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| Angiopoietin-Like Protein 3 Expression is Down-Regulated in Experimentally Pregnant Toxemic Goats |
| CHEN Xiao-jun, BAI Xia, MAI Pei, CAI Jie, LIU Zhao-ying, WANG Hui, XIAO Hong-bo, DONG Wei, WANG Shui-lian, SUN Zhi-liang, |
1.College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, P.R.China
2.Engineering Research Center of Veterinary Drugs of Hunan Province, Changsha 410311, P.R.China |
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摘要 Pregnancy toxemia is a metabolic disorder of lipid and glucose. Recent investigations have found that angiopoietin-like protein 3 (ANGPTL3) can contribute to disorder of carbohydrate and lipid metabolism. The present study was conducted to investigate the change of ANGPTL3 expression during pregnancy toxemia. We firstly cloned the full-length cDNA of ANGPTL3 in Liuyang Black goats, revealing that goat ANGPTL3 had the typical structure of the angiopoietin-like family, and its mRNA was exclusively expressed in liver. Pregnancy toxemia of pregnant goat does with twins during late gestation was induced by being fasted for 72 h, and then they were recovered after 5 d of refeeding. Hepatic ANGPTL3 gene expression was significantly down-regulated concomitantly with decreased serum glucose concentration, elevated serum β-hydroxybutyrate and free fatty acid levels in pregnant toxemic goats, and these changes were reversed after refeeding. These results suggest ANGPTL3 may play a certain role in the development of pregnancy toxemia in goats.
Abstract Pregnancy toxemia is a metabolic disorder of lipid and glucose. Recent investigations have found that angiopoietin-like protein 3 (ANGPTL3) can contribute to disorder of carbohydrate and lipid metabolism. The present study was conducted to investigate the change of ANGPTL3 expression during pregnancy toxemia. We firstly cloned the full-length cDNA of ANGPTL3 in Liuyang Black goats, revealing that goat ANGPTL3 had the typical structure of the angiopoietin-like family, and its mRNA was exclusively expressed in liver. Pregnancy toxemia of pregnant goat does with twins during late gestation was induced by being fasted for 72 h, and then they were recovered after 5 d of refeeding. Hepatic ANGPTL3 gene expression was significantly down-regulated concomitantly with decreased serum glucose concentration, elevated serum β-hydroxybutyrate and free fatty acid levels in pregnant toxemic goats, and these changes were reversed after refeeding. These results suggest ANGPTL3 may play a certain role in the development of pregnancy toxemia in goats.
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Received: 06 December 2011
Accepted:
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| Fund: This work was supported by the National Natural Science Foundation of China (31072167), the Key Project of Hunan Provincial Science & Technology Department, China (2009FJ1005), and Innovation Project of Hunan Provincial Education Department (CX2009B153), China. |
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Corresponding Authors:
SUN Zhi-liang, Tel/Fax: +86-731-84635229, E-mail: sunzhiliang1965@yahoo.com.cn
E-mail: sunzhiliang1965@yahoo.com.cn
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| About author: CHEN Xiao-jun, Mobile: 13787116323, E-mail: xj92e2@163.com; |
Cite this article:
CHEN Xiao-jun, BAI Xia, MAI Pei, CAI Jie, LIU Zhao-ying, WANG Hui, XIAO Hong-bo, DONG Wei, WANG Shui-lian, SUN Zhi-liang, .
2012.
Angiopoietin-Like Protein 3 Expression is Down-Regulated in Experimentally Pregnant Toxemic Goats. Journal of Integrative Agriculture, 12(7): 1181-1188.
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| [1]Ando Y, Shimizugawa T, Takeshita S, Ono M, Shimamura M, Koishi R, Furukawa H. 2003. A decreased expression of angiopoietin-like 3 is protective against atherosclerosis in apoE-deficient mice. Journal of Lipid Research, 44, 1216-1223. [2]Andrews A H, Holland-Hoewes V E, Wilkinson J I D. 1996. Naturally occurring pregnancy toxemia in the ewe and treatment with recombinant bovine somatotropin. Small Ruminant Research, 23, 191-197. [3]Brozos C, Mavrogianni V S, Fthenakis G C. 2011. Treatment and control of peri-parturient metabolic diseases: pregnancy toxemia, hypocalcemia, hypomagnesemia. Veterinary Clinics of North America-Food Animal Practice, 27, 105-113. [4]Conklin D, Gilbertson D, Taft D W, Maurer M F, Whitmore T E, Smith D L, Walker K M, Chen L H, Wattler S, Nehls M, et al. 1999. Identification of a mammalian angiopoietin-related protein expressed specifically in liver. Genomics, 62, 477-482. [5]van Dorland H A, Richter S, Morel I, Doherr M G, Castro N, Bruckmaier R M. 2009. Variation in hepatic regulation of metabolism during the dry period and in early lactation in dairy cows. Journal of Dairy Science, 92, 1924-1940. [6]Feng S Q, Chen X D, Xia T, Gan L, Qiu H, Dai M H, Zhou L, Peng Y, Yang Z Q. 2006. Cloning, chromosome mapping and expression characteristics of porcine ANGPTL3 and-4. Cytogenetic and Genome Research, 114, 44-49. [7]Fujimoto K, Koishi R, Shimizugawa T, Ando Y. 2006. Angptl3-null mice show low plasma lipid concentrations by enhanced lipoprotein lipase activity. Experimental Animals, 55, 27-34. [8]Heitmann R N, Dawes D J, Sensenig S C. 1987. Hepatic ketogenesis and peripheral ketone body utilization in the ruminant. Journal of Nutrition, 117, 1174-1180. [9]Inukai K, Nakashima Y, Watanabe M, Kurihara S, Awata T, Katagiri H, Oka Y, Katayama S. 2004. ANGPTL3 is increased in both insulin-deficient and-resistant diabetic states. Biochemical and Biophysical Research Communications, 317, 1075-1079. [10]Ismail Z A, Al-Majali A M, Amireh F, Al-Rawashdeh O F. 2008. Metabolic profiles in goat does in late pregnancy with and without subclinical pregnancy toxemia. Veterinary Clinical Pathology, 37, 434-437. [11]Kim I, Kim H G, Kim H, Kim H H, Park S K, Uhm C S, Lee Z H, Koh G Y. 2000. Hepatic expression, synthesis and secretion of a novel fibrinogen/angiopoietin-related protein that prevents endothelial-cell apoptosis. Biochemical Journal, 346, 603-610. [12]Koishi R, Ando Y, Ono M, Shimamura M, Yasumo H, Fujiwara T, Horikoshi H, Furukawa H. 2002. Angptl3 regulates lipid metabolism in mice. Nature Genetics, 30, 151-157. [13]Kronfeld D S. 1972. Ketosis in pregnant sheep and lactating cows. Australian Veterinary Journal, 48, 680-687. [14]Li C. 2006. Genetics and regulation of angiopoietin-like proteins 3 and 4. Current Opinion in Lipidology, 17, 152-156. [15]Nakajima K, Kobayashi J, Mabuchi H, Nakano T, Tokita Y, Nagamine T, Imamura S, Ai M, Otokozawa S, Schaefer E F. 2010. Association of angiopoietin-like protein 3 with hepatic triglyceride lipase and lipoprotein lipase activities in human plasma. Annals of Clinical Biochemistry, 47, 423-431. [16]Oike Y, Akao M, Kubota Y, Suda T. 2005. Angiopoietin-like proteins: potential new targets for metabolic syndrome therapy. Trends in Molecular Medicine, 11, 473-479. [17]Robciuc M R, Tahvanainen E, Jauhiainen M, Ehnholm C. 2010. Quantitation of serum angiopoietin-like proteins 3 and 4 in a Finnish population sample. Journal of Lipid Research, 51, 824-831. [18]Rook J S. 2000. Pregnancy toxaemia of ewes, does, and beef cows. Veterinary Clinics of North America: Food Animal Practice, 16, 293-317. [19]Sato K, Seol H S, Sato K, Kamada T, Akiba Y. 2008. Molecular characterization and expression of angiopoietin-like protein 3 in the chicken, Gallus gallus. General and Comparative Endocrinology, 158, 102-107. [20]van Saun R J. 2000. Pregnancy toxemia in a flock of sheep. Journal of the American Veterinary Medical Association, 217, 1536-1539. [21]Shimamura M, Matsuda M, Ando Y, Koishi R, Yasumo H, Furukawa H, Shimomura I. 2004. Leptin and insulin down-regulate angiopoietin-like protein 3, a plasma triglyceride-increasing factor. Biochemical and Biophysical Research Communications, 322, 1080-1085. [22]Shimamura M, Matsuda M, Kobayashi S, Ando Y, Ono M, Koishi R, Furukawa H, Makishima M, Shimomura I. 2003. Angiopoietin-like protein 3, a hepatic secretory factor, activates lipolysis in adipocytes. Biochemical and Biophysical Research Communications, 301, 604-609. [23]Shimamura M, Matsuda M, Yasumo H, Okazaki M, Fujimoto K, Kono K, Shimizugawa T, Ando Y, Koishi R, Kohama T, et al. 2007. Angiopoietin-like protein 3 regulates plasma HDL cholesterol through suppression of endothelial lipase. Arteriosclerosis Thrombosis and Vascular Biology, 27, 366-372. [24]Shimizugawa T, Ono M, Shimamura M, Yoshida K, Ando Y, Koishi R, Ueda K, Inaba T, Minekura H, Kohama T, et al. 2002. ANGPTL3 decreases very low density lipoprotein triglyceride clearance by inhibition of lipoprotein lipase. The Journal of Biological Chemistry, 277, 33742-33748. [25]Shoji T, Hatsuda S, Tsuchikura S, Kimoto E, Kakiya R, Tahara H, Koyama H, Emoto M, Tabata T, Nishizawa Y. 2009. Plasma angiopoietin-like protein 3 (ANGPTL3) concentration is associated with uremic dyslipidemia. Atherosclerosis, 207, 579-584. [26]Varnam G C, Jeacock M K, Shepherd D A. 1978. Hepatic ketene-body metabolism in developing sheep and pregnant ewes. British Journal of Nutrition, 40, 359-367. [27]West H J. 1996. Maternal undernutrition during late pregnancy in sheep. Its relationship to maternal condition, gestation length, hepatic physiology and glucose metabolismp. British Journal of Nuirition, 26, 12-16. [28]Yarim G F, Karahan S, Nisbet C. 2007. Elevated plasma levels of interleukin 1 beta, tumour necrosis factor alpha and monocyte chemotactic protein 1 are associated with pregnancy toxaemia in does. Veterinary Research Communication, 31, 565-573. |
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