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Journal of Integrative Agriculture  2016, Vol. 15 Issue (7): 1432-1440    DOI: 10.1016/S2095-3119(15)61165-0
Crop Genetics · Breeding · Germplasm Resources Advanced Online Publication | Current Issue | Archive | Adv Search |
Pyramiding blast, bacterial blight and brown planthopper resistance genes in rice restorer lines
JI Zhi-juan1, 2*, Yang Shu-dong3*, ZENG Yu-xiang2, LIANG Yan2, YANG Chang-deng2, QIAN Qian1, 2
1 College of Agronomy, Shenyang Agricultural University, Shenyang 110866, P.R.China 
2 State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, P.R.China
3 Shengzhou Seed Production Farm, Shaoxing 312000, P.R.China
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Abstract       Rice blast, bacterial blight (BB) and brown planthopper (BPH) are the three main pests of rice. This study investigated pyramiding genes resistant to blast, BB and BPH to develop restorer lines. Ten new lines with blast, BB and/or BPH resistance genes were developed using marker-assisted selection (MAS) technique and agronomic trait selection (ATS) method. Only HR13 with resistance genes to blast, BB and BPH was obtained. In addition to blast and BB resistance, four lines (HR39, HR41, HR42, HR43) demonstrated moderate resistance to BPH, but MAS for BPH resistance genes were not conducted in developing these four lines. These data suggested that there were unknown elite BPH resistance genes in the Zhongzu 14 donor parent. A more effective defense was demonstrated in the lines with Pi1 and Pi2 genes although the weather in 2012 was favorable to disease incidence. Blast resistance of the lines with a single resistance gene, Pita, was easily influenced by the weather. Overall, the information obtained through pyramiding multiple resistance genes on developing the restorer lines is helpful for rice resistance breeding.
Keywords:  rice        blast        bacterial blight        brown planthopper        resistance        pyramid  
Received: 25 May 2015   Accepted:
Fund: 

This work was financially supported by the National Natural Science Foundation of China (31221004), the Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences, the Science and Technology Program of Zhejiang Province, China (2015C32056) and the Research Project on Public Welfare from the Ministry of Agriculture of China (2012RG001-5).

Corresponding Authors:  YANG Chang-deng, Tel/Fax: +86-571-63370367, E-mail: yangchangdeng@126.com; QIAN Qian, Tel/Fax: +86-571-63371418, E-mail: qianqian188@hotmail.com   

Cite this article: 

JI Zhi-juan, Yang Shu-dong, ZENG Yu-xiang, LIANG Yan, YANG Chang-deng, QIAN Qian. 2016. Pyramiding blast, bacterial blight and brown planthopper resistance genes in rice restorer lines. Journal of Integrative Agriculture, 15(7): 1432-1440.

Amante-bordeos A, Sitch L A, Nelson R, Dalmacio R D, Oliva N P, Aswinnor H, Leung H. 1992. Transfer of bacterial-blight and blast resistance from the tetraploid wild rice Oryza minuta to cultivated rice, Oryza sativa. Theoretical and Applied Genetics, 84, 345–354.

Blair M W, Garris A J, Iyer A S, Brad C, Stephen K, McCouch S R. 2003. High resolution genetic mapping and candidate gene identification at the xa5 locus for bacterial blight resistance in rice (Oryza sativa L.). Theoretical and Applied Genetics, 107, 62–73.

Bosque-Perez N A, Buddenhagen I W. 1992. The development of host-plant resistance to insect pests: Outlook for the tropics. In: Menken S B J, Visser J H, Harrewijn P, eds., Proceedings of the 8th International Symposium Insect-Plant Relationships. Kluwer Academic Publishers, Dor-drecht. pp. 235–249.

Chen D H, Zeigler R S, Ahn S W, Nelson R J. 1996. Phenotypic characterization of the rice blast resistance gene Pi-2(t). Plant Disease, 80, 52–56.

Chen H L, Chen B T, Zhang D P, Xie Y F, Zhang Q. 2001. Pathotypes of Pyriculara grisea in rice fields of central and southern China. Plant Disease, 8, 843–850.

Chen S C, Xu G, Lin X H, Zhang Q. 2001. Improving bacterial blight resistance of 6078, an elite restorer line of hybrid rice, by molecular marker-aided selection. Plant Breeding, 120, 133–137.

Chen S X, Lin H, Xu C G, Zhang Q. 2000. Improvement of bacterial blight resistance of ‘Minghui 63’, an elite restorer line of hybrid rice, by molecular marker-assisted selection. Crop Science, 40, 239–244.

Cheng S H, Zhuang J Y, Fan Y Y, Du J H, Cao L Y. 2007. Progress in research and development on hybrid rice: A super-domesticate in China. Annals of Botany, 100, 959–966.

Cheng Y, Gao B D, Chen H Y, Mao J J, Cao A X, Zhu J G, Zhu S F. 2012. Identification of WA-type three-line hybrid rice with real-time polymerase chain reaction (PCR) method. Applied Biochemistry and Biotechnology, 166, 819–829.

Garris A J, McCouch S R, Kresovich S. 2003. Population structure and its effect on haplotype diversity and linkage disequilibrium surrounding the xa5 locus of rice (Oryza sativa L.). Genetics, 165, 759–769.

Heinrichs E A. 1986. Perspectives and directions for the continued development of insect-resistant rice varieties. Agriculture, Ecosystems and Environment, 18, 9–36.

Hittalmani S, Parco A, Mew T V, Zeigler R S, Huang N. 2000. Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice. Theoretical and Applied Genetics, 100, 1121–1128.

Huang N, Angeles E R, Domingo J, Magpantay G, Singh S, Zhang Q, Kumaravadivel N, Bennett J, Khush G S. 1997. Pyramiding of bacterial resistance genes in rice: Marker aided selection using RFLP and PCR. Theoretical and Applied Genetics, 95, 313–320.

IRRI (International Rice Research Institute). 1988. Standard Evaluation Systems for Rice. IRRI, Manila, Philippines.

Jena K K, Jeung J U, Lee J H, Choi H C, Brar D S. 2006. High-resolution mapping of a new brown planthopper (BPH) resistance gene, Bph18(t), and marker-assisted selection for BPH resistance in rice (Oryza sativa L.). Theoretical and Applied Genetics, 112, 288–297.

Ji Z J, Shi J Y, Zeng Y X, Qian Q, Yang C D. 2014. Application of a simplified marker-assisted backcross technique for hybrid breeding in rice. Biologia, 69, 463–468.

Jia Y, Redus M A, Wang Z H, Rutger J N. 2004. Development of a SNLP marker from the Pi-ta blast resistance gene by tri-primer PCR. Euphytica, 138, 97–101.

Jiang G H, Xu C G, Tu J M, Li X H, He Y Q, Zhang Q F. 2004. Pyramiding of insect- and disease-resistance genes into an elite indica, cytoplasm male sterile restorer line of rice, ‘Minghui 63’. Plant Breeding, 123, 112–116.

Jiang H C, Feng Y T, Bao L, Li X, Gao G J, Zhang Q L, Xiao J H, Xu C G, He Y Q. 2012. Improving blast resistance of Jin 23B and its hybrid rice by marker-assisted gene pyramiding. Molecular Breeding, 30, 1679–1688.

 Jirapong J, Kalaya S, Waraporn W, Jate K. 2010. Detection of a brown planthopper resistance gene bph4 at the same chromosomal position of Bph3 using two different genetic backgrounds of rice. Breeding Science, 60, 71–75.

Jirapong J, Kittiphong P, Sanguan T, Apichart V, Theerayut T. 2007. Mapping of a broad-spectrum brown planthopper resistance gene, Bph3, on rice chromosome 6. Molecular Breeding, 19, 35–44.

Johnson R. 1981. Durable disease resistance. In: Jenkyn, J F, Plumb R T, eds., Strategies for the Control of Cereal Diseases. Blackwell Scientific Publications, Oxford. pp. 55–63.

Khush G S, Jena K K. 2009. Current status and future prospects for research on blast resistance in rice (Oryza sativa L.). In: Wang, G L, Valent B, eds., Advances in Genetics, Genomics and Control of Rice Blast Disease. Springer, New York. pp.1–10.

Mackill D J, Bonman J M. 1992. Inheritance of blast resistance in near isogenic lines of rice. Phytopathology, 82, 746–749.

Mundt C C. 1990. Probability of mutation to multiple virulence and durability of resistance gene pyramids. Phytophathology, 80, 221–223.

Peñalver C A, Arida A, Heong K L, Horgan F G. 2011. Aspects of brown planthopper adaptation to resistant rice varieties with the Bph3 gene. Entomologia Experimentalis et Applicata, 141, 245–257.

Sharma P N, Torii A, Takumi S, Mori N, Nakamura C. 2004. Marker-assisted pyramiding of brown planthopper (Nilaparvata lugens Stål) resistance genes Bph1 and Bph2 on rice chromosome 12. Hereditas, 140, 61–69.

Srinivasan B, Gnanamanickam S. 2005. Identification of a new source of resistance in wild rice, Oryza rufipogon to bacterial blight of rice caused by Indian strains of Xanthomonas oryzae pv. Oryzae. Current Science, 88, 25.

Sundaram R M, Vishnupriya M R, Biradar S K, Laha G S, Reddy A G, Rani N S, Sarma N P, Sonti R V. 2008. Marker assisted introgression of bacterial blight resistance in Samba Mahsuri, an elite indica rice variety. Euphytica, 160, 411–422.

Sundaram R M, Vishnupriya M R, Laha G S, Shobha R N, Srinivas R P, Balachandaran S M, Ashok R G, Sarma N P, Shonti R V. 2009. Introduction of bacterial blight resistance into Triguna, a high yielding, mid-early duration rice variety. Biotechnology, 4, 400–407.

Sun L H, Wang C M, Su C C, Liu Y Q, Zhai H Q, Wan J M. 2006. Mapping and marker-assisted selection of a brown planthopper resistance gene bph2 in rice (Oryza sativa L.). Acta Genetica Sinica, 33, 717–723.

Tao R, Chen J, Liao Q. 2006. Field Manual of Diseases and Pests in Rice Identification and Resistance Evaluation for the Diseases and Pests. China Agricultural Science and Technology Press, Beijing. pp. 118–122. (in Chinese)

Wang C, Qi H, Pan H, Li J, Fan Y, Zhang Q, Zhao K. 2005. EST-Markers flanking the rice bacterial blight resistance gene Xa23 and their application in marker-assisted selection. Scientia Agricultura Sinica, 38, 1996–2001. (in Chinese)

Wang X, Fjellstrom R, Jia Y, Yan W G, Jia M H, Scheffler B E, Wu D, Shu Q, McClung A. 2010. Characterization of Pi-ta blast resistance gene in an international rice core collection. Plant Breeding, 129, 491–501.

Wang Z, Jia Y, Rutger J N, Xia Y. 2007. Rapid survey for presence of a blast resistance gene Pi-ta in rice cultivars using the dominant DNA markers derived from portions of the Pi-ta gene. Plant Breeding, 126, 36–42.

Zhang Q, Lin S, Zhao B, Wang C, Yang W, Zhou Y, Li D, Chen C, Zhu L. 1998. Identification and tagging a new gene for resistance to bacterial blight (Xanthomonas oryzae pv. oryzae) from O. rufipogon. Rice Genetics Newsletter, 15, 138–142.

Zhang Q, Wang C, Zhao K, Zhao Y, Caslana V, Zhu X, Li D, Jiang Q. 2001. The effectiveness of advanced rice lines with new resistance gene Xa23 to rice bacterial blight. Rice Genetics Newsletter, 18, 71–72.

Zhou Y L, Uzokwe V N E, Zhang C H, Cheng L R, Wang L, Chen K, Gao X Q, Sun Y, Chen J J, Zhu L H, Zhang Q, Ali J, Xu J L. 2011. Improvement of bacterial blight resistance of hybrid rice in China using the Xa23 gene derived from wild rice (Oryza rufipogon). Crop Protection, 30, 637–644.
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