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| Myeloid zinc finger 1 (MZF1) is the most important transcriptional factor for porcine follistatin promoter |
| SUN Ya-meng, WANG Liang, YANG Xiu-qin, ZHANG Dong-jie, LIU Di |
1、College of Animal Sciences and Technology, Northeast Agricultural University, Harbin 150030, P.R.China
2、Heilongjiang Academy of Agricultural Sciences, Harbin 150086, P.R.China |
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摘要 Follistatin (FS) is a secreted protein, which was originally isolated from porcine follicular fluid. Expression of follistatin is tightly regulated during porcine growth and development. To study the essential transcriptional regions of the porcine FS promoter, ten primer pairs were designed to amplify segments with different lengths of the FS promoter from –1 800 to +16 bp. The products were then inserted into the pGL3-basic vector to analyze the relative luciferase activity. The results showed that the most remarkable changes of promoter activity were observed between constructs (–302/+16 bp)-FS and (–180/+16 bp)-FS (P<0.01). Further research showed that the reconstructed reporter plasmid lacking myeloid zinc finger 1 (MZF1) binding sequence had significantly decreased luciferase activity (P<0.05). Furthermore, the FS protein expression was significantly increased in PK15 cells while the MZF1 was overexpressed, suggesting that the short sequence “TCCCCACC” (the recognition site of transcription factor MZF1) was the most important for FS transcription activation in the porcine.
Abstract Follistatin (FS) is a secreted protein, which was originally isolated from porcine follicular fluid. Expression of follistatin is tightly regulated during porcine growth and development. To study the essential transcriptional regions of the porcine FS promoter, ten primer pairs were designed to amplify segments with different lengths of the FS promoter from –1 800 to +16 bp. The products were then inserted into the pGL3-basic vector to analyze the relative luciferase activity. The results showed that the most remarkable changes of promoter activity were observed between constructs (–302/+16 bp)-FS and (–180/+16 bp)-FS (P<0.01). Further research showed that the reconstructed reporter plasmid lacking myeloid zinc finger 1 (MZF1) binding sequence had significantly decreased luciferase activity (P<0.05). Furthermore, the FS protein expression was significantly increased in PK15 cells while the MZF1 was overexpressed, suggesting that the short sequence “TCCCCACC” (the recognition site of transcription factor MZF1) was the most important for FS transcription activation in the porcine.
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Received: 28 May 2014
Accepted:
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| Fund: This research was supported by the National Natural Science Foundation of China (31301955) and the China Agriculture Research System (CARS-36). |
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Corresponding Authors:
LIU Di, Mobile: +86-13803644211, E-mail: liudi1963@163.com
E-mail: liudi1963@163.com
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| About author: SUN Ya-meng, E-mail: symbuster_1986@163.com; |
Cite this article:
SUN Ya-meng, WANG Liang, YANG Xiu-qin, ZHANG Dong-jie, LIU Di.
2015.
Myeloid zinc finger 1 (MZF1) is the most important transcriptional factor for porcine follistatin promoter. Journal of Integrative Agriculture, 14(7): 1383-1389.
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