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Journal of Integrative Agriculture  2022, Vol. 21 Issue (4): 1058-1070    DOI: 10.1016/S2095-3119(21)63652-3
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Kiwifruit (Actinidia chinensis ‘Hongyang’) cytosolic ascorbate peroxidases (AcAPX1 and AcAPX2) enhance salinity tolerance in Arabidopsis thaliana
GUO Xiu-hong1*, HE Yan2*, ZHANG Yu3, WANG Yi2, HUANG Sheng-xiong3, LIU Yong-sheng1, 2, 3, LI Wei2
1 Key Laboratory for Bio-resource and Eco-environment of Ministry of Education, College of Life Sciences/State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu 610064, P.R.China
2 School of Horticulture, Anhui Agricultural University, Hefei 230036, P.R.China
3 School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, P.R.China
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Abstract  Ascorbate peroxidase (APX) plays a key role in scavenging reactive oxygen species (ROS) in higher plants.  However, there is very little information available on the APXs in kiwifruit (Actinidia), which is an economically and nutritionally important horticultural crop with exceptionally high ascorbic acid (AsA) accumulation.  This study aims to identify and characterize two cytosolic APX genes (AcAPX1 and AcAPX2) derived from A. chinensis ‘Hongyang’.  The constitutive expression pattern was determined for both AcAPX1 and AcAPX2, and showed relatively higher expression abundances of AcAPX1 in leaf and AcAPX2 in root.  Transcript levels of AcAPX1 and AcAPX2 were increased in kiwifruit roots treated with NaCl.  Subcellular localization assays using GFP-fusion proteins in Arabidopsis protoplasts showed that both AcAPX1 and AcAPX2 are targeted to the cytosol.  Recombinant AcAPX1 or AcAPX2 proteins were successfully expressed in the prokaryotic expression system and their individual ascorbate peroxidase activities were determined.  Finally, constitutive over-expression of AcAPX1 or AcAPX2 could dramatically increase total AsA, glutathione level and salinity tolerance under NaCl stress in Arabidopsis thaliana.  Our findings revealed that cytosolic AcAPX1/2 may play an important protective role in the responses to unfavorable environmental stimuli in kiwifruit.
Keywords:  Actinidia       ascorbic acid        ascorbate peroxidase        salinity  
Received: 04 October 2020   Accepted: 18 February 2021
Fund: This research was funded by the National Natural Science Foundation of China (31972474), the Natural Science Research Program of Universities of Anhui Province, China (K1832004), the Leading Talent Group Funding of Anhui Province, China (WRMR-2020-75), the Natural Science Foundation of Anhui Province, China (19232002) and the Anhui Agriculture University Shennong Scholar Project, China (RC321901).

About author:  GUO Xiu-hong, E-mail:; HE Yan, E-mail:; Correspondence LI Wei, E-mail:; LIU Yong-sheng, E-mail: * These authors contributed equally to this study.

Cite this article: 

GUO Xiu-hong, HE Yan, ZHANG Yu, WANG Yi, HUANG Sheng-xiong, LIU Yong-sheng, LI Wei. 2022. Kiwifruit (Actinidia chinensis ‘Hongyang’) cytosolic ascorbate peroxidases (AcAPX1 and AcAPX2) enhance salinity tolerance in Arabidopsis thaliana. Journal of Integrative Agriculture, 21(4): 1058-1070.

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