摘要 Actin and myosin were found to be associated with the cytoplasmic sleeve of plasmodesmata. As cytoskeletal proteins, actin and myosin are believed to regulate the conductivity of plasmodesmata (PDs) in higher plants. Using immunocytochemical methods, we found the two proteins to be co-localized - and closely linked to each other - in plasmodesmata and ectodesmata-like structure in ageing parenchymatous cells of Allium sativum L. We suggest that intercellular communication is affected by the interaction between actin and myosin.
Abstract Actin and myosin were found to be associated with the cytoplasmic sleeve of plasmodesmata. As cytoskeletal proteins, actin and myosin are believed to regulate the conductivity of plasmodesmata (PDs) in higher plants. Using immunocytochemical methods, we found the two proteins to be co-localized - and closely linked to each other - in plasmodesmata and ectodesmata-like structure in ageing parenchymatous cells of Allium sativum L. We suggest that intercellular communication is affected by the interaction between actin and myosin.
DONG Yu, LIU Na, LIU Gang, LI Wen-long, YAN Ai-hua and WANG Dong-mei.
2011.
Actin and Myosin Co-Localize in Plasmodesmata and Ectodesmata-Like Structure. Journal of Integrative Agriculture, 10(6): 845-849.
Baluška F, Šamaj J, Napier R, Volkmann D. 1999. Maize calreticulin localizes preferentially to plasmodesmata in root apex. The Plant Journal, 19, 481-488.
Blackman L M, Overall R L. 1998. Immunolocalization of the cytoskeleton to plasmodesmata of Chara corallina. The Plant Journal, 14, 733-741.
Citovsky V, McLean B G, Zupan J, Zambryski P. 1993.Phosphorylation of tobacco mosaic virus cell-to-cell movement protein by a developmentally regulated plant cell wall-associated protein kinase. Genes & Development, 7, 904-910.
Ding B, Turgeon R, Parthasarathy M V. 1992. Substructure of freeze-substituted plasmodesmata. Protoplasma, 169, 28- 41.
Ding B, Kwon M O, Warnberg L. 1996. Evidence that actin filaments are involved in controlling the permeability of plasmodesmata in tobacco mesophyll. The Plant Journal, 10, 157-164.
Dong Y, Liu N, Wang Z H, Wang D M. 2009. The formation and symplastic transport function of ectodesmata-like in garlic during the parenchyma cells declining. Journal of Molecular Cell Biology, 42, 165-172. (in Chinese)
Heinlein M, Epel B L. 2004. Macromolecular transport and signaling through plasmodesmata. International Review of Cytology, 235, 93-164.
Jung G, Hammer III J A. 1994. The actin binding site in the tail domain of Dictyostelium myosin IC (myoC) resides within the glycine- and proline-rich sequence (tail homology region 2). FEBS Letters, 342, 197-202.
Lucas W J, Ding B, van der Schoot C. 1993. Tansley review no. 58 Plasmodesmata and the supracellular nature of plants. New Phytologist, 125, 435-476.
Oparka K J, Roberts A G, Boevink P, Santa C S, Roberts L, Pradel K S, Imlau A, Kotlizky G, Sauer N, et al. 1999. Simple, but not branched, plasmodesmata allow the nonspecific trafficking of proteins in developing tobacco leaves. Cell, 97, 743-754.
Pan Y F, Wang X Y, Zheng G C. 2002. The arrangement of microtrabecular network during chromatin migration process and immunolocalization of actin and myosin in pollen mother cells of David Lily. Caryologia, 55, 217-227.
Pollard T D, Doberstein S K, Zot H G. 1991. Myosin-I. Annual Review of Physiology, 53, 653-681.
Radford J E, White R G. 1998. Localization of a myosin-like protein to plasmodesmata. The Plant Journal, 14, 743-750. Robards A W, Lucas W J. 1990. Plasmodesmata. Annual Review of Plant Physiology and Plant Molecular Biology, 41, 369- 419.
Roberts A G, Oparka K J. 2003. Plasmodesmata and the control of symplastic transport. Plant, Cell & Environment, 26, 103- 124.
Wang D M, Wang X C, Zhang W C. 2002. Evidence of ultrastructure and physiology of F-actin as component of plasmodesmata. Acta Botanica Sinica, 44, 1278-1285.
Wang Z H, Liu N, Liu G, Wang D M. 2007. The structural characteristics and symplastic transport function of the ectodesmata-like of root cap tissue in Zea Mays. Journal of Molecular Cell Biology, 40, 351-358. (in Chinese)
White R G, Badelt K, Overall R L, Vesk M. 1994. Actin associated with plasmodesmata. Protoplasma, 180, 169-184.
Zhang W C, Yan W M. 1995. Detachment of root cap cells of maize and its effect on the relationship between root and rhizosphere. Acta Phytophysiologica Sinica, 21, 340-346. (in Chinese)
Zhang W C, Yan W M, Gao X Y. 1999. Cytological evidences of a possible mode of nurture absorption of the differentiating embryo in Triticum aestivum. Acta Botanica Sinica, 41, 373- 378. (in Chinese)
Zhang Y Y, Wang D M, Hou C Y, Han S F , Li X J, Liu G, Liu N. 2004. Study on the change of intercellular connection and the appearance and function of ectodesmata-like structure in garlic clove during the dormancy development. Acta Biologiae Experimentalis Sinica, 37, 212-220. (in Chinese)
