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| Preparation, Characterization and Nematicidal Activity of Lansiumamide B Nano-Capsules |
| YIN Yan-hua, GUO Qing-ming, HAN Yun, WANG Ling-jing, WAN Shu-qing |
| Key Laboratory of Natural Pesticide & Chemical-Biology of Education Ministry/South China Agricultural University, Guangzhou 510642,P.R.China |
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摘要 In this study, nano-capsules of lansiumamide B (NCLB) was prepared by the microemulsion polymerization method to improve the nematicidal efficacy of lansiumamide B (LB). An optimal formulation was gained by orthogonal experiment design based on the encapsulation efficiency (En, %) value. The optimized NCLB were spherical and uniform under transmission electron microscopy (TEM). The mean particle size, zeta potential and En were (38.50±0.64) nm, (-70.5±0.76) mV and (95.13±1.16)%, respectively. The release profile indicated that the accumulated release of LB in NCLB reached up to 82% within 96 h. Effects of NCLB against Bursaphelenehus xylophilus and J2 of Meloidogyne incognita were reported in this paper. The nematicidal activity of NCLB has been remarkably increased, with LC50 values of 2.1407 mg L-1 and 19.3608 mg L-1, respectively, at 24 h after treatment. The disease progression and the average number of root knots of Ipomoea aquatica were 1.50 and 7.25, respectively, in the treatment of NCLB, at concentration of 200 mg L-1, significantly lower than the treatment of LB and ethoprophos. Compared to control, the treatments of NCLB, LB and ethoprophos leaded the disease progression to drop 68.42, 36.84 and 26.32%, respectively, and caused the average number of root knots to fall 83.94, 78.03 and 63.66%. These results suggested that NCLB, as a novel nematicides formulation, performed more efficient and longer effective maintenance against plant parasitic nematodes.
Abstract In this study, nano-capsules of lansiumamide B (NCLB) was prepared by the microemulsion polymerization method to improve the nematicidal efficacy of lansiumamide B (LB). An optimal formulation was gained by orthogonal experiment design based on the encapsulation efficiency (En, %) value. The optimized NCLB were spherical and uniform under transmission electron microscopy (TEM). The mean particle size, zeta potential and En were (38.50±0.64) nm, (-70.5±0.76) mV and (95.13±1.16)%, respectively. The release profile indicated that the accumulated release of LB in NCLB reached up to 82% within 96 h. Effects of NCLB against Bursaphelenehus xylophilus and J2 of Meloidogyne incognita were reported in this paper. The nematicidal activity of NCLB has been remarkably increased, with LC50 values of 2.1407 mg L-1 and 19.3608 mg L-1, respectively, at 24 h after treatment. The disease progression and the average number of root knots of Ipomoea aquatica were 1.50 and 7.25, respectively, in the treatment of NCLB, at concentration of 200 mg L-1, significantly lower than the treatment of LB and ethoprophos. Compared to control, the treatments of NCLB, LB and ethoprophos leaded the disease progression to drop 68.42, 36.84 and 26.32%, respectively, and caused the average number of root knots to fall 83.94, 78.03 and 63.66%. These results suggested that NCLB, as a novel nematicides formulation, performed more efficient and longer effective maintenance against plant parasitic nematodes.
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Received: 07 September 2011
Accepted:
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| Fund: This work was supported by Guangzhou Science and Technology Plan Projects, Guangzhou, China (10C62101593). |
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Corresponding Authors:
WAN Shu-qing, Tel: +86-20-85285813, E-mail: wanshuqing1953@yahoo.com.cn
E-mail: wanshuqing1953@yahoo.com.cn
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| About author: YIN Yan-hua, Mobile: 13544495468, E-mail: yinyanhua1986000@163.com |
Cite this article:
YIN Yan-hua, GUO Qing-ming, HAN Yun, WANG Ling-jing, WAN Shu-qing.
2012.
Preparation, Characterization and Nematicidal Activity of Lansiumamide B Nano-Capsules. Journal of Integrative Agriculture, 12(7): 1151-1158.
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| [1]Chang C P, Takao Y, Miho K, Takashi S, Kimio I, Toshioki D. 2003. Release characteristics of an azo dye from poly (ureaurethane) microcapsules. Journal of Controlled Release, 86, 207-211. [2]Feczkó T, Vargad O, Kovácse M, Vidóczyd T, Voncina B. 2011. Preparation and characterization of photochromic poly (methyl methacrylate) and ethyl cellulose nanocapsules containing a spirooxazine dye. Journal of Photochemistry and Photobiology (A: Chemistry), 222, 293-298. [3]Ghorbani R, Wilcockson S, Koochek A, Leifert C. 2008. Soil management for sustainable crop disease control: a review. Environmental Chemistry Letters, 6, 149-162. [4]Guo H L, Zhao X P. 2003. Preparation and application of nanocapsule. Function Materials, 6, 609-611. (in Chinese) [5]Han M J, Lee E H, Kim E Y. 2002. Preparation and optical properties of polystyrene nanocapsules containing photochromophores. Optical Materials, 21, 579-583. [6]Han Z R, Du Y C, Li G, Mu W, Liu F. 2007. Preparation and sustained release behavior of abamectin ureaformaldehyde resin microcapsules. Chinese Journal of Pesticide Science, 9, 405-410. (in Chinese) [7]Hureaux J, Lagarce F, Gagnadoux F, Clavreul A, Benoit J P, Urban T. 2009. The adaptation of lipid nanocapsule formulations for blood administration in animals. International Journal of Pharmaceutics, 379, 266-269. [8]Hynes R K, Boyetchko S M. 2006. Research initiatives in the art and science of biopesticide formulations. Soil Biology and Biochemistry, 38, 845-849. [9]Jafaria A H, Hosseinib S M A, Jamalizadeh E. 2010. Investigation of smart nanocapsules containing inhibitors for corrosion protection of copper. Electrochimica Acta, 55, 9004-9009. [10]Jega T C, Taverdet J L. 2000. Stirring speed influence study on the microencapsulation process and on the drug release from microcapsules. Polymer Bulletin, 44, 345-351. [11]Jia Z R, Lin P, Xiang Y, Wang X Q, Wang J C, Zhang X, Zhang Q. 2011. A novel nanomatrix system consisted of colloidal silica and pH-sensitive polymethylacrylate improves the oral bioavailability of fenofibrate. European Journal of Pharmaceutics and Biopharmaceutics, 79, 126-134. [12]Kitajima M, Butler W L. 1976. Microencapsulation of chloroplast particles. Plant Physiology, 57, 746-750. [13]Lamberti F, Taylor C E. 1979. Root-Knot Nematodes (Meloidogyne Species): Systematics, Biology and Control. Academic Press, New York, USA. [14]Li Z Z, Wen L X, Liu F, Liu A Q, Wang Q, Chen J F. 2005. Fabrication of novel avermectin-porous hollow silica nanoparticles (Av-PHSN) carrier and its properties. Chinese Journal of Pesticide Science, 7, 275-281. (in Chinese) [15]Lin J H. 1989. Cinnamamide derivatives from clausena lansium. Phytochemistry, 28, 621-622. [16]Liu W Z. 2000. Plant Pathogen Nematology. China Agriculture Press, Beijing, China. pp. 58-243. (in Chinese) [17]Liu Y X, Gong Z Y, Wang S Q. 2009. Inhibitory effects of clausenamide alkaloid on seven fruit pathogenic fungi. Plant Protection, 35, 53-56. (in Chinese) [18]Ma F N, Wan S Q, Liu X M, Zhao F. 2009. Active ingredients of seed extracts of clausena lansium and nematocidal activity against Bursaphelenchus xylophilus. Journal of South China Agricultural University, 30, 23-26. (in Chinese) [19]Manzanilla-Lopez R H, Kenneth E, Bridge J. 2004. Plant diseases caused by nematodes. In: Chen Z X, Chen S Y, Dickson D W, eds., Nematology-Advances and Perspectives. Volume II: Nematode Management and Utilization. CABI Publishing, Cambridge, MA. pp. 637-716. [20]Mohanraj V J, Barnes T J, Prestidge C A. 2010. Slica nanoarticle coated liposomes: A new type of hybrid nanocapsule for proteins. International Journal of Pharmaceutics, 392, 285-293. [21]Ning H. 2009. The latest progress of nano measurement technology light scattering. Malvern Instruments Ltd. [2011-05-20]. http://www.malvern.com/ [22]Oka Y, Shuker S, Tkachi N. 2009. Nematicidal efficacy of MCW-2, a new nematicide of the fluoroalkenyl group, against the root-knot nematode Meloidogyne javanica. Pest Management Science, 65, 1082-1089. [23]Regulation EC No 396/2005 of the European Parliament and of the Council of Europe. 2005. [2010-09-15]. http:/ /eur-lex.europa.eu/ [24]Regulation EC No 1095/2007 of the European Parliament and of the Council of Europe. 2007. [2010-09-15]. http:/ /eur-lex.europa.eu/ [25]Regulation EC No 33/2008 of the European Parliament and of the Council of Europe. 2008. [2010-09-15]. http://eurlex. europa.eu/ [26]Shang Q, Feng S B, Zheng H T. 2006. Preparation of abamectin-nanocapsules suspension concentrate. Agrochemicals, 12, 831-833. (in Chinese) [27]Shang Q, Shang Z H, Ci S Y. 2007. HPLC analysis of abamectin-nanocapsules suspension concentrate. Agrochemicals, 46, 185-186. (in Chinese) [28]Sharon E, Chet I, Viterbo A, Bar-Eyal M, Nagan H, Samuels G J, Spiegel Y. 2007. Parasitism of Trichoderma on Meloidogyne javanicaand role of the gelatinous matrix. European Journal of Plant Pathology, 118, 247-258. [29]Sun C M, Zhang S X, Li C S, Yin Y S. 2000. Study progress of microemulsion polymerization. China Powder Science and Technology, 6, 28-31. (in Chinese) [30]Suzuki K, Wakatuki Y, Shirasaki S, Fujita K, Kato S, Nomura M. 2005. Effect of mixing ratio of anionic and nonionic emulsifiers on the kinetic behavior of methyl methacrylate emulsion polymerization. Polymer, 46, 5890-5895. [31]Thorne G. 1961. Principles of Nematology. McGraw-Hill Book Company Inc., New York, USA. p. 553. Trudgill D L, Blok V C 2001. Apomictic, polyphagous rootknot nematodes: exceptionally successful and damaging biotrophic root pathogens. Annual Review of Phytopathology, 39, 53-77. [32]Uri N D. 1998. Development and use of biopesticides: implications of government policy and consumers’ preferences. Technological Forecasting and Social Change, 59, 291-304. [33]Wang Z, Ho P C. 2010. A nanocapsular combinatorial sequential drug delivery system for antiangiogenesis and anticancer activities. Biomaterials, 31, 7115-7123. [34]Weng Q F, Zhong G H, Qiu Q, Shui K J, Hu M Y. 2005. Biological activities of extracts of 28 species of plants against Bursaphelenchus xylophilus. Journal of Huazhong Agricultural University (China), 24, 459-464. (in Chinese) [35]Weng Q F, Zhong G H, Wang W X, Luo J J, Hu M Y. 2006. Effectiveness of plant extracts for the control of meloidogyne incognita. Journal of South China Agricultural University, 27, 55-60. (in Chinese) [36]Wu H P, Xu X L, Wang J. 2007. In vitro nematicidal activity of ethanol plant extracts of tea seed against pine-wood nematode. Plant Quarantine, 21, 335-337. (in Chinese) [37]Wu J, Chen J, Zhou Y F, Nie W Y, Cheng X M. 2007. Structure characterization and insecticidal activity tests of natural pyrethrin and abermectin nanocapsules. Agrochemicals, 46, 672-674. (in Chinese) [38]Wu J, Zhou Y F, Chen J, Nie W Y, Shi R. 2008. Preparation of natural pyrethrum nanocapsule by means of microemulsion. Polymerization Polymer Materials Science and Engineering, 24, 35-38. (in Chinese) [39]Yang B J, Tang J, Wang Y Y, Pang H Y, Wang L F. 2003. Pine Wilt Disease. Chinese Forest Publishing House Press, Beijing. (in Chiense) Yi C F, Zhou Z Q, Xu Z S. 2006. Research progress of microemulsion polymerization. Splice, 27, 31-34. [40]Yin X D, Zhang Z Y. 2010a. Preparation, properties and characteristics of azadirachtin/carboxymethyl chitosan/ phosphorylation chitosan nanoparticles. Acta Agriculturae Jiangxi, 22, 69-73. (in Chinese) [41]Yin X D, Zhang Z Y. 2010b. Preparation and characterization of matrine/carboxymethyl chitosan/phosphorylation chitosan nanoparticles. Materials Review, 24, 17-21. (in Chinese) [42]Zhou J, Chen T, Pu J, Wang X Y, Zheng B Z. 2007. A comparative study of controlled-release capability of microcapsules. Journal of Yuxi Teachers College, 23, 68-70. (in Chinese) [43]Zhu Y Y, Zhang G Y, Hong X L, Yang H Q. 2003. Progress on preparation of nanocapsules in colloid systems. Daily Chemical Industry, 33, 379-382. (in Chinese) |
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