Molecular Diversity and Association Analysis of Drought and Salt Tolerance in Gossypium hirsutum L. Germplasm

doi:10.1016/S2095-3119(13)60668-1

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (8): 1845-1853.DOI: 10.1016/S2095-3119(13)60668-1

• 论文 • 上一篇

Molecular Diversity and Association Analysis of Drought and Salt Tolerance in Gossypium hirsutum L. Germplasm

JIA Yin-hua, SUN Jun-ling, WANG Xi-wen, ZHOU Zhong-li, PAN Zao-e, HE Shou-pu, PANG Bao-yin, WANG Li-ru , DU Xiong-ming

State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, P.R.China

收稿日期:2013-06-26 出版日期:2014-09-22 发布日期:2014-09-24
通讯作者: DU Xiong-ming, E-mail: dujeffrey8848@hotmail.com
作者简介:JIA Yin-hua, E-mail: jia.yinhua@gmail.com
基金资助:
This research was supported by the National Natural Science Foundation of China (31201246) and the Project of International Science and Technology Cooperation and Exchange from the Ministry of Science and Technology, China (2010DFR30620-3).

Molecular Diversity and Association Analysis of Drought and Salt Tolerance in Gossypium hirsutum L. Germplasm

JIA Yin-hua, SUN Jun-ling, WANG Xi-wen, ZHOU Zhong-li, PAN Zao-e, HE Shou-pu, PANG Bao-yin, WANG Li-ru , DU Xiong-ming

State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, P.R.China

Received:2013-06-26 Online:2014-09-22 Published:2014-09-24
Contact: DU Xiong-ming, E-mail: dujeffrey8848@hotmail.com
About author:JIA Yin-hua, E-mail: jia.yinhua@gmail.com
Supported by:
This research was supported by the National Natural Science Foundation of China (31201246) and the Project of International Science and Technology Cooperation and Exchange from the Ministry of Science and Technology, China (2010DFR30620-3).

摘要/Abstract

摘要： Association mapping is a useful tool for the detection of genes selected during plant domestication based on their linkage disequilibrium (LD). This study was carried out to estimate genetic diversity, population structure and the extent of LD to develop an association framework in order to identify genetic variations associated with drought and salt tolerance traits. 106 microsatellite marker primer pairs were used in 323 Gossypium hirsutum germplasms which were grown in the drought shed and salt pond for evaluation. Polymorphism (PIC=0.53) was found, and three groups were detected (K=3) with the second likelihood ΔK using STRUCTURE software. LD decay rates were estimated to be 13-15 cM at r2 0.20. Significant associations between polymorphic markers and drought and salt tolerance traits were observed using the general linear model (GLM) and mixed linear model (MLM) (P 0.01). The results also demonstrated that association mapping within the population structure as well as stratification existing in cotton germplasm resources could complement and enhance quantitative trait loci (QTLs) information for marker-assisted selection.

关键词: cotton germplasm , genetic diversity , simple sequence repeats (SSR) markers , linkage disequilibrium (LD) , association analysis

Abstract: Association mapping is a useful tool for the detection of genes selected during plant domestication based on their linkage disequilibrium (LD). This study was carried out to estimate genetic diversity, population structure and the extent of LD to develop an association framework in order to identify genetic variations associated with drought and salt tolerance traits. 106 microsatellite marker primer pairs were used in 323 Gossypium hirsutum germplasms which were grown in the drought shed and salt pond for evaluation. Polymorphism (PIC=0.53) was found, and three groups were detected (K=3) with the second likelihood ΔK using STRUCTURE software. LD decay rates were estimated to be 13-15 cM at r2 0.20. Significant associations between polymorphic markers and drought and salt tolerance traits were observed using the general linear model (GLM) and mixed linear model (MLM) (P 0.01). The results also demonstrated that association mapping within the population structure as well as stratification existing in cotton germplasm resources could complement and enhance quantitative trait loci (QTLs) information for marker-assisted selection.

Key words: cotton germplasm , genetic diversity , simple sequence repeats (SSR) markers , linkage disequilibrium (LD) , association analysis

JIA Yin-hua, SUN Jun-ling, WANG Xi-wen, ZHOU Zhong-li, PAN Zao-e, HE Shou-pu, PANG Bao-yin, WANG Li-ru , DU Xiong-ming. Molecular Diversity and Association Analysis of Drought and Salt Tolerance in Gossypium hirsutum L. Germplasm[J]. Journal of Integrative Agriculture, 2014, 13(8): 1845-1853.

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Molecular Diversity and Association Analysis of Drought and Salt Tolerance in Gossypium hirsutum L. Germplasm

Molecular Diversity and Association Analysis of Drought and Salt Tolerance in Gossypium hirsutum L. Germplasm

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