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Journal of Integrative Agriculture  2012, Vol. 12 Issue (7): 1159-1166    DOI: 10.1016/S1671-2927(00)8642
ANIMAL SCIENCE · VETERINARY SCIENCE Advanced Online Publication | Current Issue | Archive | Adv Search |
Hoxc13/β-catenin Correlation with Hair Follicle Activity in Cashmere Goat
 WU Jiang-hong, ZHANG Yan-jun, ZHANG Jia-xin, CHANG Zi-li, LI Jin-quan, YAN Zu-wei, Husile ,  ZHANG Wen-guang
1.Key Laboratory of Animal Genetics, Breeding and Reproduction, Inner Mongolian Autonomous Region, Hohhot 010018, P.R.China
2.College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, P.R.China
3.College of Science, Inner Mongolia Agricultural University, Huhhot 010018, P.R.China
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摘要  Seasonal hair follicle activity and fibre growth in some Cashmere-bearing goats (Caprus hircus) is a cyclic process that is well characterized morphologically but understood incompletely at the molecular level. As an initial step in discovering regulators in hair-follicle activity and cycling, we used qPCR to investigate 19 genes expression in Cashmere goat side skin from 12 mon. Many of these genes may be associated with the hair follicle development-relevant genes (HFDRGs) in the literature. Here we show that Hoxc13/β-catenin gene associated with the follicle activity. In addition, Hoxc13 was found to be expressed with an drastic increase between July and November for melatonin treatments. To further investigate the role of Hoxc13 on HFDRGs, fibroblasts and keratinocytes from Cashmere goat skin were transfected with p-ECFPHoxc13. The result suggested that overexpression of Hoxc13 gene decreased HFDRGs with negative role for hair follicle development and increase HFDRGs with positive role for hair follicle development in vitro. These findings provide data on the Hoxc13 expression profile of normal Cashmere goat skin and Cashmere goat skin with melatonin treatment, and demonstrate hair-follicle-activity dependent regulation of Hoxc13 expression.

Abstract  Seasonal hair follicle activity and fibre growth in some Cashmere-bearing goats (Caprus hircus) is a cyclic process that is well characterized morphologically but understood incompletely at the molecular level. As an initial step in discovering regulators in hair-follicle activity and cycling, we used qPCR to investigate 19 genes expression in Cashmere goat side skin from 12 mon. Many of these genes may be associated with the hair follicle development-relevant genes (HFDRGs) in the literature. Here we show that Hoxc13/β-catenin gene associated with the follicle activity. In addition, Hoxc13 was found to be expressed with an drastic increase between July and November for melatonin treatments. To further investigate the role of Hoxc13 on HFDRGs, fibroblasts and keratinocytes from Cashmere goat skin were transfected with p-ECFPHoxc13. The result suggested that overexpression of Hoxc13 gene decreased HFDRGs with negative role for hair follicle development and increase HFDRGs with positive role for hair follicle development in vitro. These findings provide data on the Hoxc13 expression profile of normal Cashmere goat skin and Cashmere goat skin with melatonin treatment, and demonstrate hair-follicle-activity dependent regulation of Hoxc13 expression.
Keywords:  Hoxc13      β-catenin      Cashmere goat      hair follicle activity      cycle  
Received: 19 January 2011   Accepted:
Fund: 

This research was conducted with financial support from the National Natural Science Foundation of China (30960246), the Key Project of National Science and Technology Pillar Program of China (2011BAD28B05), the National High Technology Research and Development Program of China (2007AA10Z151), the Specialized Research Fund for the Doctoral Program of Higher Education (20091515120010), the Inner Mongolia Natural Science Foundation, China (20080404ZD04), and the China Agriculture Research System (CARS-40).

Corresponding Authors:  ZHANG Wen-guang, E-mail: atcgnmbi@yahoo.com.cn; LI Jin-quan, Mobile: 15047996076,E-mail: lijinquan_nd@126.com     E-mail:  atcgnmbi@yahoo.com.cn
About author:  WU Jiang-hong, E-mail: wujianghonglong@126.com

Cite this article: 

WU Jiang-hong, ZHANG Yan-jun, ZHANG Jia-xin, CHANG Zi-li, LI Jin-quan, YAN Zu-wei, Husile , ZHANG Wen-guang . 2012. Hoxc13/β-catenin Correlation with Hair Follicle Activity in Cashmere Goat. Journal of Integrative Agriculture, 12(7): 1159-1166.

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