Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (12): 2020-2027.DOI: 10.1016/S1671-2927(00)8739

• 论文 • 上一篇    下一篇

Genomic Analysis of the Natural Population of Wheat dwarf virus inWheat fromChina and Hungary

 LIU Yan, WANG Biao, Gyula Vida, Mónika Cséplo-Károlyi, WU Bei-lei, WU Yuan-hua, WANG Xifeng   

  1. 1 College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, P.R.China
    2 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
    3 Agricultural Research Institute, Hungarian Academy of Sciences, Martonvásár H-2462, Hungary
  • 收稿日期:2012-04-01 出版日期:2012-12-01 发布日期:2012-12-18
  • 通讯作者: Correspondence WANG Xi-feng, Tel: +86-10-62815928, Fax: +86-10-62815609, E-mail: xfwang@ippcaas.cn
  • 基金资助:

    This work was supported by the Inter-Governmental Agreement in Science and Technology (Sino-Hungary 333/2008 and CN- 13/2007) and the National Natural Science Foundation of China (30900938).

Genomic Analysis of the Natural Population of Wheat dwarf virus inWheat fromChina and Hungary

 LIU  Yan, WANG  Biao, Gyula  Vida, Mónika  Cséplo-Károlyi, WU  Bei-lei, WU  Yuan-hua, WANG  Xifeng   

  1. 1 College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, P.R.China
    2 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
    3 Agricultural Research Institute, Hungarian Academy of Sciences, Martonvásár H-2462, Hungary
  • Received:2012-04-01 Online:2012-12-01 Published:2012-12-18
  • Contact: Correspondence WANG Xi-feng, Tel: +86-10-62815928, Fax: +86-10-62815609, E-mail: xfwang@ippcaas.cn
  • Supported by:

    This work was supported by the Inter-Governmental Agreement in Science and Technology (Sino-Hungary 333/2008 and CN- 13/2007) and the National Natural Science Foundation of China (30900938).

PDF

摘要： During the last decade, the leafhopper transmitted Wheat dwarf virus (WDV) has become a serious problem both in northwestern China and Hungary. In order to study the molecular diversity and population structure of WDV in these two countries, 39 Chinese isolates and 16 Hungarian isolates were collected from different regions of China and Hungary, and their genomes were sequenced. All isolates belonged to the wheat strain of WDV and showed limited genetic diversity. The highest and lowest nucleotide sequence identities among isolates from China and Hungary were 99.9 and 90%, respectively. In all isolates, the lowest nucleotide sequence identity was 89.5% between MO10-1 and KP10-5, which were collected from Martonvásár and Kompolt, Hungary. Phylogenetic analyses showed the genome sequences of 55 WDV isolates belong to two big clades, but no clear correlation to geographical location. Population difference analyses indicated that the Chinese and Hungarian WDV populations have no significant difference. The regions in WDV genome with relatively low nucleotide diversities represented protein coding regions suggested that these regions evolved under negative selection, and might be one of the causes restricting the number of genetic variants.

关键词: geminivirus, Mastrevirus, phylogeny, genetic diversity

Abstract: During the last decade, the leafhopper transmitted Wheat dwarf virus (WDV) has become a serious problem both in northwestern China and Hungary. In order to study the molecular diversity and population structure of WDV in these two countries, 39 Chinese isolates and 16 Hungarian isolates were collected from different regions of China and Hungary, and their genomes were sequenced. All isolates belonged to the wheat strain of WDV and showed limited genetic diversity. The highest and lowest nucleotide sequence identities among isolates from China and Hungary were 99.9 and 90%, respectively. In all isolates, the lowest nucleotide sequence identity was 89.5% between MO10-1 and KP10-5, which were collected from Martonvásár and Kompolt, Hungary. Phylogenetic analyses showed the genome sequences of 55 WDV isolates belong to two big clades, but no clear correlation to geographical location. Population difference analyses indicated that the Chinese and Hungarian WDV populations have no significant difference. The regions in WDV genome with relatively low nucleotide diversities represented protein coding regions suggested that these regions evolved under negative selection, and might be one of the causes restricting the number of genetic variants.

Key words: geminivirus, Mastrevirus, phylogeny, genetic diversity