potato, recurrent selection, late blight, horizontal resistance, genetic diversity," /> potato, recurrent selection, late blight, horizontal resistance, genetic diversity,"/> potato, recurrent selection, late blight, horizontal resistance, genetic diversity,"/> Population Improvement of Resistance to Late Blight in Tetraploid Potato: A Case Study in Combination with AFLP Marker Assisted Background Selection
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Journal of Integrative Agriculture  2011, Vol. 10 Issue (8): 1177-1187    DOI: 10.1016/S1671-2927(11)60108-9
GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS Advanced Online Publication | Current Issue | Archive | Adv Search |
Key Laboratory of Horticultural Plant Biology, Ministry of Education/National Center for Vegetable Improvement (Central China)/Potato Engineering and Technology Research Center of Hubei Province/Huazhong Agricultural University
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摘要  Cultivated potato with high level of horizontal resistance against late blight is one of the most important goals of potatobreeding. The recurrent selection has been adopted to increase the level of potato horizontal resistance and a B3C1population without R1-R11 dominant genes has been released by the International Potato Center at the short-day conditionof Peru. The present research was carried out to further improve the resistance and the agronomic traits of B3C1population under long-day condition of Hubei, China, with maximized retention of its genetic diversity. Twenty-sevenindividual clones of B3C1 were used to generate population B3C2 by in-population crossing with the bulk pollens aimingto elevate the frequency of late blight resistance genotypes and to improve the adaptation to local long-day conditions.The late blight resistance and the main agronomic traits including the maturity, the plant characters and the tuber traitswere evaluated for the foreground selection in three years, by which 130 pedigrees were maintained as the basic populationof B3C2 for further selection. A total of 312 polymorphic loci detected by 9 AFLP marker combinations were used tomonitor the genetic diversity of the populations for the background selection. The B3C2 population of 51 clones wasfinally selected, of which the frequency of resistant genotypes increased by 23.8% points and the genetic diversity wasmaintained by about 96% as referred to B3C1. Our results strongly suggested that combination of the foregroundselection for target traits and the background selection for the genetic diversity is an efficient strategy in the recurrentselection of tetraploid potato to improve quantitative traits.

Abstract  Cultivated potato with high level of horizontal resistance against late blight is one of the most important goals of potatobreeding. The recurrent selection has been adopted to increase the level of potato horizontal resistance and a B3C1population without R1-R11 dominant genes has been released by the International Potato Center at the short-day conditionof Peru. The present research was carried out to further improve the resistance and the agronomic traits of B3C1population under long-day condition of Hubei, China, with maximized retention of its genetic diversity. Twenty-sevenindividual clones of B3C1 were used to generate population B3C2 by in-population crossing with the bulk pollens aimingto elevate the frequency of late blight resistance genotypes and to improve the adaptation to local long-day conditions.The late blight resistance and the main agronomic traits including the maturity, the plant characters and the tuber traitswere evaluated for the foreground selection in three years, by which 130 pedigrees were maintained as the basic populationof B3C2 for further selection. A total of 312 polymorphic loci detected by 9 AFLP marker combinations were used tomonitor the genetic diversity of the populations for the background selection. The B3C2 population of 51 clones wasfinally selected, of which the frequency of resistant genotypes increased by 23.8% points and the genetic diversity wasmaintained by about 96% as referred to B3C1. Our results strongly suggested that combination of the foregroundselection for target traits and the background selection for the genetic diversity is an efficient strategy in the recurrentselection of tetraploid potato to improve quantitative traits.
Keywords:  potato')" href="#">  
Received: 03 October 2010   Accepted:
Corresponding Authors:  Correspondence XIE Cong-hua, Professor, Tel: +86-27-87280969, Fax: +86-27-87286939, E-mail: xiech@mail.hzau.edu.cn   
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YAO Chun-guang
SONG Bo-tao
LIU Jun
WU Cheng-jin
CHENG Qun
LI Da-chun and XIE Conghua

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YAO Chun-guang, SONG Bo-tao, LIU Jun, WU Cheng-jin, CHENG Qun, LI Da-chun and XIE Conghua. 2011. null. Journal of Integrative Agriculture, 10(8): 1177-1187.

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