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| Insecticidal Constructure and Bioactivities of Compounds from Ficus sarmentosa var. henryi |
| WANG Xue-gui, WEI Xiao-yi, HUANG Xing-yan, SHEN Li-tao, TIAN Yong-qing , XU Han-hong |
1. Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education/South China Agricultural University
2. Biorational Pesticide Research Laboratory, Sichuan Agricultural University
3. South China Institute of Botany, Chinese Academy of Sciences |
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摘要 Insecticidal activities of the petroleum ether-, chloroform-, ethyl acetate-, and water-soluble fractions of the methanolicextract of Ficus sarmentosa var. henryi were assayed against Musca domestica adults. The chloroform- and ethyl acetatesolublefractions were the most active with 92.6 and 88.9% mortalities (24 h after treatment) respectively. Therefore, thetwo fractions were combined and four compounds, isolated from the fractions by activity-guided fractionation, wereelucidated as 7-hydroxycoumarin, apigenin, eriodictyol, and quercetin by spectroscopic method and displayed excellentinsecticidal activities against adults of M. domestica and 4th instar larva of Aedes albopictus. Among those, 7-hydroxycoumarin showed the strongest insecticidal activities with lethal concentrations (LC50) values of 72.13 μg g-1sugar and 4.87 μg mL-1 (48 h after treatment) against the test insects respectively. The cytoxicities of these compounds onBTI-Tn-5B1-4 cell were also investigated for the insecticidal mechanism and found that quercetin represented superiorinhibitory activity with MTT assay and reactive oxygen species (ROS) against BTI-Tn-5B1-4 cell, but slightly weaker thanthat of the positive control (azadirachtin) and significantly greater than the negative control (DMSO only). Meanwhile,eriodictyol demonstrated the strongest effect on the mitochondrial membrane potentials (MMP). In conclusion, based ontheir comparative toxicities to commercial insecticides and their cytotoxic effects, some of the compounds from theF. sarmentosa have potential as botanical insecticides.
Abstract Insecticidal activities of the petroleum ether-, chloroform-, ethyl acetate-, and water-soluble fractions of the methanolicextract of Ficus sarmentosa var. henryi were assayed against Musca domestica adults. The chloroform- and ethyl acetatesolublefractions were the most active with 92.6 and 88.9% mortalities (24 h after treatment) respectively. Therefore, thetwo fractions were combined and four compounds, isolated from the fractions by activity-guided fractionation, wereelucidated as 7-hydroxycoumarin, apigenin, eriodictyol, and quercetin by spectroscopic method and displayed excellentinsecticidal activities against adults of M. domestica and 4th instar larva of Aedes albopictus. Among those, 7-hydroxycoumarin showed the strongest insecticidal activities with lethal concentrations (LC50) values of 72.13 μg g-1sugar and 4.87 μg mL-1 (48 h after treatment) against the test insects respectively. The cytoxicities of these compounds onBTI-Tn-5B1-4 cell were also investigated for the insecticidal mechanism and found that quercetin represented superiorinhibitory activity with MTT assay and reactive oxygen species (ROS) against BTI-Tn-5B1-4 cell, but slightly weaker thanthat of the positive control (azadirachtin) and significantly greater than the negative control (DMSO only). Meanwhile,eriodictyol demonstrated the strongest effect on the mitochondrial membrane potentials (MMP). In conclusion, based ontheir comparative toxicities to commercial insecticides and their cytotoxic effects, some of the compounds from theF. sarmentosa have potential as botanical insecticides.
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Received: 08 October 2010
Accepted:
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| Fund: National Natural Science Foundation of China (30571235). |
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Corresponding Authors:
Correspondence XU Han-hong, Professor, Tel: +86-20-85285127, E-mail: hhxu@scau.edu.cn
E-mail: hhxu@scau.edu.cn
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| About author: WANG Xue-gui, Ph D, E-mail: wanderwxg@126.com |
Cite this article:
WANG Xue-gui, WEI Xiao-yi, HUANG Xing-yan, SHEN Li-tao, TIAN Yong-qing , XU Han-hong.
2011.
Insecticidal Constructure and Bioactivities of Compounds from Ficus sarmentosa var. henryi. Journal of Integrative Agriculture, 10(9): 1402-1409.
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