Association analysis of grain traits with SSR markers between Aegilops tauschii and hexaploid wheat (Triticum aestivum L.)

doi:10.1016/S2095-3119(15)61070-X

Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (10): 1936-1948.DOI: 10.1016/S2095-3119(15)61070-X

Association analysis of grain traits with SSR markers between Aegilops tauschii and hexaploid wheat (Triticum aestivum L.)

ZHAO Jing-lan, WANG Hong-wei, ZHANG Xiao-cun, DU Xu-ye, LI An-fei, KONG Ling-rang

1、State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, P.R.China
2、Taishan Polytechnic, Tai’an 271000, P.R.China

收稿日期:2014-10-24 出版日期:2015-10-09 发布日期:2015-10-12
通讯作者: KONG Ling-rang, Tel: +86-538-8249278,E-mail: lkong@sdau.edu.cn
作者简介:ZHAO Jing-lan, Tel: +86-538-8628134, E-mail: zhaojlan@sina. com;
基金资助:
We acknowledge financial supports by the National 973 Program of China (2014CB138100), the National Natural Science Foundation of China (31171553, 31471488 and 31200982), and the National High-Tech R&D Program of China (2011AA100102).

Association analysis of grain traits with SSR markers between Aegilops tauschii and hexaploid wheat (Triticum aestivum L.)

ZHAO Jing-lan, WANG Hong-wei, ZHANG Xiao-cun, DU Xu-ye, LI An-fei, KONG Ling-rang

1、State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, P.R.China
2、Taishan Polytechnic, Tai’an 271000, P.R.China

Received:2014-10-24 Online:2015-10-09 Published:2015-10-12
Contact: KONG Ling-rang, Tel: +86-538-8249278,E-mail: lkong@sdau.edu.cn
About author:ZHAO Jing-lan, Tel: +86-538-8628134, E-mail: zhaojlan@sina. com;
Supported by:
We acknowledge financial supports by the National 973 Program of China (2014CB138100), the National Natural Science Foundation of China (31171553, 31471488 and 31200982), and the National High-Tech R&D Program of China (2011AA100102).

摘要/Abstract

摘要： Seven important grain traits, including grain length (GL), grain width (GW), grain perimeter (GP), grain area (GA), grain length/width ratio (GLW), roundness (GR), and thousand-grain weight (TGW), were analyzed using a set of 139 simple sequence repeat (SSR) markers in 130 hexaploid wheat varieties and 193 Aegilops tauschii accessions worldwide. In total, 1 612 alleles in Ae. tauschii and 1 360 alleles in hexaploid wheat (Triticum aestivum L.) were detected throughout the D genome. 197 marker-trait associations in Ae. tauschii were identified with 58 different SSR loci in 3 environments, and the average phenotypic variation value (R2) ranged from 0.68 to 15.12%. In contrast, 208 marker-trait associations were identified in wheat with 66 different SSR markers in 4 environments and the average phenotypic R2 ranged from 0.90 to 19.92%. Further analysis indicated that there are 6 common SSR loci present in both Ae. tauschii and hexaploid wheat, which are significantly associated with the 5 investigated grain traits (i.e., GA, GP, GR, GL, and TGW) and in total, 16 alleles derived from the 6 aforementioned SSR loci were shared by Ae. tauschii and hexaploid wheat. These preliminary data suggest the existence of common alleles may explain the evolutionary process and the selection between Ae. tauschii and hexaploid wheat. Furthermore, the genetic differentiation of grain shape and thousand-grain weight were observed in the evolutionary developmental process from Ae. tauschii to hexaploid wheat.

关键词: association analysis , grain traits , Aegilops tauschii , Triticum aestivum , SSR markers

Abstract: Seven important grain traits, including grain length (GL), grain width (GW), grain perimeter (GP), grain area (GA), grain length/width ratio (GLW), roundness (GR), and thousand-grain weight (TGW), were analyzed using a set of 139 simple sequence repeat (SSR) markers in 130 hexaploid wheat varieties and 193 Aegilops tauschii accessions worldwide. In total, 1 612 alleles in Ae. tauschii and 1 360 alleles in hexaploid wheat (Triticum aestivum L.) were detected throughout the D genome. 197 marker-trait associations in Ae. tauschii were identified with 58 different SSR loci in 3 environments, and the average phenotypic variation value (R2) ranged from 0.68 to 15.12%. In contrast, 208 marker-trait associations were identified in wheat with 66 different SSR markers in 4 environments and the average phenotypic R2 ranged from 0.90 to 19.92%. Further analysis indicated that there are 6 common SSR loci present in both Ae. tauschii and hexaploid wheat, which are significantly associated with the 5 investigated grain traits (i.e., GA, GP, GR, GL, and TGW) and in total, 16 alleles derived from the 6 aforementioned SSR loci were shared by Ae. tauschii and hexaploid wheat. These preliminary data suggest the existence of common alleles may explain the evolutionary process and the selection between Ae. tauschii and hexaploid wheat. Furthermore, the genetic differentiation of grain shape and thousand-grain weight were observed in the evolutionary developmental process from Ae. tauschii to hexaploid wheat.

Key words: association analysis , grain traits , Aegilops tauschii , Triticum aestivum , SSR markers

ZHAO Jing-lan, WANG Hong-wei, ZHANG Xiao-cun, DU Xu-ye, LI An-fei, KONG Ling-rang. Association analysis of grain traits with SSR markers between Aegilops tauschii and hexaploid wheat (Triticum aestivum L.)[J]. Journal of Integrative Agriculture, 2015, 14(10): 1936-1948.

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Association analysis of grain traits with SSR markers between Aegilops tauschii and hexaploid wheat (Triticum aestivum L.)

Association analysis of grain traits with SSR markers between Aegilops tauschii and hexaploid wheat (Triticum aestivum L.)

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