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Journal of Integrative Agriculture  2014, Vol. 13 Issue (5): 1137-1145    DOI: 10.1016/S2095-3119(13)60374-3
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Solvent Effects on the Ultrastructure and Chemical Composition of Cuticular Wax and Its Potential Bioactive Role Against Alternaria alternata in Pingguoli Pear
 CHEN Song-jiang, LI Yong-cai, BI Yang, YIN Yan, GE Yong-hong , WANG Yi
College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, P.R.China
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摘要  Effects of different polarity solvents on the ultrastructure and chemical composition of cuticular wax in Pingguoli pear as well as their bioactive role against Alternaria alternate were studied and the results showed that the highest wax content was extracted with chloroform, and its wax content was up to 322.2 μg cm-2. Long-chain fatty acids predominated in menthol extracts and n-alkanes were predominant in wax extracted with ether, chloroform and n-hexane. Pingguoli pear fruit surface was covered by a smooth and amorphous wax layer with small, scattered crystal. The morphology of recrystallized wax in vitro after removal with different solvents was not similar to that of the intact fruit surface. Removal of cuticular wax with various solvents significantly enhanced A. alternata infection, except for wax removed by methanol. The solvent extracts of methanol and chloroform stimulated the spore germination and mycelium growth of A. alternata, but the ether and n-hexane extracts showed antifungal activity.

Abstract  Effects of different polarity solvents on the ultrastructure and chemical composition of cuticular wax in Pingguoli pear as well as their bioactive role against Alternaria alternate were studied and the results showed that the highest wax content was extracted with chloroform, and its wax content was up to 322.2 μg cm-2. Long-chain fatty acids predominated in menthol extracts and n-alkanes were predominant in wax extracted with ether, chloroform and n-hexane. Pingguoli pear fruit surface was covered by a smooth and amorphous wax layer with small, scattered crystal. The morphology of recrystallized wax in vitro after removal with different solvents was not similar to that of the intact fruit surface. Removal of cuticular wax with various solvents significantly enhanced A. alternata infection, except for wax removed by methanol. The solvent extracts of methanol and chloroform stimulated the spore germination and mycelium growth of A. alternata, but the ether and n-hexane extracts showed antifungal activity.
Keywords:  fruit cuticular membrane       Alternaria alternata       antifungal or stimulus activity  
Received: 20 November 2012   Accepted:
Fund: 

This research was financially supported by the National Natural Sciences Foundation of China (30960243 and 31260494), and the Fuxi Distinguished Young Talent Cultivation Project of Gansu Agricultural University, China.

Corresponding Authors:  LI Yong-cai, Tel: +86-931-7632256, Fax: +86-931-7631201, E-mail: liyongcai@gsau.edu.cn     E-mail:  liyongcai@gsau.edu.cn
About author:  CHENG Song-jiang, E-mail: 409770766@qq.com

Cite this article: 

CHEN Song-jiang, LI Yong-cai, BI Yang, YIN Yan, GE Yong-hong , WANG Yi. 2014. Solvent Effects on the Ultrastructure and Chemical Composition of Cuticular Wax and Its Potential Bioactive Role Against Alternaria alternata in Pingguoli Pear. Journal of Integrative Agriculture, 13(5): 1137-1145.

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