|
|
|
|
|
| Development and identification of Verticillium wilt-resistant upland cotton accessions by pyramiding QTL related to resistance |
| GUO Xiu-hua, CAI Cai-ping, YUAN Dong-dong, ZHANG Ren-shan, XI Jing-long, GUO Wang-zhen |
| State Key Laboratory of Crop Genetics & Germplasm Enhancement, Ministry of Science and Technology/Hybrid Cotton R&D Engineering Research Center, Ministry of Education/College of Agriculture, Nanjing Agricultural University, Nanjing 210095, P.R.China |
|
|
|
摘要 Cotton Verticillium wilt is a serious soil-borne disease that leads to significant losses in fiber yield and quality worldwide. Currently, the most effective way to increase Verticillium wilt resistance is to develop new resistant cotton varieties. Lines 5026 and 60182 are two Verticillium wilt-resistant upland cotton accessions. We previously identified a total of 25 quantitative trait loci (QTLs) related to Verticillium wilt resistance from 5026 and 60182 by assembling segregating populations from hybridization with susceptible parents. In the current study, using 13 microsatellite markers flanking QTLs related to Verticillium wilt resistance, we developed 155 cotton inbred lines by pyramiding different QTLs related to Verticillium wilt resistance from a filial generation produced by crossing 5026 and 60182. By examining each allele’s effect and performing multiple comparison analysis, we detected four elite QTLs/alleles (q-5/NAU905-2, q-6/NAU2754-2, q-8/NAU3053-1 and q-13/NAU6598-1) significant for Verticillium wilt resistance, pyramiding these elite alleles increased the disease resistance of inbred lines. Furthermore, we selected 34 elite inbred lines, including five lines simultaneously performing elite fiber quality, high yield and resistance to V. dahliae, 14 lines with elite fiber quality and disease resistance, three lines with high yield and disease resistance, and 12 lines with resistance to V. dahliae. No correlation between Verticillium wilt resistance and fiber quality traits/yield and its components was detected in the 155 developed inbred lines. Our results provide candidate markers for disease resistance for use in marker-assisted breeding (MAS), as well as elite germplasms for improving important agronomic traits via modern cotton breeding.
Abstract Cotton Verticillium wilt is a serious soil-borne disease that leads to significant losses in fiber yield and quality worldwide. Currently, the most effective way to increase Verticillium wilt resistance is to develop new resistant cotton varieties. Lines 5026 and 60182 are two Verticillium wilt-resistant upland cotton accessions. We previously identified a total of 25 quantitative trait loci (QTLs) related to Verticillium wilt resistance from 5026 and 60182 by assembling segregating populations from hybridization with susceptible parents. In the current study, using 13 microsatellite markers flanking QTLs related to Verticillium wilt resistance, we developed 155 cotton inbred lines by pyramiding different QTLs related to Verticillium wilt resistance from a filial generation produced by crossing 5026 and 60182. By examining each allele’s effect and performing multiple comparison analysis, we detected four elite QTLs/alleles (q-5/NAU905-2, q-6/NAU2754-2, q-8/NAU3053-1 and q-13/NAU6598-1) significant for Verticillium wilt resistance, pyramiding these elite alleles increased the disease resistance of inbred lines. Furthermore, we selected 34 elite inbred lines, including five lines simultaneously performing elite fiber quality, high yield and resistance to V. dahliae, 14 lines with elite fiber quality and disease resistance, three lines with high yield and disease resistance, and 12 lines with resistance to V. dahliae. No correlation between Verticillium wilt resistance and fiber quality traits/yield and its components was detected in the 155 developed inbred lines. Our results provide candidate markers for disease resistance for use in marker-assisted breeding (MAS), as well as elite germplasms for improving important agronomic traits via modern cotton breeding.
|
|
Received: 26 February 2015
Accepted:
|
| Fund: This program was financially supported in part by the National Natural Science Foundation of China (31171590), the National High-Tech R&D Program of China (863 Program, 2012AA101108), the Jiangsu Agriculture Science and Technology Innovation Fund, China (cx(13)3059), and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, China (010-809001) and the Jiangsu Collaborative Innovation Center for Modern Crop Production, China (No. 10). |
|
Corresponding Authors:
GUO Wang-zhen, Tel: +86-25-84396523,E-mail: moelab@njau.edu.cn
E-mail: moelab@njau.edu.cn
|
| About author: GUO Xiu-hua, E-mail: 2011101094@njau.edu.cn; |
Cite this article:
GUO Xiu-hua, CAI Cai-ping, YUAN Dong-dong, ZHANG Ren-shan, XI Jing-long, GUO Wang-zhen.
2016.
Development and identification of Verticillium wilt-resistant upland cotton accessions by pyramiding QTL related to resistance. Journal of Integrative Agriculture, 15(3): 512-520.
|
| Afzal A J, Wood A J, Lightfoot D A. 2008. Plant receptorlikeserine threonine kinases: Roles in signaling andplant defense. Molecular Plant-Microbe Interactions, 21,507-517Bolek Y, El-Zik K M, Pepper A E, Bell A A, Magill C W, Thaxton PM, Reddy U K 2005. Mapping of Verticillium wilt resistancegenes in cotton. Plant Science, 168, 1581-1590Breseghello F, Sorrells M E. 2006. Association mapping ofkernel size and milling quality in wheat (Triticum aestivumL.) cultivars. Genetics, 172, 1165-1177Cai C, Ye W, Zhang T, Guo W. 2014. Association analysis offiber quality traits and exploration of elite alleles in uplandcotton cultivars/accessions (Gossypium hirsutum L.).Journal of Integrative Plant Biology, 56, 51-62Dixon M S, Hatzixanthis K, Jones D A, Harrison K, Jones J D.1998. The tomato Cf-5 disease resistance gene and sixhomologs show pronounced allelic variation in leucine-richrepeat copy number. The Plant Cell, 10, 1915-1925Fang H, Zhou H P, Sanogo S, Flynn R, Percy R G, Hughs SE, Ulloa M, Jones D C, Zhang J F. 2013. Quantitative traitlocus mapping for Verticillium wilt resistance in a backcrossinbred line population of cotton (Gossypium hirsutum×Gossypium barbadense) based on RGA-AFLP analysis.Euphytica, 194, 79-91Fang Z D. 1998. Research Methodology for Plant Diseases. 3rded. Chinese Agriculture Press, Beijing, China. (in Chinese)Guo W Z, Zhang T Z, Ding Y Z, Zhu Y C, Shen X L, Zhu X F.2005. Molecular marker assisted selection and pyramidingof two QTLs for fiber strength in Upland cotton. ActaGenetica Sinica, 32, 1275-1285Jiang F, Zhao J, Zhou L, Guo W Z, Zhang T Z. 2009. Molecularmapping of Verticillium wilt resistance QTL clustered onchromosomes D7 and D9 in upland cotton. Science China(Life Sciences), 52, 872-884Li C Q, Liu G S, Zhao H H, Wang L J, Zhang X F, Liu Y,Zhou W Y, Yang L L, Li P B, Wang Q L. 2013. Markerassistedselection of Verticillium wilt resistance in progenypopulations of upland cotton derived from mass selectionmasscrossing. Euphytica, 191, 469-480Li F, Fan G, Wang K, Sun F, Yuan Y, Song G, Li Q, Ma Z, LuC, Zou C, Chen W, Liang X, Shang H, Liu W, Shi C, XiaoG, Gou C, Ye W, Xu X, Zhang X, et al. 2014. Genomesequence of the cultivated cotton Gossypium arboreum.Nature Genetics, 46, 567-572Matsushima N, Miyashita H. 2012. Leucine-rich repeat(LRR) domains containing intervening motifs in plants.Biomolecules, 2, 288-311Mert M, Kurt S, Gencer O, Akiscan Y, Boyaci K, Tok F M. 2005.Inheritance of resistance to Verticillium wilt (Verticilliumdahliae) in cotton (Gossypium hirsutum L.). Plant Breeding,124, 102-104Mondragon-Palomino M, Gaut B S. 2005. Gene conversionand the evolution of three leucine-rich repeat gene familiesin Arabidopsis thaliana. Molecular Biology and Evolution,22, 2444-2456Ning Z Y, Zhao R, Chen H, Ai N J, Zhang X, Zhao J, Mei H X,Wang P, Guo W Z, Zhang T Z. 2013. Molecular tagging of amajor quantitative trait locus for broad-spectrum resistanceto Verticillium wilt in upland cotton cultivar Prema. CropScience, 53, 2304-2312Paterson A H, Brubaker C, Wendel J F. 1993. A rapid methodfor extraction of cotton (Gossypium spp.) genomic DNAsuitable for RFLP or PCR analysis. Plant Molecular BiologyReporter, 11, 122-127Paterson A H, Wendel J F, Gundlach H, Guo H, Jenkins J, JinD, Llewellyn D, Showmaker K C, Shu S, Udall J, Yoo M J,Byers R, Chen W, Doron-Faigenboim A, Duke M V, GongL, Grimwood J, Grover C, Grupp K, Hu G, et al. 2012.Repeated polyploidization of Gossypium genomes and theevolution of spinnable cotton fibres. Nature, 492, 423-427Saha S, Wu J, Jenkins J N, McCarty J C, Hayes R, StellyD M. 2011. Delineation of interspecific epistasis on fiber quality traits in Gossypium hirsutum by ADAA analysis ofintermated G. barbadense chromosome substitution lines.Theoretical and Applied Genetics, 122, 1351-1361Sal’kova E G, Guseva N N. 1965. The role of pectolytic enzymesof the Verticillium dahliae fungus in the development ofcotton wilt. Doklady Akademii Nauk SSSR, 163, 515-522Shi L Y, Wang B, Wen X. 1993. Study on strain of defoliatingtype of Verticillium wilt in cotton. Acta Gossypii Sinica, 5,89-92 (in Chinese)Song D, Li G, Song F, Zheng Z. 2008. Molecular characterizationand expression analysis of OsBISERK1, a gene encodinga leucine-rich repeat receptor-like kinase, during diseaseresistance responses in rice. Molecular Biology Reports,35, 275-283Sun Q, Jiang H, Zhu X, Wang W, He X, Shi Y, Yuan Y, DuX, Cai Y. 2013. Analysis of sea-island cotton and uplandcotton in response to Verticillium dahliae infection by RNAsequencing. BMC Genomics, 14, 852.Sun W J, Jian G L, Ma C, Shi L Y. 1997. Evaluation of cottongermplasm disease resistance using relative disease index.Plant Protection, 32, 36-37Wang H M, Lin Z X, Zhang X L, Chen W, Guo X P, Nie Y C,Li Y H. 2008. Mapping and quantitative trait loci analysisof Verticillium wilt resistance genes in cotton. Journal ofIntegrative Plant Biology, 50, 174-182Wang K, Wang Z, Li F, Ye W, Wang J, Song G, Yue Z, CongL, Shang H, Zhu S, Zou C, Li Q, Yuan Y, Lu C, Wei H, GouC, Zheng Z, Yin Y, Zhang X, Liu K, et al. 2012. The draftgenome of a diploid cotton Gossypium raimondii. NatureGenetics, 44, 1098-1103Wu T, Tian Z, Liu J, Xie C. 2009. A novel leucine-rich repeatreceptor-like kinase gene in potato, StLRPK1, is involvedin response to diverse stresses. Molecular Biology Reports,36, 2365-2374Xu L, Zhu L, Tu L, Liu L, Yuan D, Jin L, Long L, Zhang X. 2011.Lignin metabolism has a central role in the resistance ofcotton to the wilt fungus Verticillium dahliae as revealedby RNA-Seq-dependent transcriptional analysis andhistochemistry. Journal of Experimental Botany, 62,5607-5621Yang C. 2007. QTL mapping for resistance to Verticillium wilt incotton. Ph D thesis, Nanjing Agricultural University, China.(in Chinese)Yang C, Guo W Z, Li G Y, Gao F, Lin S S, Zhang T Z. 2008.QTLs mapping for Verticillium wilt resistance at seedlingand maturity stages for in G. barbadense L. Plant Science,174, 290-298Zhang J F, Fang H, Zhou H P, Sanogo S, Ma Z Y. 2014.Genetics, breeding, and marker-assisted selection forVerticillium wilt resistance in cotton. Crop Science, 54,1289-1303Zhang J, Wu Y T, Guo W Z, Zhang T Z. 2000. Fast screening ofmicrosatellite markers in cotton with PAGE/silver staining.Cotton Science, 12, 267-269 (in Chinese)Zhang X, Yuan Y, Wei Z, Guo X, Guo Y, Zhang S, Zhao J, ZhangG, Song X, Sun X. 2014. Molecular mapping and validationof a major QTL conferring resistance to a defoliating isolateof Verticillium wilt in cotton (Gossypium hirsutum L.). PLOSONE, 9, e96226.Zhang Y, Wang X F, Ding Z G, Ma Q, Zhang G R, Zhang S L,Li Z K, Wu L Q, Zhang G Y, Ma Z Y. 2013. Transcriptomeprofiling of Gossypium barbadense inoculated withVerticillium dahliae provides a resource for cottonimprovement. BMC Genomics, 14, 637.Zhao Y, Wang H, Chen W, Li Y. 2014. Genetic structure, linkagedisequilibrium and association mapping of Verticilliumwilt resistance in elite cotton (Gossypium hirsutum L.)germplasm population. PLOS ONE, 9, e86308. |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
