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Journal of Integrative Agriculture  2017, Vol. 16 Issue (01): 125-134    DOI: 10.1016/S2095-3119(16)61452-1
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Alleles contributing to acetyl coenzyme A carboxylase (ACCase) resistance in keng stiffgrass (Pseudosclerochloa kengiana) populations from China
YUAN Guo-hui1, GUO Wen-lei1, DU Long1, LIU Wei-tang1, LI Qi1, LI Ling-xu2, WANG Jin-xin1

1 College of Plant Protection, Shandong Agricultural University, Tai’an 271018, P.R.China

2 College of Chemistry and Pharmacy Science, Qingdao Agricultural University, Qingdao 266109, P.R.China

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Abstract  Keng stiffgrass is a grass weed that affects wheat-rice cropping systems in China.  The extensive reliance on acetyl coenzyme A carboxylase (ACCase)-inhibiting herbicides has resulted in keng stiffgrass developing resistance to these herbicides.  The objective of this research was to evaluate the resistance level of the putative resistant keng stiffgrass populations to ACCase-inhibiting herbicides and to identify their molecular resistance mechanism.  Whole-plant dose-response experiments demonstrated that SD-4 (R), SD-11 (R), and JS-25 (R) populations were highly resistant to fenoxaprop, clodinafop, and fluazifop, moderately resistant to diclofop, had low resistance to sethoxydim and pinoxaden, but were sensitive to clethodim.  Partial chloroplastic ACCase sequences showed that there were two copies of ACCase gene in keng stiffgrass, and all homoeologous genes were expressed.  The results of sequence analyses of the ACCase CT domain revealed an isoleucine-to-asparagine substitution at position 2041 in SD-4 (R) and SD-11 (R) populations, and a tryptophan-to-cysteine substitution at position 2027 in the JS-25 (R) population.  To our knowledge, this is the first report of Ile-2041-Asn and Trp-2027-Cys mutations in ACCase-resistant keng stiffgrass.  In addition, three robust (derived) cleaved amplified polymorphic sequence ((d)CAPS) markers have been developed to rapidly identify these mutations in the ACCase gene of keng stiffgrass.
Keywords:  herbicide resistance      ACCase gene      mutation      (d)CAPS  
Received: 25 March 2016   Accepted:

This research was financially supported by the Special Fund for Agro-scientific Research in the Public Interest, China (201303031) and the National Natural Science Foundation of China (31471787).

Corresponding Authors:  WANG Jin-xin, Tel: +86-538-8241114, E-mail:    
About author:  YUAN Guo-hui, E-mail:

Cite this article: 

YUAN Guo-hui, GUO Wen-lei, DU Long, LIU Wei-tang, LI Qi, LI Ling-xu, WANG Jin-xin. 2017. Alleles contributing to acetyl coenzyme A carboxylase (ACCase) resistance in keng stiffgrass (Pseudosclerochloa kengiana) populations from China. Journal of Integrative Agriculture, 16(01): 125-134.

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