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| Transgenic restorer rice line T1c-19 with stacked cry1C*/bar genes has low weediness potential without selection pressure |
| HUANG Yao*, LI Ji-kun*, QIANG Sheng, DAI Wei-min, SONG Xiao-ling
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| Weed Research Lab, Nanjing Agricultural University, Nanjing 210095, P.R.China |
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Abstract Stacked (insect and herbicide resistant) transgenic rice T1c-19 with cry1C*/bar genes, its receptor rice Minghui 63 (herein MH63) and a local two-line hybrid indica rice Fengliangyou Xiang 1 (used as a control) were compared for agronomic performance under field conditions without the relevant selection pressures. Agronomic traits (plant height, tiller number, and aboveground dry biomass), reproductive ability (pollen viability, panicle length, and filled grain number of main panicles, seed set, and grain yield), and weediness characteristics (seed shattering, seed overwintering ability, and volunteer seedling recruitment) were used to assess the potential weediness without selection pressure of stacked transgene rice T1c-19. In wet direct-seeded and transplanted rice fields, T1c-19 and its receptor MH63 performed similarly regarding vegetative growth and reproductive ability, but both of them were significantly inferior to the control. T1c-19 did not display weed characteristics; it had weak overwintering ability, low seed shattering and failed to establish volunteers. Exogenous insect and herbicide resistance genes did not confer competitive advantage to transgenic rice T1c-19 grown in the field without the relevant selection pressures.
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Received: 31 July 2015
Accepted:
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| Fund: This research was ?nancially supported by the China Transgenic Organism Research and Commercialization Project (2016ZX08011-001), the National Natural Science Fund Project (31270579), the Specialized Research Fund for the Doctoral Program of Higher Education, China (20130097130006) and the 111 Project of China (B07030). |
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Corresponding Authors:
SONG Xiao-ling, Tel/Fax: +86-25-84395117, E-mail: sxl@njau.edu.cn
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| About author: HUANG Yao, E-mail: huangyaozhiyuan@163.com
* These authors contributed equally to this study. |
Cite this article:
HUANG Yao, LI Ji-kun, QIANG Sheng, DAI Wei-min, SONG Xiao-ling.
2016.
Transgenic restorer rice line T1c-19 with stacked cry1C*/bar genes has low weediness potential without selection pressure. Journal of Integrative Agriculture, 15(05): 1046-1058.
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| Alizadeh M R, Allameh A. 2013. Evaluating rice losses in various harvesting practices. International Research Journal of Applied and Basic Sciences, 4, 894–901.Andow D A, Zwahlen C. 2006. Assessing environmental risks of transgenic plants. Ecology Letters, 9, 196–214.Azhiri-Sigari T, Gines H, Ssebastian L, Wade L. 2005. Seedling vigor of rice cultivars in response to seeding depth & soil moisture. Philippine Journal of Crop Science, 30, 53–58.Baker H G. 1974. The evolution of weeds. Annual Review of Ecology Systematics, 5, 1–24.Cao S, He X, Xu W. 2012. Safety assessment of transgenic Bacillus thuringiensis rice T1c-19 in Sprague-Dawley rats from metabonomics and bacterial profile perspectives. International Union of Biochemistry and Molecular Biology, 64, 242–250.Caton B P, Cope A E, Mortimer M. 2003. Growth traits of diverse rice cultivars under severe competition: Implications for screening for competitiveness. Field Crops Research, 83, 157–172.Caton B P, Foin T C, Hill J E. 1999. A plant growth model for integrated weed management in direct-seeded rice. III. Interspecific competition for light. Field Crops Research, 63, 47–61.Chen L J, Lee D S, Song Z P, Suh H S, Lu B R. 2004. Gene flow from cultivated rice (Oryza sativa) to its weedy and wild relatives. Annals of Botany (London), 93, 67–73.Chun Y J, Kim D I, Park K W, Kim H J, Jeong S C, An J H, Cho K H, Back K, Kim H M, Kim C G. 2011. Gene flow from herbicide-tolerant GM rice and the heterosis of GM rice-weed F2 progeny. Planta, 233, 807–815.Conner A J, Glare T R, Nap J P. 2003. The release of genetically modified crops into the environment. The Plant Journal, 33, 19–46.Cousens R D, Barnett A G, Barry G C. 2003a. Dynamics of competition between wheat and oat: I. Effects of changing the timing of phenological events. Agronomy Journal, 95, 1295–1304.Cousens R D, Rebetzke G J, Barnett A G. 2003b. Dynamics of competition between wheat and oat: II. Effects of dwarfing genes. Agronomy Journal, 95, 1305–1313.Crawley M J, Brown S L, Hails R S, Kohn D D, Rees M. 2001. Transgenic crops in natural habitats. Nature, 409, 682–683.Cudney D W, Jordan L S, Hall A E. 1991. Effect of wild oat (Avena fatua) infestations on light interception and growth-rate of wheat (Triticum aestivum). Weed Science, 39, 175–179.Cui R R, Wei Y, Meng P P, Ma Y L, Li Q R, Dai W M, Song X L. 2012. Assessment on potential weediness of transgenic glufosinate-resistant rice Minghui 86B. Chinese Journal Rice Science, 26, 467–475. (in Chinese)Dong G C, Wang Y, Yu X F, Zhou J, Peng B, Li J Q, Tian H, Zhang Y, Yuan Q M, Wang Y L. 2011. Differences of nitrogen uptake and utilization of conventional rice varieties with different growth duration. Scientia Agricultura Sinica, 44, 4570–4582. (in Chinese)Du B, Chen L G, Zhao T F, Cao X L, Zhang P, Zhang J, Shen Q L, Yuan X M. 2012. Effects of direct-seeding date on growth period and grain yield of different genotypes of rice. Hunan Agricultural Sciences, 15, 18–21. (in Chinese)Eastick R J, Hearnden M N. 2009. Potential for weediness of Bt cotton in northern Australia. Weed Research, 54, 1142–1151. Fang Y, Liu L, Xu B C, Li F M. 2011. The relationship between competitive ability and yield stability in an old and a modern winter wheat cultivar. Plant Soil, 347, 7–23.Guèritaine G, Sester M, Eber F, Chèvre A M, Darmency H. 2002. Fitness of backcross six of hybrids between transgenic oilseed rape (Brassica napus) and wild radish (Raphanus raphanistrum). Molecular Ecology, 11, 1419–1426. Gu X Y, Turnipseed E B, Foley M. 2008. The qSD12 locus controls offspring tissue-imposed seed dormancy in rice. Genetics, 179, 2263–2273.Hovick S M, Campbell L G, Snow A A, Whitney K D. 2012. Hybridization alters early life-history traits and increases plant colonization success in a novel region. The Ametican Naturalist, 179, 192–203.Huang J K, Rozelle S, Pray C, Wang Q F. 2002. Plant biotechnology in China. Science, 295, 674–677.Huang Y, Li J K, Qiang S, Luo T P, Song X L. 2015. Gene flow from stacking of transgenic insect and glufosinate-resistant rice T1c-19 to weedy and cultivated rice. Chinese Journal of Applied and Environmental Biology, 21, doi: 10.3724/SP.J.1145.2015.05030 (in Chinese)Huang Y, Wang J, Dai W M, Qiang S, Song X L. 2014. The risk assessment of potential weediness of gene stacked rice B2A68 in Nanjing area. Chinese Weed Science, 32, 60–68. (in Chinese)IRRI (International Rice Research Institute). 2002. Standard Evaluation System for Rice. International Rice Research Institute, Los Banos, Manila, Philippines. James C. 2014. Global status of commercialized biotech/GM crops: 2014. [2014-10-15]. http://www.isaaa.org/resources/publications/briefs/49/toptenfacts/default.aspJia S R, Peng Y F. 2002. GMO biosafety research in China. Environmental Biosafety Research, 1, 5–8.Jiang S Y, Ramachandran S. 2010. Functional genomics of rice pollen and seed development by genome-wide transcript profiling and Ds insertion mutagenesis. International Journal of Biological Sciences, 7, 28–40.Kawata M, Murakami K, Ishikawa T. 2009. Dispersal and persistence of genetically modified oilseed rape around Japanese harbors. Environmental Science and Pollution Research, 16, 120–126.Kim S, Kim C, Li W, Kim T, Li W, Zaidi M A. 2008. Inheritance and field performance of transgenic Korean Bt rice lines resistant to rice yellow stem borer. Euphytica, 164, 829–839.Knispel A L, McLachlan S M. 2010. Landscape-scale distribution and persistence of genetically modified oilseed rape (Brassica napus) in Manitoba, Canada. Environmental Science and Pollution Research, 17, 13–25.Knispel A L, McLachlan S M, Van Acker R C, Friesen L F. 2008. Gene flow and multiple herbicide resistance in escaped canola populations. Weed Science, 56, 72–80.Konishi S, Izawa T, Lin S Y, Ebana K, Fukuta Y, Sasaki T, Yano M. 2006. An SNP caused loss of seed shattering during rice domestication. Science, 312, 1392–1396.Li X Y, Qian Q, Fu Z M, Wang Y H, Xiong G S, Zeng D L, Wang X Q, Liu X F, Teng S, Hiroshi F, Yuan M, Luo D, Han B, Li J Y. 2003. Control of tillering in rice. Nature, 422, 618–621.Liu Y B, Ge F, Liang Y Y, Wu G, Li J S. 2015. Characterization of competitive interactions in the coexistence of Bt-transgenic and conventional rice. BMC Biotechnology, 15, 27.Londo J P, Bautista N S, Sagers C L, Lee E H, Watrud L S. 2010. Glyphosate drift promotes changes in fitness and transgene gene flow in canola (Brassica napus) and hybrids. Annual Botany, 106, 957–965.Lu Z B, Tian J C, Han N S, Hu C, Peng Y F, Stanley D, Ye G Y. 2014. No direct effects of two transgenic Bt rice lines, T1C-19 and T2A-1, on the arthropod communities. Environmental Entomolology, 43, 1453–1463.Martinkova Z, Honek A. 2011. Asymmetrical intraspecific competition in Echinochloa crus-galli is related to differences in the timing of seedling emergence and seedling vigour. Plant Ecology, 212, 1831–1839.Mason H E, Navabi A, Frick B L, O’Donovan J T, Spaner D M. 2007. The weed-competitive ability of Canada western red spring wheat cultivars grown under organic management. Crop Science, 47, 1167–1176Mercer K L, Andow D A, Wyse D L, Shaw R G. 2007. Stress and domestication traits increase the relative fitness of crop-wild hybrids in sunflower. Ecology Letters, 10, 383–393.Messeguer J, Marfa V, Catala M M, Guiderdoni E, Mele E A. 2004. Field study of pollen-mediated gene flow from Mediterranean GM rice to conventional rice and the red rice weed. Molecular Breeding, 13, 103–112.Muenscher W C. 1980. Weeds. 2nd ed. Cornell University Press, Ithaca and London. p. 586.Munier D J, Brittan K L, Lanini W T. 2012. Seed bank persistence of genetically modified canola in California. Environmental Science and Pollution Research, 19, 2281–2284.Olguin E R, Arrieta-Espinoza G, Lobo J A, Espinoza-Esquivel A M. 2009. Assessment of gene flow from a herbicide-resistant indica rice (Oryza sativa L.) to the Costa Rican weedy rice (Oryza sativa) in tropical America: Factors affecting hybridization rates and characterization of F1 hybrids. Transgenic Research, 18, 633–647.Parker I M, Kareiva P. 1996. Assessing the risks of invasion for genetically engineered plants: Acceptable evidence and reasonable doubt. Biological Conservation, 78, 193–203.Purrington C B, Bergelson J. 1995. Assessing weediness of transgenic crops: Industry plays plant ecologist. Trends in Ecology & Evolution, 10, 340–342.Rajala A, Niskanen M, Isolahti M, Peltonen-sainio P. 2011. Environmental factors associated with depth of the seed in the soil and soil characteristics themselves as well as the crop’s intrinsic germination rate determine seedling emergence. Agricultural and Food Science, 20, 228–234.Roberts A, Devos Y, Raybould A, Bigelow P, Gray A. 2014. Environmental risk assessment of GE plants under low-exposure conditions. Transgenic Research, 23, 971–983.Rong J, Lu B R, Song Z P, Su J, Snow A A, Zhang X S, Sun S G, Chen R, Wang F. 2006. Dramatic reduction of crop-to-crop gene flow within a short distance from transgenic rice fields. New Phytologist, 173, 346–353.Rose C W, Millwood R J, Moon H S, Rao M R, Halfhill M D, Raymer P L, Warwick4 S I, Al-Ahmad H, Gressel J, Stewart Jr C N. 2009. Genetic load and transgenic mitigating genes in transgenic Brassica rapa (field mustard)×Brassica napus (oilseed rape) hybrid populations. BMC Biotechnology, 9, 93.Saji H, Nakajima N, Aono M, Tamaoki M, Kubo A, Wakiyama S. 2005. Monitoring the escape of transgenic oilseed rape around Japanese ports and roadsides. Environmental Biosafety Research, 4, 217–222.Schafer M G, Ross A A, Londo J P, Burdick C A, Lee E H, Travers S E, Van de Water P K, Sagers C L. 2011. The Establishment of genetically engineered canola populations in the U.S. PLoS ONE, 6, e25736.Simard M J, Légère A, Séguin-Swartz G, Nair H, Warwick S. 2005. Fitness of double vs. single herbicide resistant canola. Weed Science, 53, 489–498.Singla-Pareek S L, Reddy M K, Sopory S K. 2003. Genetic engineering of the glyoxalase pathway in tobacco leads to enhanced salinity tolerance. Proceedings of the National Academy of Sciences of the United States of America, 100, 14672–14677.Song X L, Qiang S, Peng Y F. 2009. An experimental case of safety assessment of weediness of transgenic glyphosate-resistant soybean (Glycine mac (L.) Merri). Scinetia Agricultura Sinica, 42, 145–153. (in Chinese)Song X L, Wang Z, Qiang S. 2011. Agronomic performance of F1, F2 and F3 hybrids between weedy rice and transgenic glufosinate-resistant rice. Pest Management Science, 67, 921–931.Sun G H, Dai W M, Cui R R, Qiang S, Song X L. 2015. Gene flow from glufosinate-resistant transgenic hybrid rice Xiang 125S/Bar68-1 to weedy rice and cultivated rice under different experimental designs. Euphytica, 204, 1–17.Tang W, Chen H, Xu C G, Li X H, Lin Y J, Zhang Q F. 2006. Development of insect-resistant transgenic indica rice with a synthetic cry1C* gene. Molecular Breeding, 18, 1–10. Warwick S I, Stewart C N. 2005. Crops come from wild plants - How domestication, transgenes, and linkage together shape ferality. In: Crop Ferality and Volunteerism, Chapter 2. pp. 9–30.West A M, Richardson R J, Arellano C, Burton M G. 2010. Bushkiller (Cayratia japonica) growth in interspecific and intraspecific competition. Weed Science, 58, 195–198.De Wet J M J, Harlan J R. 1975. Weeds and domesticates: evolution in the man-made habitat. Economic Botany, 29, 99–107.Wolt J D. 2009. Advancing environmental risk assessment for transgenic biofeedstock crops. Biotechnology for Biofuels, 2, 27.Xia H, Chen L Y, Wang F, Lu B R. 2010. Yield benefit and underlying cost of insect-resistance transgenic rice: Implication in breeding and deploying transgenic crops．Field Crops Research, 118, 215–220.Xia H, Lu B R, Xu K, Wang W, Yang X, Yang C, Luo J, Lai F, Ye W, Fu Q. 2011. Enhanced yield performance of Bt rice under target-insect attacks: implications for field insect management. Transgenic Research, 20, 655–664.Yang X, Wang F, Su J, Lu B R. 2012. Limited fitness advantages of crop-weed hybrid progeny containing insect-resistant transgenes (Bt/CpTI) in transgenic rice field. PLoS ONE, 7, e41220. Yang X, Xia H, Wang W, Wang F, Su J, Snow A A, Lu B R. 2011. Transgenes for insect resistance reduce herbivory and enhance fecundity in advanced generations of crop-weed hybrids of rice. Evolutionary Applications, 4, 672–684.Yoshimura Y, Beckie H J, Matsuo K. 2006. Transgenic oilseed rape along transportation routes and port of Vancouver in western Canada. Environmental Biosafety Research, 5, 67–75.Yuan L P. 2002. Science of Hybrid Rice. China Agriculture Press, Beijing. (in Chinese)Zhang J X, Lu Z M, Dai W M, Song X L, Peng Y F, Valverde B E, Qiang S. 2015. Cytoplasmic-genetic male sterility gene provides direct evidence for some hybrid rice recently evolving into weedy rice. Scientific Reports, 5, 10591.Zhang W Q, Linscombe S D, Webster E, Tan S Y, Oard J. 2006. Risk assessment of the transfer of imazethapyr herbicide tolerance from clear?eld rice to red rice (Oryza sativa). Euphytica, 152, 75–86Zhang X, Yang Y, Xu H, Chen H, Wang B, Lin Y, Lu Z. 2011. Resistance performances of transgenic Bt rice lines T(2A)-1 and T1c-19 against Cnaphalocrocis medinalis (Lepidoptera: Pyralidae). Journal of Economic Entomology, 104, 1730–1735.Zhou H Y, Gao Q B, Chen X F. 2010. Studies on the outcrossing characteristics of the parents of mid-cropping super hybrid rice Fengliangyou Xiang 1. Hybrid Rice, S1, 502–505. (in Chinese)Zhou Y, Lu D F, Li C Y, Luo J H, Zhu B F, Zhu J J, Shangguan Y Y, Wang Z X, Sang T, Zhou B, Han B. 2012. Genetic control of seed shattering in rice by the APETALA2 transcription factor SHATTERING ABORTION1. The Plant Cell, 24, 1034–1048. Zuo J, Zhang L J, Song X L, Dai W M, Qiang S. 2011. Innate factors causing differences in gene ?ow frequency from transgenic rice to different weedy rice biotypes. Pest Management Science, 67, 677–690. |
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