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| Molecular Characteristics of New Wheat Starch and Its Digestion Behaviours |
| ZHOU Zhong-kai, HUA Ze-tian, YANG Yan, ZHENG Pai-yun, ZHANG Yan , CHEN Xiao-shan |
1、Key Laboratory of Food Nutrition and Safety, Ministry of Education/Tianjin University of Science and Technology, Tianjin 300457, P.R.China
2、China National Japonica Rice R&D Center, Tianjin 300457, P.R.China |
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摘要 In order to understand the effect of starch molecular characteristics on the gel structure, which subsequently influence the gel digestion behaviours, three wheat starches, control (conventional wheat starch), two new wheat cultivars with different genetic backgrounds (by knocking out SBE IIb and SBE IIa, respectively) were used in this study. In comparison with control, slight differences in the morphology of the starch granules of new wheat 1 were observed, whereas the starch granules of new wheat 2 had irregular shapes both for A-type granules and B-type granules. Starch molecular weight size was determined by SE-HPLC, and the results indicate that there was a subtle increase in the amylose content in the starch of new wheat 1 compared to that of control. The starch of new wheat 2 had the highest amylose content, and the molecular weight (MW) of its amylopectin was the lowest among the three starches. Fourier transform infrared spectroscopy (FTIR) was employed to investigate starch gel structure and the results suggest that the molecules of starch gel from new wheat 2 are more likely to re-associate to form an organized conformation. The digestion behaviours of the three starch gels were measured using a mixture of pancreatin α-amylase and amyloglucosidase. The results indicated that the starch gels of control and new wheat 1 had very high digestibility of 91.7 and 91.9%, respectively, whereas the digestibility of wheat 2 starch gel was only 36.2%. In comparison with the digestion curve patterns of control and new wheat 1 starch gels, the new wheat 2 exhibited a much lower initial velocity. These results indicated that the molecules in the starch of new wheat 2 are more readily to re-associate to form an organized structure during gel formation because of its unique molecular characteristics.
Abstract In order to understand the effect of starch molecular characteristics on the gel structure, which subsequently influence the gel digestion behaviours, three wheat starches, control (conventional wheat starch), two new wheat cultivars with different genetic backgrounds (by knocking out SBE IIb and SBE IIa, respectively) were used in this study. In comparison with control, slight differences in the morphology of the starch granules of new wheat 1 were observed, whereas the starch granules of new wheat 2 had irregular shapes both for A-type granules and B-type granules. Starch molecular weight size was determined by SE-HPLC, and the results indicate that there was a subtle increase in the amylose content in the starch of new wheat 1 compared to that of control. The starch of new wheat 2 had the highest amylose content, and the molecular weight (MW) of its amylopectin was the lowest among the three starches. Fourier transform infrared spectroscopy (FTIR) was employed to investigate starch gel structure and the results suggest that the molecules of starch gel from new wheat 2 are more likely to re-associate to form an organized conformation. The digestion behaviours of the three starch gels were measured using a mixture of pancreatin α-amylase and amyloglucosidase. The results indicated that the starch gels of control and new wheat 1 had very high digestibility of 91.7 and 91.9%, respectively, whereas the digestibility of wheat 2 starch gel was only 36.2%. In comparison with the digestion curve patterns of control and new wheat 1 starch gels, the new wheat 2 exhibited a much lower initial velocity. These results indicated that the molecules in the starch of new wheat 2 are more readily to re-associate to form an organized structure during gel formation because of its unique molecular characteristics.
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Received: 19 November 2012
Accepted:
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| Fund: This work was supported by the China-Euro Collaboration Funds from the Minister of Science and Technology of China (SQ2013ZOA100001). |
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Corresponding Authors:
ZHOU Zhong-kai, Tel: +86-22-60601442, E-mail: zhongkai_zhou@hotmail.com
E-mail: zhongkai_zhou@hotmail.com
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Cite this article:
ZHOU Zhong-kai, HUA Ze-tian, YANG Yan, ZHENG Pai-yun, ZHANG Yan , CHEN Xiao-shan.
2014.
Molecular Characteristics of New Wheat Starch and Its Digestion Behaviours. Journal of Integrative Agriculture, 13(5): 1146-1153.
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| Barcelo A, Claustre J, Moro F, Chayvialle J A, Cuber J C, Plaisancie P. 2000. Mucin secretion is modulated by luminal factors in the isolated vascularly perfused rat colon. Gut, 46, 218-224 Bernazzani P, Chapados C, Delmas G. 2001. Phase change in amylose-water mixtures as seen by Fourier transform infrared. Biopolymers, 58, 305-318 Bhattacharyya M K, Smith A M, Noel Ellis T H, Hedley C, Martin C. 1990. The wrinkled-seed character of pea described by Mendel is caused by a transposon-like insertion in a gene encoding starch-branching enzyme. Cell, 60, 115-122 Bird A R, Brown I L, Topping D L. 2000. Starches, resistant starches, the gut microflora and human health. Current Issues in Intestinal Microbiology, 1, 25-37 Björck I M, Granfeldt Y, Liljeberg H, Tovar J, Asp N G. 1994. Food properties affecting the digestion and absorption of carbohydrates. The American Journal of Clinical Nutrition, 59, 699S-705S Englyst H N, Hudson G J. 1996. The classification and measurement of dietary carbohydrates. Food Chemistry, 57, 15-21 Englyst H N, Kingman S M, Cummings J H. 1992. Classification and measurement of nutritionally important starch fractions. European Journal of Clinical Nutrition, 46, S33-S50. Englyst H N, Trowell H, Southgate D A, Cummings J H. 1987. Dietary fiber and resistant starch. The American Journal of Clinical Nutrition, 46, 873-874 Fechner P M, Wartewig S, Kleinebudde P, Neubert R H H. 2005. Studies of the retrogradation process for various starch gels using Raman spectroscopy. Carbohydrate Research, 340, 2563-2568 Gaudier E, Jarry A, Blottiere H M, Coppet P, Buisine M P, Aubert J P, Laboisse C, Cherbut C, Hoebler C. 2004. Butyrate specifically modulates MUC gene expression in intestinal epithelial goblet cells deprived of glucose. American Journal of Physiology Gastrointestinal and Liver Physiology, 287, G1168-G1174. Godet M C, Tran V, Delage M M, Buleon A. 1993. Molecular modelling of the specific interactions involved in the amylose complexation by fatty acids. International Journal of Biological Macromolecules, 15, 11-16 Hoover R, Sosulski F W 1991. Composition, structure, functionality and chemical modification of legume starches-a review. Canadian Journal of Physiology and Pharmacology, 69, 79-92 Ludwig D S. 2002. The glycemic index: physiological mechanisms relating to obesity, diabetes, and cardiovascular disease. Journal of the American Medical Association, 287, 2414-2423 Ludwig D S. 2003. Glycemic load comes of age. The Journal of Nutrition, 133, 2695-2696 Mizuno K, Kawasaki T, Shimada H, Satoh H, Kobayashi E, Okumura S, Arai Y, Baba T. 1993. Alteration of the structural properties of starch components by the lack of an isoform of starch branching enzyme in rice seeds. The Journal of Biological Chemistry, 268, 19084-19091 Putaux J L, Buléon A, Chanzy H. 2000. Network formation in dilute amylose and amylopectin studied by TEM. Macromolecules, 33, 6416-6422 Skrabanja V, Liljeberg H G M, Hedley C L, Kreft I, Björck I M E. 1999. Influence of genotype and processing on the in vitro rate of starch hydrolysis and resistant starch hydrolysis in peas (Pisum sativum L.). Journal of Agricultural and Food Chemistry, 47, 2033-2039 Slaughter S L, Ellis P R, Butterworth P J. 2001. An investigation of the action of porcine pancreatic α-amylase on native and gelatinised starches. Biochimica et Biophysica Acta, 1525, 29-36 Stinard P S, Robertson D S, Schnable P S. 1993. Genetic isolation, cloning, and analysis of a mutator-induced, dominant antimorph of the maize amylose extender1 Locus. The Plant Cell, 5, 1555-1566 Topping D L, Clifton P M. 2001. Short-chain fatty acids and human colonic function: Roles of resistant starch and nonstarch polysaccharides. Physiological Reviews, 81, 1031-1064 Tovar J, Björck I M, Asp N G. 1992. Incomplete digestion of legume starches in rats: A study of precooked flours containing retrograded and physically inaccessible starch fractions. The Journal of Nutrition, 122, 1500-1507 Tovar J, Melito C C, Herrera E, Rascón A, Pérez E. 2002. Resistant starch formation does not parallel syneresis tendency in different starch gels. Food Chemistry, 76, 455-459 Waigh T A, Kato K L, Donald A M, Gidley M J, Clarke C J, Riekel C. 2000. Side-chain liquid crystalline model for starch. Starch/Stärke, 52, 450-460. |
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