|
|
|
|
|
| Multiphasic characterization of a plant growth promoting bacterial strain, Burkholderia sp. 7016 and its effect on tomato growth in the field |
| GAO Miao, ZHOU Jian-jiao, WANG En-tao, CHEN Qian, XU Jing, SUN Jian-guang |
1、Key Laboratory of Microbial Resources, Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning, Chinese cademy of Agricultural Sciences, Beijing 100081, P.R.China
2、Departmento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Polytécnico National, Mexico D F 11340,Mexico |
|
|
|
摘要 Aiming at searching for plant growth promoting rhizobacteria (PGPR), a bacterium strain coded as 7016 was isolated from soybean rhizosphere and was characterized in the present study. It was identified as Burkholderia sp. based on 16S rDNA sequence analysis, as well as phenotypic and biochemical characterizations. This bacterium presented nitrogenase activity, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity and phosphate solubilizing ability; inhibited the growth of Sclerotinia sclerotiorum, Gibberella zeae and Verticillium dahliae; and produced small quantities of indole acetic acid (IAA). In green house experiments, significant increases in shoot height and weight, root length and weight, and stem diameter were observed on tomato plants in 30 d after inoculation with strain 7016. Result of 16S rDNA PCR-DGGE showed that 7016 survived in the rhizosphere of tomato seedlings. In the field experiments, Burkholderia sp. 7016 enhanced the tomato yield and significantly promoted activities of soil urease, phosphatase, sucrase, and catalase. All these results demonstrated Burkholderia sp. 7016 as a valuable PGPR and a candidate of biofertilizer.
Abstract Aiming at searching for plant growth promoting rhizobacteria (PGPR), a bacterium strain coded as 7016 was isolated from soybean rhizosphere and was characterized in the present study. It was identified as Burkholderia sp. based on 16S rDNA sequence analysis, as well as phenotypic and biochemical characterizations. This bacterium presented nitrogenase activity, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity and phosphate solubilizing ability; inhibited the growth of Sclerotinia sclerotiorum, Gibberella zeae and Verticillium dahliae; and produced small quantities of indole acetic acid (IAA). In green house experiments, significant increases in shoot height and weight, root length and weight, and stem diameter were observed on tomato plants in 30 d after inoculation with strain 7016. Result of 16S rDNA PCR-DGGE showed that 7016 survived in the rhizosphere of tomato seedlings. In the field experiments, Burkholderia sp. 7016 enhanced the tomato yield and significantly promoted activities of soil urease, phosphatase, sucrase, and catalase. All these results demonstrated Burkholderia sp. 7016 as a valuable PGPR and a candidate of biofertilizer.
|
|
Received: 26 September 2014
Accepted:
|
| Fund: This research was supported by the National Natural Science Foundation of China (31100364) and the National Nonprofit Institute Research Grant of Chinese Academy of Agricultural Sciences (CAAS, IARRP-2014-20). |
|
Corresponding Authors:
SUN jian-guang, Tel: +86-10-82108701,E-mail: sunjianguang@caas.cn
E-mail: sunjianguang@caas.cn
|
| About author: GAO Miao, Tel: +86-10-82108701, E-mail: gaomiao@caas.cn; |
Cite this article:
GAO Miao, ZHOU Jian-jiao, WANG En-tao, CHEN Qian, XU Jing, SUN Jian-guang.
2015.
Multiphasic characterization of a plant growth promoting bacterial strain, Burkholderia sp. 7016 and its effect on tomato growth in the field. Journal of Integrative Agriculture, 14(9): 1855-1863.
|
| Ahmad M, Zahir Z A, Khalid M, Nazli F, Arshad M. 2013.Efficacy of Rhizobium and Pseudomonas strains to improvephysiology, ionic balance and quality of mung bean undersalt-affected conditions on farmer’s fields. Plant Physiologyand Biochemistry, 63, 170-176Ahmed A, Hasnain S. 2010. Auxin-producing Bacillus sp.:Auxin quantification and effect on the growth of Solanumtuberosum. Pure and Applied Chemistry, 82, 313-319Almaghrabi O A, Massoud S I, Abdelmoneim T S. 2013.Influence of inoculation with plant growth promotingrhizobacteria (PGPR) on tomato plant growth and nematodereproduction under greenhouse conditions. Saudi Journalof Biological Sciences, 20, 57-61Amprayn K, Rose M T, Kecskés M, Pereg L, Nguyen H T,Kennedy I R. 2012. Plant growth promoting characteristicsof soil yeast (Candida tropicalis HY) and its effectiveness forpromoting rice growth. Applied Soil Ecology, 61, 295-299Atlas R M. 1993. Handbook of Microbiological Media. CRCPress, Boca Raton, FL.Barnawal D, Bharti N, Maji D, Chanotiya C S, Kalra A. 2012.1-Aminocyclopropane-1-carboxylic acid (ACC) deaminasecontainingrhizobacteria protect Ocimum sanctum plantsduring waterlogging stress via reduced ethylene generation.Plant Physiology and Biochemistry, 58, 227-235Beneduzi A, Peres D, Vargas L K, Bodanese-Zanettini M H,Passaglia L M P. 2008. Evaluation of genetic diversity andplant growth promoting activities of nitrogen-fixing bacilliisolated from rice fields in South Brazil. Applied Soil Ecology,39, 311-320Chen Q, Hu H, Gao M, Xu J, Zhou Y, Sun J. 2011. Screeningand identification of a nitrogen fixing bacteria with1-aminocyclopropane-1-carboxylate deaminase activity.Plant Nutrition and Fertilizer Science, 17, 1515-1521 (inChinese)Cheng Z, Woody O Z, McConkey B J, Glick B R. 2012.Combined effects of the plant growth-promoting bacteriumPseudomonas putida UW4 and salinity stress on theBrassica napus proteome. Applied Soil Ecology, 61,255-263Crozier J A, Boothroyd C W. 1959. Tomato: a new suscept ofGibberella zeae (Schw.) Petch. Plant Diseases and Pests,43, 446-447Eivazi F, Bayan M. 2002. Effect of long-term fertilization andcropping systems on selected soil enzyme activities. Journalof Plant Nutrition, 92, 686-687Fankem H, Nwaga D, Deubel A, Dieng L, Merbach W, Etoa F X.2006. Occurrence and functioning of phosphate solubilizingmicroorganisms from oil palm tree (Elaeis guineensis)rhizosphere in Cameroon. African Journal of Biotechnology,5, 2450-2460Ferrari V C, Hollibaugh J T. 1999. Distribution of microbialassemblages in the Central Arctic Ocean Basin studied byPCR/DGGE: Analysis of a large data set. Hydrobiologia,401, 55-68Figueiredo M V, Seldin L, de Araujo F, Mariano R L R. 2010.Plant growth promoting rhizobacteria: fundamentals andapplications. In: Maheshwari D K ed., Plant Growth AndHealth Promoting Bacteria, Microbiology Monographs.Springer Verlag, Berlin.Garau G, Yates R J, Deiana P, Howieson J G. 2009. Novelstrains of nodulating Burkholderia have a role in nitrogenfixation with papilionoid herbaceous legumes adaptedto acid, infertile soils. Soil Biology and Biochemistry,41,125-134Glick B R, Karaturovic D M, Newell P C. 1995. A novel procedurefor rapid isolation of plant growth promoting Pseudomonads.Canadian Journal of Microbiology, 41, 533-536Guan S Y, Shen G Q. 1986. Enzyme activities in main soil inChina. Acta Pedologica Sinica, 21, 368-381 (in Chinese)Harrigan W, McCance M. 1976. Laboratory Methods in Foodsand Dairy Microbiology. Academic Press, London. pp66-81Ji J J, Zhang X F, Wang W Q, Zhang J L. 2012. Researchprogress on control of tomato gray mold. ChineseAgricultural Science Bulletin, 28, 109-113Jiang R B, Ning G Z. 2001. List of Agricultural Culturaes ofChina. China Agricultural Scientech Press, China. (inChinese)Johnson J L, Temple K L. 1964. Some variables affectingthe measurement of catalase activity in soil. Soil ScienceSociety of America Proceedings, 28, 207-209Kang S M, Hamayun M, Joo G J, Khan A L, Kim Y H, Kim S K,Jeong H J, Lee I J. 2010. Effect of Burkholderia sp. KCTC11096BP on some physiochemical attributes of cucumber.European Journal of Soil Biology, 46, 264-268Lane D J. 1991. 16S/23S rRNA sequencing. In: StackebrandtE, Goodfellow M, eds., Nucleic Acid Techniques in BacterialSystematics. New York. Wiley. pp. 115-175 Muyzer G, Waal E C, Uitterlinden A G. 1993. Profiling ofcomplex microbial populations by denaturing gradient gelelectrophoresis analysis of polymerase chain reactionamplifiedgenes coding for 16S rRNA. Applied EnvironmentalMicrobiology, 59, 695-700Nadeem S M, Shaharoona B, Arshad M, Crowley D E. 2012.Population density and functional diversity of plant growthpromoting rhizobacteria associated with avocado trees insaline soils. Applied Soil Ecology, 62, 147-154Pariona-Llanos R, Ferrara F I S, Gonzales H H S, Barbosa HR. 2010. Influence of organic fertilization on the number ofculturable diazotrophic endophytic bacteria isolated fromsugarcane. European Journal of Soil Biology, 46, 387-393Peix A, Mateos P F, Odriguez-Barrueco C R, Martinez-MolinaE, Velazquez E. 2001. Growth promotion of commonbean (Phaseolus vulgaris L.) by a strain of Burkholderiacepacia under growth chamber conditions. Soil Biology andBiochemistry, 33, 1927-1935Penrose D M, Glick B R. 2003. Methods for isolating andcharacterizing ACC deaminase-containing plant growthpromotingrhizobacteria. Physiologia Plantarum, 118,10-15Rashid S, Charles T C, Glick B R. 2012. Isolation andcharacterization of new plant growth-promoting bacterialendophytes. Applied Soil Ecology, 61, 217-224Raymond J, Siefert J L, Staples C R, Blankenship R E. 2004.The natural history of nitrogen fixation. Molecular Biologyand Evolution, 21, 541-554Recep K, Fikrettin S, Erkol D, Cafer E. 2009. Biological controlof the potato dry rot caused by Fusarium species usingPGPR strains. Biological Control, 50, 194-198Reyes I, Bernier L, Simard R R, Tanguay P, Antoun H. 1999.Characteristics of phosphate solubilization by an isolate of atropical Penicillium rugulosum and two UV-induced mutants.FEMS Microbiology Ecology, 28, 291-295.Saitou N, Nei M. 1987. The neighbor-joining method: A newmethod for reconstructing phylogenetic trees. MolecularBiology and Evolution, 4, 406-425Shanmugam V, Kanoujia N. 2011. Biological management ofvascular wilt of tomato caused by Fusarium oxysporumf. sp. lycospersici by plant growth-promoting rhizobacterialmixture. Biological Control, 57, 85-93Shanmugam V, Kanoujia N, Singh M, Singh S, Prasad R.2011. Biocontrol of vascular wilt and corm rot of gladioluscaused by Fusarium oxysporum f. sp. gladioli using plantgrowth promoting rhizobacterial mixture. Crop Protection,30, 807-813Sharp R G, Chen L, Davies W J. 2011. Inoculation of growingmedia with the rhizobacterium Variovorax paradoxus 5C-2reduces unwanted stress responses in hardy ornamentalspecies. Scientia Horticculturae, 129, 804-811Shoebitz M, Ribaudo C M, Pardo M A, Cantore M L, CiampiL, Cura J A. 2009. Plant growth promoting properties of astrain of Enterobacter ludwigii isolated from Lolium perennerhizosphere. Soil Biology and Biochemistry, 41, 1768-1774Srinivasan S, Kim J, Kang S-R, Jheong W H, Lee S S. 2013.Burkholderia humi sp. nov., isolated from peat soil.Current Microbiology, 66, 300-305Smibert R M, Krieg N R. 1994. Phenotypic characterization. In:Gerhardt P, Murray R G E, Wood W A, Krieg M N R, eds.,Manual of Methods for General and Microbiology. AmericanSociety for Microbiology, Washington, D. C. pp. 607-654Stewart K J, Coxson D, Siciliano S D. 2011. Small-scalespatial patterns in N2-fixation and nutrient availability inan arctic hummocke-hollow ecosystem. Soil Biology andBiochemistry, 43, 133-140Sun Y M, Zhang N N, Wang E T, Yuan H L, Yang J S, Chen WX. 2009. Influence of intercropping and intercropping plusrhizobial inoculation on microbial activity and communitycomposition in rhizosphere of alfalfa (Medicago sativaL.) and Siberian wild rye (Elymus sibiricus L.). FEMSMicrobiology Ecology, 70, 218-226Tabatabai M A. 1994. Soil enzymes. In: Weaver R W, Angle JS, Bottomley P S, eds., Methods of Soil Analysis, Part 2.Microbiological and Biochemical Properties. Soil ScienceSociety of America, Madison, USA. pp. 775-833Thompson J D, Gibson T J, Plewniak F, Jeanmougin F, HigginsD G. 1997. The Clustal_X windows interface: Flexiblestrategies for multiple sequence alignment aided by qualityanalysis tools. Nucleic Acids Research, 25, 4876-4882Ventosa A, Quesada E, Rodriguez-Valera F, Ruiz-BerraqueroF, Ramos-Cormenzana A. 1982. Numerical taxonomyof moderately halophilic Gram-negative rods. Journal ofGeneral and Applied Microbiology, 128, 1959-1968Wang H, Wen K, Zhao X, Wang X, Li A, Hong H. 2009. Theinhibitory activity of endophytic Bacillus sp. strain CHM1against plant pathogenic fungi and its plant growthpromotingeffect. Crop Protection, 28, 634-639Wang X, Haruta S, Wang P, Ishii M, Igarashi Y, Cui Z. 2006.Diversity of a stable enrichment culture which is useful forsilage inoculant and its succession in alfalfa silage. FEMSMicrobiology Ecology, 57, 106-115Woo P C Y, Lau S K P, Teng J L L, Tse H, Yuen K Y. 2008.Then and now: Use of 16S rDNA gene sequencing forbacterial identification and discovery of novel bacteria inclinical microbiology laboratories. Clinical Microbiology andInfection, 14, 908-934Xie G H, Cai M Y, Tao G C, Steinberger Y. 2003. Cultivableheterotrophic N2-fixing bacterial diversity in rice fields in theYangtze River. Plain Biology Fertility of Soils, 37, 29-38Yang L, Bai Y, Wang L, Wang H, Lu Y. 2011. Study on themethod of determining available phosphorus (Olsen-P)with high testing efficiency. Soil and Fertilizer Sciences inChina, 3, 87-91 (in Chinese)Yoon J, Kim H, Kim S, Kim H, Kim W, Lee S, Goodfellow M,Park Y. 1996. Identification of Saccharomonospora strainsby the use of genomic DNA fragments and rRNA geneprobes. International Journal of Systematic and EvolutionaryMicrobiology, 46, 502-505 |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
