriene) fluorescence polarization values of mitochondrial membranes were not significantly affected by methamidophos in either species. However, cyhalothrin and alpha-cypermethrin induced the values of DPH polarization of mitochondrial membrane increasing with the concentration of cyhalothrin and alpha-cypermethrin from 20 to 100 μmol L–1 in the rice stem borer and the wolf spider. Effect of ethofenprox on fluidity of the wolf spider and the rice stem borer was contrary. These results suggest that both inhibition of membrane ATPase and changes of membrane fluidity could be appended to the action mechanisms of pyrethroid insecticides.
Abstract Differential effects of methamidophos and three pyrethroids on ATPase activity and membrane fluidity of mitochondria were investigated between the wolf spider (Pirata subpiraticus (Boes. et Str.)) and the rice stem borer (Chilo suppressalis (Walker)). Based on a comparison of LD50 values, the toxicities of the tested insecticides were higher to the wolf spider than to the rice stem borer. Cyhalothrin at 1×10–4 mmol L–1 caused inhibition of the mitochondrial Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities, and it’s inhibitions on Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities were significantly higher in the wolf spider (44 and 28%) than in the rice stem borer (19 and 11%). Methamidophos at 1×10–4 mmol L–1 decreased Ca2+-Mg2+-ATPase activity by 16 and 27% in the wolf spider and the rice stem borer, respectively, but no significant effect on the specific activity of Na+-K+-ATPase was observed. The DPH (1,6-diphenyl-1,3,5-hexatriene) fluorescence polarization values of mitochondrial membranes were not significantly affected by methamidophos in either species. However, cyhalothrin and alpha-cypermethrin induced the values of DPH polarization of mitochondrial membrane increasing with the concentration of cyhalothrin and alpha-cypermethrin from 20 to 100 μmol L–1 in the rice stem borer and the wolf spider. Effect of ethofenprox on fluidity of the wolf spider and the rice stem borer was contrary. These results suggest that both inhibition of membrane ATPase and changes of membrane fluidity could be appended to the action mechanisms of pyrethroid insecticides.
|
|
Received: 12 December 2014
Accepted:
|
| Fund: This work was supported by the National Basic Research Program of China (2012CB114103). |
|
Corresponding Authors:
GAO Xi-wu, Tel/Fax: +86-10-62732974, E-mail: gaoxiwu@263.net.cn
E-mail: gaoxiwu@263.net.cn
|
| About author: LI Hai-ping, Tel: +86-471-4308472, E-mail: lihaiping5820@hotmail.com; |
Cite this article:
LI Hai-ping, CHANG Jing, FENG Tao, GAO Xi-wu.
2015.
Differential effects of insecticides on mitochondrial membrane fluidity and ATPase activity between the wolf spider and the rice stem borer. Journal of Integrative Agriculture, 14(12): 2574-2580.
|
| Al-Rajhi D H. 1990. Properties of Ca2+-Mg2+-ATPase fromrat brain and its inhibition by pyrethroids. PesticideBiochemistry and Physiology, 37, 116-120Antunes-Maderia M C, Maderia V M C. 1979. Interaction ofinsecticides with lipid membranes. Biochimicaet BiophysicaActa, 550, 384-392Antunes-Maderia M C, Maderia V M C. 1984. Partition ofparathion in synthetic and native membranes. BiochimicaetBiophysica Acta, 778, 49-56Antunes-Maderia M C, Maderia V M C. 1989. Membranefluidity as affected by the insecticide lindane. BiochimicaetBiophysica Acta, 982, 161-166Bradford M M. 1976. A rapid and sensitive method for thequantitation of microgram quantities of protein utilizing theprinciple of protein-dye binding. Analytical Biochemistry,72, 248-254Braguini W L, Cadena S M S C, Carnieri E G S, Rocha M E M,Oliveira M B M. 2004. Effects of deltamethrin on functionof rat mitochondria and on native and synthetic modelmembrane. Toxicology Letters, 152, 191-202Brown M D, Dudeja P K, Brasitus T A. 1988. S-adenosyl-Lmethioninemodulates Na+/K+-ATPase activity in rat colonicbasolateral membranes. Journal of Biochemistry, 251,215-222Cheng E Y, Cutkomp L K. 1977. The sensitivity of mitochondrialMg2+-ATPase to DDT and analogs at different temperatures.Analytical Biochemistry, 77, 360-366Cheng X, Chang C, Dai S M. 2010. Responses of striped stemborer, Chilo suppressalis (Lepidoptera: Pyralidae), fromTaiwan to a range of insecticides. Pesticides ManagementScience, 66, 762-766Cinzia N, Franco C, Giancarlo F, Gabbianelli R. 2003. Differenteffects of Type I and Type II pyrethroids on erythrocyteplasma membrane properties and enzymatic activity in rats.Toxicology, 191, 233-244Clark J M, Matsumra F. 1982. Two different types of inhibitoryeffects of pyrethroid on nerve Ca2+- and Ca2+-Mg2+-ATPaseactivity in the squid Loligopealei. Pesticide Biochemistry andPhysiology, 18, 180-190Croft B A, Brown A W A. 1982. Responses of arthropod naturalenemies to insecticides. Annual Review of Entomology,20, 285-335Easterbrook M A. 1997. A field assessment of the effects ofinsecticides on the beneficial fauna of strawberry. CropProtection, 16, 147-152Edwards R, Millburn P. 1985. The Metabolism and Toxicity ofInsecticides in Fish. Insecticides, vol.5. John Wiley andSons, New York, USA. pp. 249-269Fan Y R. 1993. The possibility of pyrethroids using in paddyrice in China from the status of pyrethroids in the paddy ricein Philippine. Pesticides of World, 15, 26-31 (in Chinese)FAO. 1980. Method for larvae of the rice stem borer (Chilosuppressalis Walker). FAO Method No.3. In: PestResistance to Pesticides and Crop Loss Assessment. FAO,Rome. pp. 25-28Feng T, Peng Y, Liu F, Fang M, Chen J. 2007. Susceptibilityto pesticides of different geographic populations ofHylyphantes graminicola and relative fitness of susceptibleand resistant populations. Acta Ecologica Sinica, 27,5093-5097 (in Chinese)Hu J, Chen W, Zhang Z Z, Zheng X S, Jin J C, Su J, Gao CF, Shen J L. 2010. Insecticide resistance monitoring ofChilosuppressalis in the drainage area of the Yangtzeriver, China. China Journal of Rice Science, 24, 509-515(in Chinese)Husain R, Malaviya M, Seth P K. 1994. Effect of deltamethrinon regional brain polyamines and behavior in young rats.Pharmacology & Toxicology, 74, 211-215Kakko I, Toimela T, Tähti H. 2000. Piperonylbutoxide potentiatesthe synaptosome ATPase inhibiting effect of pyrethrin.Chemosphere, 40, 301-305Kakko I, Toimela T, Tähti H. 2003. The synaptosomalmembrane bound ATPase as a target for the neurotoxiceffects of pyrethroids, permethrin and cypermethrin.Chemosphere, 51, 475-480Keeffe, E B, Scharschmidt B T, Blankenship N M, Ockner R K J.1979. Studies of relationships among bile flow, liver plasmamembrane Na+/K+-ATPase, and membrane microviscosity in the rat. Medecine Clinique et Experimentale, 64,1590-1598Kim C, Leo M A, Lowe N, Lieber C S. 1988. Effects of vitamin Aand ethanol on liver plasma membrane fluidity. Hepatology,8, 735-741Li H P, Feng T, Liang P, Shi X Y, Gao X W, Jiang H. 2006a.Effect of temperature on toxicity of pyrethroids and the invitro sensitivity of Na+-K+-ATPase and Ca2+-Mg2+-ATPasein Chilo suppressalis (Lepidoptera: Pyralidae). PesticideBiochemistry and Physiology, 86, 151-156Li H P, Feng T, Shi X Y, Liang P, Zhang Q, Gao X W.2009. Effects of pyrethroids and endosulfanon fluidity ofmitochondria membrane in Chilo suppressalis (Walker),Pesticide Biochemistry and Physiology, 95, 72-76Li H P, Feng T, Tao L M, Liu X, Jiang H, Lin R H, Gao X W.2006b. Inhibition of nine common insecticides on ATPaseactivity from mitochondria of Chilo supperssalis Walker.Acta Entomologica Sinica, 49, 113-118 (in Chinese)Li H P, Shi X Y, Liang P, Gao X W. 2012. Effects of insecticideson fluidity of mitochondrial membrane by fluorescence probeDPH in Chilo suppressalis. Chinese Journal of AppliedEntomology, 49, 342-347 (in Chinese)Luo M, Bodnaryk R P. 1988. The effect of insecticides onCa2+-Mg2+-ATPase and the ATP-dependent calcium pumpin moth brain synaptosomes and synaptosome membranevesicle from the bertha armyworm, MamestraconfigurataWLK. Pesticide Biochemistry and Physiology, 30, 155-165Mansour F. 1987. Effect of pesticides on spiders occurring onapple and citrus in Israel. Phytoparasitica, 15, 43-50Mullin C A, Croft B A, Strickler K, Matsumura F, Miller J R. 1982.Detoxification enzyme difference between a herbivorousand predatory mite. Science, 217, 1270-1272Muszbek L, Szabq T, Fesue L. 1977. A highly sensitivemethod for the measurement of ATPase activity. AnalyticalBiochemistry, 77, 286-288Narahashi T. 1992. Nerve membrane Na+ channels as targetsof insecticides. Trends in Pharmacological Science, 13,236-241Narahashi T, Ginsburg K S, Nagata K, Song J H, TatebayashiH. 2000. Ion channels as targets for insecticides.Neurotoxicology, 19, 581-590Nasuti C, Cantalamessa F, Falcioni G, Gabbianelli R. 2003.Different effects of Type I and Type II pyrethroids onerythrocyte plasma membrane properties and enzymaticactivity in rats. Toxicology, 191, 233-244Ogata N, Ohishi Y. 2002. Molecular diversity of structure andfunction of the voltage gated Na+ channels. The JapaneseJournal of Pharmacology, 88, 365-377Peng Y, Han Z J, Wang Y C. 2000. Toxicity of four insecticidesto Hylyphantes graminicola and Misumenops tricuspidatus(Arachnia: Araneae). Journal of Nanjing AgriculturalUniversity, 23, 117-119 (in Chinese)Ramon R, Andres M M, Rodrigo V G, Juan P B, Leonardo V.2014. Modulation of (Na, K)-ATPase activity by membranefatty acid composition: Therapeutic implications in humanhypertension. Clinical and Experimental Hypertension, 36,17-26Rosita G, Giancarlo F, Cinzia N. 2002. Cypermethrin-inducedplasma membrane perturbation on erythrocytes fromrats: Reduction of fluidity in the hydrophobic core and inglutathione peroxidase activity. Toxicology, 175, 91-101Sarkar S N, Balasubramanian S V, Sikdar S K. 1993. Effect offenvalerate, a pyrethroid insecticide on membrane fluidity.Biochimicaet Biophysica Acta, 1147, 137-142Schachter D. 1984. Fluidity and function of hepatocyte plasmamembranes. Hepatology, 4, 140-151Soderlund D M, Bloomquist J R. 1989. Neurotoxic actions ofpyrethroid insecticides. Annual Review of Entomology, 34,77-96Soderlund D M, Clark J M, Sheets P L, Mullin L S, Piccirillo VJ, Sargent D, Stevens J T, Weiner M L. 2002. Mechanismsof pyrethroid neurotoxicity: Implications for cumalative riskassessment. Toxicology, 171, 3-9Song J H, Narahashi T. 1996. Differential effects of thepyrethroidetramethrin on tetrodotoxin-sensitive andtetrodotoxin-resistant single sodium channels. BrainResearch, 712, 258-264Su J Y, Zhang Z Z, Wu M, Gao C F. 2014. Geographicsusceptibility of Chilo suppressalis Walker (Lepidoptera:Crambidae) to chiorantraniliprole in China. Pest ManagementScience, 70, 989-995Toshio N. 1979. Neurotoxicology of Insecticides andPheromones. Plenum Press, New York, USA. pp. 158-170Vani T, Saharan N, Makherjee S C, Ranjan R, Kumar R,Brahmchari R K. 2011. Deltamethrin induced alternationof hematological and biochemical parameters in fingerlingsCatlacatla (Ham) and their amelioration by dietarysupplement of vitamin C. Pesticide Biochemistry andPhysiology, 101, 16-20Vershoyle R D, Aldridge W N. 1980. Structure-activityrelationships of some pyrethroids in rats. Archives ofToxicology, 45, 325-329Wick M, Freier B. 2000. Long-term effects of an insecticideapplication on non-target arthropods in winter wheat - Afield study over 2 seasons. Journal of Pest Science, 73,61–69.Zibaee A, Sendi J, Alinia F, Etebari K. 2009. Diazinon resistancein different selected strains of Chilo suppressalis Walker(Lepidoptera: Pyralidae), rice striped stem borer, in the northof Iran. Journal of Economic Entomology, 102, 1189–1196. |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
