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Journal of Integrative Agriculture  2019, Vol. 18 Issue (1): 115-123    DOI: 10.1016/S2095-3119(18)62104-5
Special Issue: 植物细菌真菌合辑Plant Bacteria/Fungus
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Effect of plant extracts on activity of some defense enzymes of apple fruit in interaction with Botrytis cinerea
Jalal Gholamnezhad
Department of Horticultural Sciences, Faculty of Agriculture & Natural Resources, Ardakan University, P.O. Box 184, Ardakan 8951895491, Iran
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The efficacy of seven plant extracts (neem, fennel, lavender, thyme, pennyroyal, salvia and asafetida) in controlling postharvest of apple (caused by Botrytis cinerea) was evaluated in vitro and in vivoIn vitro, all plant extracts treatments inhibited spore germination.  Inhibitory rates of pore germination was 17.41 and 20.83% for neem extract treatment (methanolic and aqueous extracts, respectively) with significant difference compared to control (73.6 and 85.33%) for aqueous and methanol extracts.  In the storage conditions, the application of aqueous extract of neem (at concentration of 25%) resulted in 89.11% reduction of disease severity compared with the untreated control.  Results of enzymes activity showed the plant extracts can increase the activity of peroxidase, phenylalanine ammonia-lyase, β-1,3-glucanase and polyphenol oxidase in the presence of pathogens, in apple fruits.  However, the results of this research revealed that application of neem extracts was more effective than the application of other plant extracts.  According to this study, it could be concluded that plant extracts may be useful to control postharvest disease as a safe alternative option to chemical fungicides. 
Keywords:  Botrytis cinerea        defense enzymes        plant extracts        spore germination
Received: 05 February 2018   Accepted:
Corresponding Authors:  Correspondence Jalal Gholamnezhad, Tel: +98-353-5252254, Fax: +98-353-2226767, E-mail:,    

Cite this article: 

Jalal Gholamnezhad. 2019. Effect of plant extracts on activity of some defense enzymes of apple fruit in interaction with Botrytis cinerea. Journal of Integrative Agriculture, 18(1): 115-123.

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