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| Differential Gene and Protein Expression in Soybean at Early Stages of Incompatible Interaction with Phytophthora sojae |
| LI Yong-gang1, YANG Ming-xiu1, LI Yan1, LIU Wen-wen1, WEN Jing-zhi1 and LI Yong-hao2 |
1 Department of Plant Protection, Northeast Agricultural University, Harbin 150030, P.R.China
2 Department of Plant Pathology and Ecology, Connecticut Agricultural Experiment Station, 123 Huntington St. New Haven, CT 06511, USA |
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摘要 Soybean root and stem rot caused by Phytophthora sojae is a destructive disease worldwide. Using genetic resistance is an important and major component in the integrated pest management of this disease. To understand molecular mechanisms of root and stem rot resistance in soybeans, the gene and protein expression in hypocotyls and stems of variety Suinong 10 carrying resistance genes Rps1a and Rps2 was investigated by using mRNA differential display reverse transcription PCR and two-dimensional electrophoresis at 0, 0.5, 1, 2, and 4 h after inoculation with P. sojae race 1. The results of the comparison of gene and protein expression showed that at least eight differential fragments at the transcriptional level were related to metabolic pathway, phytoalexin, and signal transduction in defense responses.Sequence analyses indicated that these fragments represented cinnamic acid 4-hydroxylase gene, ATP β gene coding ATP synthase β subunit and ubiquitin-conjugating enzyme gene which upregulated at 0.5 h post inoculation, blue copper protein gene and UDP-N-acetyl-α-D-galactosamine gene which upregulated at 2 h post inoculation, TGA-type basic leucine zipper protein TGA1.1 gene, cyclophilin gene, and 14-3-3 protein gene which upregulated at 4 h post inoculation.Three resistance-related proteins, α-subunit and β-subunit of ATP synthase, and cytochrome P450-like protein, were upregulated at 2 h post inoculation. The results suggested that resistance-related multiple proteins and genes were expressed in the recognition between soybean and P. sojae during zoospore germination, penetration and mycelium growth of P. sojae in soybean.
Abstract Soybean root and stem rot caused by Phytophthora sojae is a destructive disease worldwide. Using genetic resistance is an important and major component in the integrated pest management of this disease. To understand molecular mechanisms of root and stem rot resistance in soybeans, the gene and protein expression in hypocotyls and stems of variety Suinong 10 carrying resistance genes Rps1a and Rps2 was investigated by using mRNA differential display reverse transcription PCR and two-dimensional electrophoresis at 0, 0.5, 1, 2, and 4 h after inoculation with P. sojae race 1. The results of the comparison of gene and protein expression showed that at least eight differential fragments at the transcriptional level were related to metabolic pathway, phytoalexin, and signal transduction in defense responses.Sequence analyses indicated that these fragments represented cinnamic acid 4-hydroxylase gene, ATP β gene coding ATP synthase β subunit and ubiquitin-conjugating enzyme gene which upregulated at 0.5 h post inoculation, blue copper protein gene and UDP-N-acetyl-α-D-galactosamine gene which upregulated at 2 h post inoculation, TGA-type basic leucine zipper protein TGA1.1 gene, cyclophilin gene, and 14-3-3 protein gene which upregulated at 4 h post inoculation.Three resistance-related proteins, α-subunit and β-subunit of ATP synthase, and cytochrome P450-like protein, were upregulated at 2 h post inoculation. The results suggested that resistance-related multiple proteins and genes were expressed in the recognition between soybean and P. sojae during zoospore germination, penetration and mycelium growth of P. sojae in soybean.
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Received: 10 June 2011
Online: 10 June 2011
Accepted:
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Corresponding Authors:
WEN Jing-zhi
E-mail: neaulyg@yahoo.cn;jzhwen2000@yahoo.com.cn
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| About author: LI Yong-gang, Ph D, E-mail: neaulyg@yahoo.cn; Correspondence WEN Jing-zhi, Professor, Ph D, Tel: +86-451-55191045, Fax: +86-451-55191045, E-mail:
jzhwen2000@yahoo.com.cn |
Cite this article:
LI Yong-gang, YANG Ming-xiu, LI Yan, LIU Wen-wen, WEN Jing-zhi and LI Yong-hao.
2011.
Differential Gene and Protein Expression in Soybean at Early Stages of Incompatible Interaction with Phytophthora sojae. Journal of Integrative Agriculture, 10(6): 902-910.
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