|
|
|
|
|
| Tissue distribution of deoxynivalenol in piglets following intravenous administration |
| DENG Xian-bai, DIN Huan-zhong, HUANG Xian-hui, MA Yong-jiang, FAN Xiao-long, YAN Hai-kuo, LU Pei-cheng, LI Wei-cheng, ZENG Zhen-ling |
| College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, P.R.China |
|
|
|
摘要 Contamination of deoxynivalenol (DON) in grains is common worldwide and pigs are particularly susceptible to this mycotoxin. The distribution of DON in porcine tissues following intravenous administration was investigated in this study. Fifteen pigs were randomly divided into three groups. Animals in groups A and B were administrated with DON at the dose of 250 and 750 μg kg–1 body weight, respectively, while group C served as blank control. Plasma, bile and 27 tissues were collected at 30 min post-administration. DON concentrations in all samples were tested using high-performance liquid chromatography- tandem mass spectrometry (HPLC-MS/MS). To observe the distribution of DON in tissues, these samples were further subjected to the immunohistochemical analyses. Totally, the bile and 13 tissues were sampled for DON-based detection, including kidney, mesenteric lymph nodes, muscle, stomach, jejunum, colon, plasma, spleen, rectum, cecum, liver, ileum, and duodenum. No significant difference was observed for the concentrations of DON in duodenum, ileum and liver samples between groups A and B; while the DON concentrations in cecum and rectum of group B were significantly higher (P-value <0.05) than those in group A. In addition, the DON concentrations in stomach, jejunum, colon, mesenteric lymph nodes, muscle, kidney, spleen, bile, and plasma of group B were remarkably higher than those of group A (P-value<0.01). Levels of DON in other 14 tissues including medulla oblongata, midbrain, diencephalon, pons, tip and tongue body, tongue, soft palate, tonsils, pharyngeal mucosa, oral buccal mucosa, thymus, thyroid, esophagus and adrenal gland were all below the limit of detection. The results of immunohistochemistry showed that 11 tissue samples (medullaoblongata, tonsil, adrenal medulla, thyroid gland, thyroid, stomach, duodenum, jejunum, kidney, spleen, and mesenteric lymph nodes) were positive and DON was mainly distributed around blood vessels in these tissues. Therefore, we believed that concentrations of DON in tissues differ when pigs are in exposure to various dosages and DON causes lesions in many pig tissues.
Abstract Contamination of deoxynivalenol (DON) in grains is common worldwide and pigs are particularly susceptible to this mycotoxin. The distribution of DON in porcine tissues following intravenous administration was investigated in this study. Fifteen pigs were randomly divided into three groups. Animals in groups A and B were administrated with DON at the dose of 250 and 750 μg kg–1 body weight, respectively, while group C served as blank control. Plasma, bile and 27 tissues were collected at 30 min post-administration. DON concentrations in all samples were tested using high-performance liquid chromatography- tandem mass spectrometry (HPLC-MS/MS). To observe the distribution of DON in tissues, these samples were further subjected to the immunohistochemical analyses. Totally, the bile and 13 tissues were sampled for DON-based detection, including kidney, mesenteric lymph nodes, muscle, stomach, jejunum, colon, plasma, spleen, rectum, cecum, liver, ileum, and duodenum. No significant difference was observed for the concentrations of DON in duodenum, ileum and liver samples between groups A and B; while the DON concentrations in cecum and rectum of group B were significantly higher (P-value <0.05) than those in group A. In addition, the DON concentrations in stomach, jejunum, colon, mesenteric lymph nodes, muscle, kidney, spleen, bile, and plasma of group B were remarkably higher than those of group A (P-value<0.01). Levels of DON in other 14 tissues including medulla oblongata, midbrain, diencephalon, pons, tip and tongue body, tongue, soft palate, tonsils, pharyngeal mucosa, oral buccal mucosa, thymus, thyroid, esophagus and adrenal gland were all below the limit of detection. The results of immunohistochemistry showed that 11 tissue samples (medullaoblongata, tonsil, adrenal medulla, thyroid gland, thyroid, stomach, duodenum, jejunum, kidney, spleen, and mesenteric lymph nodes) were positive and DON was mainly distributed around blood vessels in these tissues. Therefore, we believed that concentrations of DON in tissues differ when pigs are in exposure to various dosages and DON causes lesions in many pig tissues.
|
|
Received: 23 September 2014
Accepted:
|
| Fund: Financial support for this work was provided by the National Basic Research Program of China (2009CB118805) and the Centre for Veterinary of Drug Residues, College of Veterinary Medicine, South China Agricultural University. |
|
Corresponding Authors:
ZENG Zhen-ling, Mobile: +86-13609768425,Fax: +86-20-85280234, E-mail: zlzeng@scau.edu.cn
E-mail: zlzeng@scau.edu.cn
|
| About author: DENG Xian-bai, E-mail: xbdeng@scau.edu.cn; |
Cite this article:
DENG Xian-bai, DIN Huan-zhong, HUANG Xian-hui, MA Yong-jiang, FAN Xiao-long, YAN Hai-kuo, LU Pei-cheng, LI Wei-cheng, ZENG Zhen-ling.
2015.
Tissue distribution of deoxynivalenol in piglets following intravenous administration. Journal of Integrative Agriculture, 14(10): 2058-2064.
|
| Alexa E, Dehelean C A, Poiana M, Radulov I, Cimpean A,Bordean D, Tulcan C, Pop G. 2013. The occurrenceof mycotoxins in wheat from western Romania andhistopathological impact as effect of feed intake. ChemistryCentral Journal, 7, 99-109Ambrus Á, Szeitzn?-Szabó M, Zentai A, Sali J, Szabó I J.2011. Exposure of consumers to deoxynivalenol fromconsumption of white bread in Hungary. Food Additivesand Contaminants, 28, 209-217Amuzie C J, Harkema J R, Pestka J J. 2008. Tissue distributionand proinflammatory cytokine induction by the trichothecenedeoxynivalenol in the mouse: Comparison of nasal vs. oralexposure. Toxicilogy, 248, 39-44Anonymous. 1993. Toxins derived from Fusarium graminearum,F. culmorum and F. crookwellense: Zearalenone,deoxynivalenol, nivalenol and fusarenone X. In: IARCMonographs on the Evaluation of Carcinogenic Risks toHumans. World Health Organization, International Agencyfor Research on Cancer. Lyon, France. vol. 56. pp. 397-444Aringoli E E, Cambiagno D E, Chiericatti C A, Basilico J C,Basilico M L Z. 2012. Mycoflora study in a wheat flourmill of Argentina. Brazilian Journal of Microbiology, 43,1444-1451Berger J. 1969. IgA glomerular deposits in renal disease.Transplantation Proceedings, 1, 939-944Dänicke S, Brezina U. 2013. Kinetics and metabolism ofthe Fusarium toxin deoxynivalenol in farm animals:Consequences for diagnosis of exposure and intoxicationand carry over. Food and Chemical Toxicology, 60, 58-75Döll S, Daenicke S, Valenta H. 2008. Residues of deoxynivalenol(DON) in pig tissue after feeding mash or pellet dietscontaining low concentrations. Molecular Nutrition & FoodResearch, 52, 727-734Döll S, Dänicke S, Ueberschär K H, Valenta H, Flachowsky G.2003. Fusarium toxin residues in physiological samples ofpiglets. Mycotoxin Research, 19, 171-175Ediage E N, Di Mavungu J D, Song S, Sioen I, Saeger S D.2013. Multimycotoxin analysis in urines to assess infantexposure: A case study in Cameroon. EnvironmentInternational, 57, 50-59Forsyth D M, Yoshizawa T, Morooka N, Tuite J. 1977. Emeticand refusal activity of deoxynivalenol to swine. Applied andEnvironmental Microbiology, 34, 547-552Jelinek C F, Pohland A E, Wood G E. 1989. Worldwideoccurrence of mycotoxins in foods and feeds - An update.Journal of the Association of Official Analytical Chemists,72, 223-230Li D, Ye Y, Deng L, Ma H, Fan X, Zhang Y, Yan H, DengX, Li Y, Ma Y. 2013. Gene expression profiling analysisof deoxynivalenol-induced inhibition of mouse thymicepithelial cell proliferation. Environmental Toxicology andPharmacology, 36, 557-566Lindblad M, Gidlund A, Sulyok M, Börjesson T, Krska R, OlsenM, Fredlund E. 2013. Deoxynivalenol and other selectedFusarium toxins in Swedish wheat - Occurrence andcorrelation to specific Fusarium species. InternationalJournal of Food Microbiology, 167, 284-291Maresca M. 2013. From the gut to the brain: Journeyand pathophysiological effects of the food-associatedtrichothecene mycotoxin deoxynivalenol. Toxins, 5,784-820Pestka J J, Islam Z, Amuzie C J. 2008. Immunochemicalassessment of deoxynivalenol tissue distribution followingoral exposure in the mouse. Toxicology Letters, 178, 83-87Pestka J J, Smolinski A T. 2005. Deoxynivalenol: Toxicologyand potential effects on humans. Journal of Toxicology andEnvironmental Health (Part B Critical Reviews), 8, 39-69Poapolathep A, Poapolathep S, Sugita-Konishi Y, Wongpanit K,Machii K, Itoh Y, Kumagai S. 2010. The effect of naringeninon the fate and disposition of deoxynivalenol in piglets.Journal of Veterinary Medical Science, 72, 1289-1294Prelusky D B, Gerdes R G, Underhill K L, Rotter B A, JuiP Y, Trenholm H L. 1994. Effects of low-level dietarydeoxynivalenol on haematological and clinical parametersof the pig. Natural Toxins, 2, 97-104Rasooly L, Pestka J J. 1992. Vomitoxin-induced dysregulation of serum IgA, IgM and IgG reactive with gut bacterial andself antigens. Food and Chemical Toxicology, 30, 499-504Rodrigues I, Naehrer K. 2012. A three-year survey on theworldwide occurrence of mycotoxins in feedstuffs and feed.Toxins, 4, 663-675Rotter B A, Prelusky D B, Pestka J J. 1996. Toxicology ofdeoxynivalenol (Vomitoxin). Journal of Toxicology andEnvironmental Health, 48, 1-34Šarkanj B, Warth B, Uhlig S, Abia W A, Sulyok M, Klapec T,Banjari I. 2013. Urinary analysis reveals high deoxynivalenolexposure in pregnant women from Croatia. Food andChemical Toxicology, 62, 231-237Shephard G S, Burger H, Gambacorta L, Gong Y Y, Krska R,Rheeder J P, Solfrizzo M, Srey C, Sulyok M, Visconti A,Warth B, Westhuizen L. 2013. Multiple mycotoxin exposuredetermined by urinary biomarkers in rural subsistencefarmers in the former Transkei, South Africa. Food andChemical Toxicology, 62, 217-225Streit E, Schatzmayr G, Tassis P, Tzika E, Marin D, TaranuI, Oswald I P. 2012. Current situation of mycotoxincontamination and co-occurrence in animal feed-focus onEurope. Toxins, 4, 788-809Sugita-Konishi Y, Park B J, Kobayashi-Hattori K, Tanaka T,Chonan T, Yoshikawa K, Kumagai S. 2006. Effect of cookingprocess on the deoxynivalenol content and its subsequentcytotoxicity in wheat products. Bioscience Biotechnologyand Biochemistry, 70, 1764-1768Trenholm H L, Hamilton R M, Friend D W, Thompson B K, HartinK E. 1984. Feeding trials with vomitoxin (deoxynivalenol)-contaminated wheat: Effects on swine, poultry, anddairy cattle. Journal of the American Veterinary MedicalAssociation, 185, 527-531Young L G, McGirr L, Valli V E, Lumsden J H, Lun A. 1983.Vomitoxin in corn fed to young pigs. Journal of AnimalScience, 57, 655-664Zhang F, Wu Z Y, Wu J L, Xia Q J. 2000. Content and mutageniceffect of fusarial toxin in grains from high incidience areaswith esophageal cancer. Chinese Journal of PreventiveMedicine, 34, 53. (in Chinese) |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
