GeneticStructureandDiversityofParentalCultivars Involved inChinaMainland Sugarcane Breeding Programs as Inferred from DNA Microsatellites

doi:10.1016/S1671-2927(00)8714

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (11): 1794-1803.DOI: 10.1016/S1671-2927(00)8714

GeneticStructureandDiversityofParentalCultivars Involved inChinaMainland Sugarcane Breeding Programs as Inferred from DNA Microsatellites

QI Yong-wen, PANYong-bao, LAO Fang-ye, ZHANG Chui-ming, FAN Li-na, HE Hui-yi, LIU Rui, WANG Qin-nan, LIU Shao-mou, LIU Fu-ye, LI Qi-wei , DENG Hai-hua

1.Guangdong Key Laboratory of Sugarcane Improvement and Biorefinery, Guangzhou Sugarcane Industry Research Institute, Guangzhou
510316, P.R.China
2.USDA-ARS, Mid-South Area, Sugarcane Research Laboratory, Houma 70360, USA

收稿日期:2011-03-04 出版日期:2012-11-01 发布日期:2012-11-24
通讯作者: Correspondence DENG Hai-hua, Tel: +86-20-84178327, E-mail: haihuadeng@126.com
作者简介:QI Yong-wen, Tel: +86-20-84168434, E-mail: yongwen2001@163.com
基金资助:
This work was supported by the National Natural Science Foundation of China (30800700), and the China Agriculture Research System.

GeneticStructureandDiversityofParentalCultivars Involved inChinaMainland Sugarcane Breeding Programs as Inferred from DNA Microsatellites

QI Yong-wen, PANYong-bao, LAO Fang-ye, ZHANG Chui-ming, FAN Li-na, HE Hui-yi, LIU Rui, WANG Qin-nan, LIU Shao-mou, LIU Fu-ye, LI Qi-wei , DENG Hai-hua

1.Guangdong Key Laboratory of Sugarcane Improvement and Biorefinery, Guangzhou Sugarcane Industry Research Institute, Guangzhou
510316, P.R.China
2.USDA-ARS, Mid-South Area, Sugarcane Research Laboratory, Houma 70360, USA

Received:2011-03-04 Online:2012-11-01 Published:2012-11-24
Contact: Correspondence DENG Hai-hua, Tel: +86-20-84178327, E-mail: haihuadeng@126.com
About author:QI Yong-wen, Tel: +86-20-84168434, E-mail: yongwen2001@163.com
Supported by:
This work was supported by the National Natural Science Foundation of China (30800700), and the China Agriculture Research System.

摘要/Abstract

摘要： To understand genetic structure and diversity of parental cultivars involved in China Mainland sugarcane breeding programs, 92 elite parents and 4 wild relatives were genotyped with 18 microsatellite DNA markers. The genetic similarity (GS) values among the cultivars ranged from 0.346 to 0.960 with an average of 0.533. Among the introduced cultivars, India accessions had the closest genetic distance to China Mainland accessions (0.447), while Australia accessions have the furthest distance (0.503). A comparison of allelic diversity among geographical origins showed that there were 22 China Mainland specific alleles, of which 28% were derived from native S. spontaneaum germplasm in China. Model-based genetic structure, clustering, and principal components analyses consistently revealed there were five groups within the 96 accessions. Groups 1, 2, 4, and 5 consisted of all cultivars and group 3 only contained wild germplasm. Group 2 was characterized as the Introduction group with 46 cultivars predominantly introduced from Australia, Taiwan of China, India, and USA. Groups 1, 4, and 5 consisted of cultivars mostly originated from China Mainland, defined as the Complex group, Yacheng lines group, and F134/CP72-1210 group, respectively, upon their pedigree. By understanding the genetic relationships among the parental cultivars, breeders can gain a rational basis for expanding the gene pool and select the best parental accessions for crossing.

关键词: genetic diversity, microsatellite DNA marker, population structure, sugarcane

Abstract: To understand genetic structure and diversity of parental cultivars involved in China Mainland sugarcane breeding programs, 92 elite parents and 4 wild relatives were genotyped with 18 microsatellite DNA markers. The genetic similarity (GS) values among the cultivars ranged from 0.346 to 0.960 with an average of 0.533. Among the introduced cultivars, India accessions had the closest genetic distance to China Mainland accessions (0.447), while Australia accessions have the furthest distance (0.503). A comparison of allelic diversity among geographical origins showed that there were 22 China Mainland specific alleles, of which 28% were derived from native S. spontaneaum germplasm in China. Model-based genetic structure, clustering, and principal components analyses consistently revealed there were five groups within the 96 accessions. Groups 1, 2, 4, and 5 consisted of all cultivars and group 3 only contained wild germplasm. Group 2 was characterized as the Introduction group with 46 cultivars predominantly introduced from Australia, Taiwan of China, India, and USA. Groups 1, 4, and 5 consisted of cultivars mostly originated from China Mainland, defined as the Complex group, Yacheng lines group, and F134/CP72-1210 group, respectively, upon their pedigree. By understanding the genetic relationships among the parental cultivars, breeders can gain a rational basis for expanding the gene pool and select the best parental accessions for crossing.

Key words: genetic diversity, microsatellite DNA marker, population structure, sugarcane

QI Yong-wen, PANYong-bao, LAO Fang-ye, ZHANG Chui-ming, FAN Li-na, HE Hui-yi, LIU Rui, WANG Qin-nan, LIU Shao-mou, LIU Fu-ye, LI Qi-wei , DENG Hai-hua. GeneticStructureandDiversityofParentalCultivars Involved inChinaMainland Sugarcane Breeding Programs as Inferred from DNA Microsatellites[J]. Journal of Integrative Agriculture, 2012, 12(11): 1794-1803.

QI Yong-wen, PANYong-bao , LAO Fang-ye, ZHANG Chui-ming, FAN Li-na, HE Hui-yi, LIU Rui, WANG Qin-nan, LIU Shao-mou, LIU Fu-ye, LI Qi-wei , DENG Hai-hua. GeneticStructureandDiversityofParentalCultivars Involved inChinaMainland Sugarcane Breeding Programs as Inferred from DNA Microsatellites[J]. Journal of Integrative Agriculture, 2012, 12(11): 1794-1803.

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GeneticStructureandDiversityofParentalCultivars Involved inChinaMainland Sugarcane Breeding Programs as Inferred from DNA Microsatellites

GeneticStructureandDiversityofParentalCultivars Involved inChinaMainland Sugarcane Breeding Programs as Inferred from DNA Microsatellites

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