|
|
|
|
|
| A method to evaluate the bioactive function of fruit extracts of Chinese wild Citrus with microtubular activity |
| GUAN Xin1, 4*, TAN Si1, 2*, Günther Buchholz3, Peter Nick2, ZHOU Zhi-qin1, 5 |
1 College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, P.R.China
2 Molecular Cell Biology, Botanical Institute, Karlsruhe Institute of Technology, Karlsruhe D-76128, Germany
3 RLP AgroScience/AlPlanta-Institute for Plant Research, Neustadt an der Weinstrasse D-67435, Germany
4 Laboratoire Vigne Biotechnologies et Environnement EA-3991, Université de Haute-Alsace, Colmar 68000, France
5 Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400716, P.R.China |
|
|
|
|
Abstract China is one of the most important centers of origin for Citrus genetic resources. Due to the high content of secondary metabolites, mining wild Chinese Citrus for novel medical applications is promising. In this study, extracts of Chinese wild species from different taxonomical groups were screened for potential effects on microtubules (MTs) in vitro. MT density as a readout for nucleation, and frequency distribution over MT lengths as a readout for elongation and decay were determined by quantitative image analysis via a standardized coverslip assay using fluorescently labelled neurotubulin. Extract from peels of Citrus ichangensis Swing. strongly increased the density of MTs; whereas, extract from peels of Citurs limon (L.) Burm.f. exerted the opposite effect. Extract from pulp of Citrus limonia Osbeck promoted MT elongation, and in addition induced a small population of very long MTs. These data suggest that wild Chinese Citrus harbour compounds that act specifically on different aspects of MT nucleation, elongation, and decay.
|
|
Received: 19 May 2016
Accepted:
|
| Fund: This work was supported by the Fundamental Research Funds for the Central Universities, China (SWU113107 and XDJK2014A014), the Programs for Research on the Teaching Reform of Graduate Education and Basic Science and Cutting-edge Technology Research (yjg153078 and cstc2016jcyjA0489) in Chongqing, China. A fellowship of Chinese “111” project (B12006) to Mr. Tan Si in lab of Prof. Peter Nick, Molecular Cell Biology, Botanical Institute, Karlsruhe Institute of Technology, Germany. |
|
Corresponding Authors:
GUAN Xin, E-mail: xinguan.cnde@googlemail.com; ZHOU Zhi-qin, Tel: +86-23-68250229, Fax: +86-23-68251274, E-mail: zhouzhiqin@swu.edu.cn
|
Cite this article:
GUAN Xin, TAN Si, Günther Buchholz, Peter Nick, ZHOU Zhi-qin.
2017.
A method to evaluate the bioactive function of fruit extracts of Chinese wild Citrus with microtubular activity. Journal of Integrative Agriculture, 16(04): 867-873.
|
| Akhmanova A, Steinmetz M O. 2008. Tracking the ends: A dynamic protein network controls the fate of microtubule tips. Nature Reviews Molecular Cell Biology, 9, 309–322.del Alonso A C, Grundke-Iqbal I, Barra H S, Iqbal K. 1997. Neurobiology abnormal phosphorylation of tau and the mechanism of alzheimer neurofibrillary degeneration: Sequestration of microtubule-associated proteins 1 and 2 and the disassembly of microtubules by the abnormal tau. Proceedings of the National Academy of Sciences of the United States of America, 94, 298–303.Altshuler O, Abu-Abied M, Chaimovitsh D, Shechter A, Frucht H, Dudai N, Sadot E. 2013. Enantioselective effects of (+)- and (-)-citronellal on animal and plant microtubules. Journal of Natural Products, 76, 1598–1604.Bloomquist J R 1996. Ion channels as targets for insecticides. Annual Review of Entomology, 41, 163–190.Brown C R, Doxsey S J, Hongbrown L O, Martin R L, Welch W J. 1996. Molecular chaperones and the centrosome - a role for TCP-1 in microtubule nucleation. Journal of Biological Chemistry, 271, 824–832Chaimovitsh D, Abu-Abied M, Belausov E, Rubin B, Dudai N, Sadot E. 2010. Microtubules are an intracellular target of the plant terpene citral. Plant Journal, 61, 399–408.Ding X, Guo L, Zhang Y, Fan S, Gu M, Lu Y, Jiang D, Li Y, Huang C, Zhou Z. 2013. Extracts of pomelo peels prevent high-fat diet-induced metabolic disorders in C57BL/6 mice through activating the PPARα and GLUT4 pathway. PLOS ONE, 8, e77915.Dostál V, Libusová L. 2014. Microtubule drugs: action, selectivity, and resistance across the kingdoms of life. Protoplasma, 251, 991–1005.Gmitter Jr F G, Hu X. 1990. The possible role of Yunnan, China, in the origin of contemporary Citrus species (Rutaceae): Economic Botany, 44, 267–277.Goodsell D S. 2000. The molecular perspective: Microtubules and the taxanes. The Oncologist, 5, 345–346.Himmelspach R, Nick P, Schäfer E, Ehmann B. 1997. Developmental and light-dependent changes of the cytosolic chaperonin containing TCP-1 (CCT) subunits in maize seedlings, and the localization in coleoptiles. The Plant Journal, 12, 1299–1310.Howard J, Hyman A A. 2003. Dynamics and mechanics of the microtubule plus end. Nature, 422, 753–758.Jia M, Li X. 2005. Chinese Traditional Medicine Records of Ethnic Minorities. China Medical Science Press, Beijing. (in Chinese) Job D, Valiron O, Oakley B. 2003. Microtubule nucleation. Current Opinion in Cell Biology, 15, 111–117.Kollman J M, Merdes A, Mourey L, Agard D A. 2011. Microtubule nucleation by γ-tubulin complexes. Nature Reviews Molecular Cell Biology, 12, 709–721. Li J W H, Vederas J C. 2009. Drug discovery and natural products: end of an era or an endless frontier? Science, 325, 161–165.Lodish H, Berk A, Zipursky S L, Matsudaira P, Baltimore D, James D. 2000. Microtubule dynamics and motor proteins during mitosis. In: Molecular Cell Biology. Section 19.5. 4th ed. H W Freeman and Company, New York. [2016-8-12]. Available from: https://www.ncbi.nlm.nih.gov/books/NBK21537/Lu Y, Xi W, Ding X, Fan S, Zhang Y, Jiang D, Li Y, Huang C, Zhou Z. 2013. Citrange fruit extracts alleviate obesity-associated metabolic disorder in high-fat diet-induced obese C57BL/6 mouse. International Journal of Molecular Sciences, 14, 23736–23750.Margolis R L, Wilson L. 1977. Addition of colchicine-tubulin complex to microtubule ends: The mechanism of substoichiometric colchicine poisoning. Proceedings of the National Academy of Sciences of the United States of America, 74, 3466–3470.Martin S R, Butler F M M, Clark D C, Zhou J M, Bayley P M. 1987. Magnesium ion effects on microtubule nucleation in vitro. Biochimica et Biophysica Acta, 914, 96–100.Mitchison T, Kirschner M. 1984. Microtubule assembly nucleated by isolated centrosomes. Nature, 312, 232–242.Owellen R J, Hartke C A, Dickerson R M, Hains F O. 1976. Inhibition of tubulin-microtubule polymerization by drugs of the Vinca alkaloid class. Cancer Research, 36, 1499–1502.Pasqua G, Monacelli B, Valletta A. 2004. Cellular localisation of the anti-cancer drug camptothecin in Camptotheca acuminate Decne (Nyssaceae). European Journal of Histochemistry, 48, 321–328.Popov N, Schmitt S, Matthies H. 1975. Eine störungsfreie mikromethode zur bestimmung des proteingehaltes in gewebehomogenaten. Acta Biologica et Medica Germanica, 34, 1441–1446. (in Germany)Portran D, Gaillard J, Vantard M, Thery M. 2013. Quantification of MAP and molecular motor activities on geometrically controlled microtubule networks. Cytoskeleton, 70, 12–23.Qiao F, Chong H, Wang R, Yin J, Qian D, Yang X, Jiang X, Nick P. 2014. De-novo characterization of a Cephalotaxus hainanensis transcriptome and genes related to paclitaxel biosynthesis. PLOS ONE, 9, e106900.Rattan R S. 2010. Mechanism of action of insecticidal secondary metabolites of plant origin. Crop Protection, 29, 913–920.Sadot E. 2014. Plant Compounds acting on the cytoskeleton. In: Nick P, Opatrny Z, eds., Applied Plant Cell Biology. Springer, Berlin Heidelber. pp. 301–323.Saito K, Matsuda F. 2010. Metabolomics for functional genomics, systems biology, and biotechnology. Annual Review of Plant Biology, 61, 463–489.Shelanski M L, Gaskin F, Cantor C R. 1973. Microtubule assembly in the absence of added nucleotides. Proceedings of the National Academy of Sciences of the United States of America, 70, 765–767.Subramanian R, Wilson-Kubalek E M, Arthur C P, Bick M J, Campbell E A, Darst S A, Milligan R A, Kapoor T M. 2010. Insights into antiparallel microtubule crosslinking by PRC1, a conserved nonmotor microtubule binding protein. Cell, 142, 433–443.Sun J. 2007. D-Limonene: Safety and clinical applications. Alternative Medicine Review, 12, 259–264.Tan S, Li M, Ding X, Fan S, Guo L, Gu M, Zhang Y, Feng L, Jiang D, Li Y, Xi W, Huang C, Zhou Z. 2014. Effects of fortunella margarita fruit extract on metabolic disorders in high-fat diet-induced Obese C57BL/6 mice. PLOS ONE, 9, e93510.Touil Y S, Fellous A, Scherman D, Chabot G G. 2009. Flavonoid-induced morphological modifications of endothelial cells through microtubule stabilization. Nutrition and Cancer, 61, 310–321.Wink M. 2000. Interference of alkaloids with neuroreceptors and ion channels. Studies in Natural Products Chemistry, 21, 3–122.Xi W, Fang B, Zhao Q, Jiao B, Zhou Z. 2014a. Flavonoid composition and antioxidant activities of Chinese local pummelo (Citrus grandis Osbeck.) varieties. Food Chemistry, 161, 230–238.Xi W, Zhang Y, Sun Y, Shen Y, Ye X, Zhou Z. 2014b. Phenolic composition of Chinese wild mandarin (Citrus reticulata Balnco.) pulps and their antioxidant properties. Industrial Crops and Products, 52, 466–474.Xue X Y, Liao M J, Lin L F, Zhang Z, Zhou X W, Zhou X, Luo H M. 2012. Phosphorylation of Akt is involved in protocatechuic acid-induced neurotrophic activity. Neurological Research, 34, 901–907.Zhang Y, Sun Y, Xi W, Shen Y, Qiao L, Zhong L, Ye X, Zhou Z. 2014. Phenolic compositions and antioxidant capacities of Chinese wild mandarin (Citrus reticulata Blanco) fruits. Food Chemistry, 145, 674–680.Zhou K, Ye M. 2010. China Fruit Records: Citrus Volume. China Forestry Press, Beijing. pp. 62–130. (in Chinese) |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
