Please wait a minute...
Journal of Integrative Agriculture  2020, Vol. 19 Issue (10): 2451-2457    DOI: 10.1016/S2095-3119(20)63282-8
Special Issue: 园艺-栽培生理/资源品质合辑Horticulture — Physiology · Biochemistry · Cultivation
Horticulture Advanced Online Publication | Current Issue | Archive | Adv Search |
A new species of Malus in China, Malus shizongensis Liu sp. nov
LIU Zhen-zhong1, LI Zhong-xing2, GAO Hua1, BAO Cha-na2 
1 College of Horticulture, Northwest A&F University, Yangling 712100, P.R.China
2 State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R.China
Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
Based on morphological, molecular biological, and molecular systematic studies, we describe here a new species of Malus from Yunnan, China.  We compared the morphology of this new species, Malus shizongensis Liu sp. nov, with three Malus species, including M. hupehensis, M. baccata, and M. micromalus.  Although the appearance of M. shizongensis was similar to these three species, it differed in height, branch color, branch hair, and flower color.  To better identify the taxonomy of this new species, genome of M. shizongensis and that of seven Malus species, including M. prunifolia, M. sylvestris, M. sieversii, M. hupehensis, M. baccata, M. robusta, and M. micromalus were analyzed.  A phylogenetic tree based on genome analysis indicated that M. shizongensis was close to M. hupehensis.  Furthermore, M. shizongensis had its species-specific SNPs, and the number of species-specific SNPs was similar to that of three close species (M. hupehensis, M. baccata, and M. micromalus).  Based on the above information, we named this new species as M. shizongensis Liu sp. nov.
Keywords:  Malus        new species        taxonomy  
Received: 30 December 2019   Accepted:
Fund: This work was supported by the Shaanxi Provincial Science and Technology Project, China (K3310216042, K3360217106).
Corresponding Authors:  Correspondence BAO Cha-na, Tel/Fax: +86-29-87082648, E-mail:   
About author:  LIU Zhen-zhong, E-mail:;

Cite this article: 

LIU Zhen-zhong, LI Zhong-xing, GAO Hua, BAO Cha-na. 2020. A new species of Malus in China, Malus shizongensis Liu sp. nov. Journal of Integrative Agriculture, 19(10): 2451-2457.

Daccord N, Celton J, Linsmith G, Becker C, Choisne N, Schijlen E, Geest H, Bianco L, Micheletti D, Velasco R, Pierro E, Gouzy J, Rees D, Guérif P, Muranty H, Durel C, Laurens F, Lespinasse Y, Gaillard S, Aubourg S, Quesneville H, Weigel D, Weg E, Troggio M, Bucher E. 2017. High-quality de novo assembly of the apple genome and methylome dynamics of early fruit development. Nature Genetics, 49, 1099–1106.
Dirlewanger E, Cosson P, Boudehri K, Renaud C, Capdeville G, Tauzin Y, Laigret F, Moing A. 2006. Development of a second-generation geneticlinkage map for peach [Prunus persica (L.) Batsch] and characterization of morphological traits affecting flower and fruit. Tree Genetics & Genomes, 3, 1–13.
Duan N B, Bai Y, Sun H H, Wang N, Ma Y M, Li M J, Wang X, Jiao C, Legall N, Mao L Y, Wan S B, Wang K, He T, Feng S Q, Zhang Z Y, Mao Z Q, Shen X, Chen X L, Jiang Y M, Wu S J, et al. 2017. Genome re-sequencing reveals the history of apple and supports a two-stage model for fruit enlargement. Nature Communications, 8, 249.
Li H, Durbin R. 2010. Fast and accurate long-read alignment with Burrows–Wheeler transform. Bioinformatics, 26, 589–595.
Han Y P, Chagne D, Gasic K, Rikkerink E H A, Beever J E, Gardiner S E, Korban S S. 2009. BAC-end sequence-based SNPs and Bin mapping for rapid integration of physical and genetic maps in apple. Genomics, 93, 282–288.
Hua L Y, Wang X, Qian G Z. 2019. Karyotype analysis of Malus Mill. Journal of Liaocheng University (Natural Science Edition), 32, 97–110.
Koehne E. 1893. Deutsche Dendrologie. Verlag von Ferdinand Enke, Stuttgart.  (in German)
Kumar S, Stecher G, Tamura K. 2016. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology & Evolution, 33, 1870.
Li Y N. 1996a. Review on the research progress of species and classification of apples in the world. Journal of Fruit Science, (S1), 63–81.  (in Chinese)
Li Y N. 1996b. Discussion on the new classification system of apple genus in modern world. Journal of Fruit Science, (S1), 82–92.  (in Chinese)
Li Y N. 2001. Germplasm Resources of Malus. China Agricultural Press, China. (in Chinese)
Li Y N, Li X L. 1989. The genetic relationship among the apple subgroup Pumilae Rehd in Eumalus Zabel of the apple genus. The 60th Anniversary and Annual Meeting of the Chinese Horticultural Society, Chinese Society of Horticulture.  (in Chinese)
Liu Y, Yu X Q, Zhang A N, Wang F M, Liu G L. 2016. Application of high-throughput genome sequencing in crop gene discovery and mapping. Acta Agriculturae Shanghai, 32, 171–175.
Lu Y Q, Jiao M L, Cao Y C. 2015. Climate characteristic analysis of loess plateau areas in Qingyang, Gansu in recent 35 years. Chinese Agricultural Science Bulletin, 31, 181–190.
Lu Z H, Niu L, Chagne D, Cui G C, Pan L, Foster T, Zhang R P, Zeng W F, Wang Z Q. 2016. Fine mapping of the temperature - sensitive semi - dwarf (Tssd) locus regulating the internode length in peach (Prunus persica). Molecular Breeding, 36, 1–11.
Ma J H, Li J Y, Ma E D, Pang T, Lu X P. 2013. Research on the climate ecology zoning of Yunnan gold corridor tobacco cultivation region. Chinese Agricultural Science Bulletin, 29, 70–75.
Mckenna A, Hanna M, Banks E, Sivachenko A, Cibulskis K. Kernytsky  A,  Garimella  K, Altshuler D, Gabriel S, Daly M, DePristo M. 2010. The genome analysis toolkit: A MapReduce framework for analyzing next-generation DNA sequencing data. Genome Research, 20, 1297–1303.
Muranty H, Jorge V, Bastien C, Lepoittevin C, Bouffier L, Sanchez L. 2014. Potential for marker-assisted selection for forest tree breeding: Lessons from 20 years of MAS in crops. Tree Genetics & Genomes, 10, 1491–1510.
Rehder A. 1920. New species, varieties and combinations from the herbarium and the collections of the arnold arboretum. Journal of the Arnold Arboretum, 1, 121–146.
Rehder A. 1940. Manual of Cultivated Trees and Shrubs Hardy in North America. Collier Macmillan Ltd.
Purcell S, Neale B M, Toddbrown K, Thomas L, Ferreira M A R, Bender D, Maller J, Sklar P, Bakker P I W D, Daly M J, Sham P C. 2007. PLINK: A tool set for whole-genome association and population-based linkage analyses. American Journal of Human Genetics, 81, 559–575.
Schneider C K. 1906. Illustriertes handbuch der laubholzkunde. Nature, 73, 199.
Wu J, Wang Z W, Shi Z B, Zhang S, Ming R, Zhu S L, Khan M A, et al. 2013. The genome of the pear (Pyrus bretschneideri Rehd.). Genome Research, 23, 396–408.
Yu D J. 1984. Deciduous Fruit Tree Taxonomy. Shanghai Science and Technology Press, China.
Yu D J, Lu L D, Gu C Z, Guan K J, Li C L, Chen S Y. 1987. Volume of Chinese Flora, 36, 37, 38. Science Press, China.
Zabel H 1903. Malus Mill. In: Beissner L, Schelle E, Zabel H eds., Handbuch der Laubholz-Benennung, vol. 189. Berlin: Paul Parey. 3. (in German)
[1] LIU Yu-song, WANG Hong-ying, ZHAO Yong-juan, JIN Yi-bo, LI Chao, MA Feng-wang. Establishment of an efficient regeneration and genetic transformation system for Malus prunifolia Borkh. ‘Fupingqiuzi’[J]. >Journal of Integrative Agriculture, 2022, 21(9): 2615-2627.
[2] ZHANG Zhi-jun, ZHANG Jing, TANG Zhong-wen, WANG Yan-peng, GAO Teng-teng, LIU Xiao-min, MA Feng-wang, LI Chao. Tissue distribution and changes in dopamine during development and stress responses in Malus germplasm[J]. >Journal of Integrative Agriculture, 2022, 21(3): 710-724.
No Suggested Reading articles found!