Genetics and Molecular Mapping of a High-Temperature Resistance Gene to Stripe Rust in Seeding-Stage in Winter Wheat Cultivar Lantian

doi:10.1016/S2095-3119(13)60322-6

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (6): 1018-1025.DOI: 10.1016/S2095-3119(13)60322-6

Genetics and Molecular Mapping of a High-Temperature Resistance Gene to Stripe Rust in Seeding-Stage in Winter Wheat Cultivar Lantian

MA Dong-fang, JING Jin-xue, HOU Dong-yuan, LI Qiang, ZHOU Xin-li, DU Jiu-yuan , LU Qing-lin

1 State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling 712100,P.R.China
2 Wheat Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, P.R.China

收稿日期:2012-07-27 出版日期:2013-06-01 发布日期:2013-06-09
通讯作者: Correspondence JING Jin-xue, Tel: +86-29-87092434, E-mail: jingjinxue@163.com
作者简介:MA Dong-fang, E-mail: madongfang1984@163.com
基金资助:
The financial support of the 111 Project from the Ministry of Education of China (B07049), the Key Technologies R&D Program of China during the 11th Five-Year Plan period (2006BAD08A05) and the project of Toxicity Variation of Wheat Stripe Rust Pathogen and Demonstration of Integrated Management of Stripe Rust, China (200903035-02) are thankfully acknowledged.

Genetics and Molecular Mapping of a High-Temperature Resistance Gene to Stripe Rust in Seeding-Stage in Winter Wheat Cultivar Lantian

MA Dong-fang, JING Jin-xue, HOU Dong-yuan, LI Qiang, ZHOU Xin-li, DU Jiu-yuan , LU Qing-lin

1 State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling 712100,P.R.China
2 Wheat Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, P.R.China

Received:2012-07-27 Online:2013-06-01 Published:2013-06-09
Contact: Correspondence JING Jin-xue, Tel: +86-29-87092434, E-mail: jingjinxue@163.com
About author:MA Dong-fang, E-mail: madongfang1984@163.com
Supported by:
The financial support of the 111 Project from the Ministry of Education of China (B07049), the Key Technologies R&D Program of China during the 11th Five-Year Plan period (2006BAD08A05) and the project of Toxicity Variation of Wheat Stripe Rust Pathogen and Demonstration of Integrated Management of Stripe Rust, China (200903035-02) are thankfully acknowledged.

摘要/Abstract

摘要： Stripe rust, caused by Puccinia striiformis Westend. f. sp. tritici (Pst), is a severe foliar disease of common wheat (Triticum aestivum L.) in the world. Resistance is the best approach to control the disease. The winter wheat cultivar Lantian 1 has high-temperature resistance to stripe rust. To determing the gene(s) for the stripe rust resistance, Lantian 1 was crossed with Mingxian 169 (M169). Seedlings of the parents, and F1, F2 and F2-3 progenies were tested with races CYR32 of Pst under controlled greenhouse conditions. Lantian 1 has a single partially dominant gene conferred resistance to race CYR32, designated as YrLT1. Simple sequence repeat (SSR) techniques were used to identify molecular markers linked to YrLT1. A linkage group of five SSR markers was constructed for YrLT1 using 166 F2 plants. Based on the SSR marker consensus map and the position on wheat chromosome, the resistance gene was assigned on chromosome 2DL. Amplification of a set of nulli-tetrasomic Chinese Spring lines with SSR marker Xwmc797 confirmed that the resistance gene was located on the long arm of chromosome 2D. Because of its chromosomal location and the high-temperature resistance, this gene is different from previously described genes. The molecular map spanned 29.9 cM, and the genetic distance of two close markers Xbarc228 and Xcfd16 to resistance gene locus was 4.0 and 5.7 cM, respectively. The polymorphism rates of the flanking markers in 46 wheat lines were 2.1 and 2.1%, respectively; and the two markers in combination could distinguish the alleles at the resistance locus in 97.9% of tested genotypes. This new gene and flanking markers should be useful in developing wheat cultivars with high level and possible durable resistance to stripe rust.

关键词: Puccinia striiformis f. sp. tritici , high-temperature resistance , genetic analysis , molecular mapping

Abstract: Stripe rust, caused by Puccinia striiformis Westend. f. sp. tritici (Pst), is a severe foliar disease of common wheat (Triticum aestivum L.) in the world. Resistance is the best approach to control the disease. The winter wheat cultivar Lantian 1 has high-temperature resistance to stripe rust. To determing the gene(s) for the stripe rust resistance, Lantian 1 was crossed with Mingxian 169 (M169). Seedlings of the parents, and F1, F2 and F2-3 progenies were tested with races CYR32 of Pst under controlled greenhouse conditions. Lantian 1 has a single partially dominant gene conferred resistance to race CYR32, designated as YrLT1. Simple sequence repeat (SSR) techniques were used to identify molecular markers linked to YrLT1. A linkage group of five SSR markers was constructed for YrLT1 using 166 F2 plants. Based on the SSR marker consensus map and the position on wheat chromosome, the resistance gene was assigned on chromosome 2DL. Amplification of a set of nulli-tetrasomic Chinese Spring lines with SSR marker Xwmc797 confirmed that the resistance gene was located on the long arm of chromosome 2D. Because of its chromosomal location and the high-temperature resistance, this gene is different from previously described genes. The molecular map spanned 29.9 cM, and the genetic distance of two close markers Xbarc228 and Xcfd16 to resistance gene locus was 4.0 and 5.7 cM, respectively. The polymorphism rates of the flanking markers in 46 wheat lines were 2.1 and 2.1%, respectively; and the two markers in combination could distinguish the alleles at the resistance locus in 97.9% of tested genotypes. This new gene and flanking markers should be useful in developing wheat cultivars with high level and possible durable resistance to stripe rust.

Key words: Puccinia striiformis f. sp. tritici , high-temperature resistance , genetic analysis , molecular mapping

MA Dong-fang, JING Jin-xue, HOU Dong-yuan, LI Qiang, ZHOU Xin-li, DU Jiu-yuan , LU Qing-lin. Genetics and Molecular Mapping of a High-Temperature Resistance Gene to Stripe Rust in Seeding-Stage in Winter Wheat Cultivar Lantian[J]. Journal of Integrative Agriculture, 2013, 12(6): 1018-1025.

参考文献

[1]Chen X M. 2005. Epidemiology and control of stripe rust(Puccinia striiformis f. sp. tritici) on wheat. CanadianJournal of Plant Pathology, 27, 314-337

[2]Chen X M, Line R F. 1995. Gene number and inheritabilityof wheat cultivars with durable, high-temperature, adultplantresistance and race-specific resistance toPuccinia striiformis. Phytopathology, 85, 573-578

[3]Chen X M, Penman L, Wan A M, Cheng P. 2010. Virulenceraces of Puccinia striiformis f. sp. tritici in 2006 and2007 and development of wheat stripe rust anddistributions, dynamics, and evolutionary relationships of races from 2000 to 2007 in the United States.Canadian Journal of Plant Pathology, 32, 315-333

[4]Chen X, Line R F, Jones S S. 1995. Chromosomal locationof genes for resistance to Puccinia striiformis in winterwheat cultivars Heines VII, Clement, Moro, Tyee, Tres,and Daws. Phytopathology, 85, 1362-1367

[5]Hou L, Ma D F, Hu M L, Lu Y, Jing J X. 2013. Geneticanalysis and molecular mapping of a all-stage striperust resistance gene in Triticum aestivum-Hayaldiavillosa translocation line V3. Journal of IntegrativeAgriculture, doi:10.1016/S2095-3119(13)60293-2Jagger L J, Newell C, Berry S T, MacCormack R, Boyd L A

[6]2011. The genetic characterisation of stripe rustresistance in the German wheat cultivar Alcedo.Theoretical and Applied Genetics, 122, 723-733

[7]Kosambi D. 1943. The estimation of map distances fromrecombination values. Annals of Human Genetics, 12,172-175

[8]Line R F. 2002. Stripe rust of wheat and barley in NorthAmerica: a retrospective historical review. AnnualReview of Phytopathology, 40, 75-118

[9]Ma D F, Hou L, Tang M S, Wang H G, Li Q, Jing J X. 2013.Genetic analysis and molecular mapping of a stripe rustresistance gene YrH9014 in wheat line H9014-14-4-6-1

[10]Journal of Integrative Agriculture, 12, 638-645

[11]Lincoln S, Daly M E L. 1992. Constructing genetic mapswith Mapmarker/EXP3.0. In: Whitehead InstituteTechnical Report. 3rd ed. Whitehead Institute,Cambridge.Liu R, Meng J. 2003. MapDraw: a microsoft excel macro fordrawing genetic linkage maps based on given geneticlinkage data. Hereditas, 25, 317.Marais G F, McCallum B, Snyman J E. 2005. Leaf rust andstripe rust resistance genes Lr54 and Yr37 transferredto wheat from Aegilops kotschyi. Plant Breeding, 124,538-541

[12]McIntosh R A, Dubcovsky J, Rogers W J, Morris C, AppelsR, Xia X C. 2011. Catalogue of gene symbols for wheat:2011 supplement. Annual Wheat Newsletter, 56, 273-282

[13]Melichar J P E, Berry S, Newell C, MacCormack R, Boyd L A.2008. QTL identification and microphenotypecharacterization of the developmentally regulated yellowrust resistance in the UK wheat cultivar Guardian.Theoretical and Applied Genetics, 117, 391-399

[14]Ren Q, Liu H J, Zhang Z Y, Feng J, Xu S C, Pu Z J, Xin Z Y.2012. Characterization and molecular mapping of a striperust resistance gene in synthetic wheat CI110. Journalof Integrative Agriculture, 11, 521-527

[15]Qayoum A, Line R F. 1985. High-temperature, adult-plantresistance to stripe rust of wheat. Phytopathology, 75,1121-1125

[16]Ren Y, He Z H, Li J, Lillemo M, Wu L, Bai B, Lu Q X, Zhu HZ, Zhou G, Du J Y, et al. 2012. QTL mapping of adultplantresistance to stripe rust in a population derivedfrom common wheat cultivars Naxos and Shanghai 3/Catbird. Theoretical and Applied Genetics, 125, 1211-1221

[17]Ren Y, He Z H, Li J, Lillemo M, Wu L, Bai B, Lu Q X, Zhu HZ, Zhou G, Du J Y, et al. 2012. QTL mapping of adultplantresistances to stripe rust and leaf rust in Chinesewheat cultivar Bainong 64. Theoretical and AppliedGenetics, 125, 1253-1262

[18]Rogers S O, Bendich A J. 1985. Extraction of DNA frommilligram amounts of fresh, herbarium and mummifiedplant tissues. Plant Molecular Biology, 5, 69-76

[19]Röder M S, Korzun V, Wendehake K, Plaschke J.1998. Amicrosatellite map of wheat. Genetics, 149, 2007-2023

[20]Somers D J, Isaac P, Edwards K. 2004. A high-densitymicrosatellite consensus map for bread wheat (Triticumaestivum L.). Theoretical and Applied Genetics, 109,1105-1114

[21]Stubbs R W. 1985. Stripe rust. In: Roelfs A P, Bushnell WR, eds., The Cereal Rusts II. Academic Press, Orlando,FL. pp. 61-101

[22]Suenaga K, Singh R P, Huerta-Espino J, William H M. 2003.Microsatellite markers for genes Lr34/Yr18 and otherquantitative trait loci for leaf rust and stripe rustresistance in bread wheat. Phytopathology, 93, 881-890

[23]Tixier M H, Sourdille P S. 1997. Detection of wheatmicrosallite using no-radioactive silvermitrate stainingmethod. Genetic Breed, 51, 175-177

[24]Wan A M, Wu L R. 2003. Pathogenic changes of stripe rustfungus of wheat in China during 1997-1999

[25]ActaPhytopathologica Sinica, 33, 261-266 (in Chinese)

[26]Wan A M, Zhao Z H, Chen X M, He Z H, Jin S L, Jia Q Z,Yao G, Yang J X, Wang B T, Li G B, et al. 2004. Wheatstripe rust epidemic and virulence of Puccinia striiformisf. sp. tritici in China in 2002. Plant Disease, 88, 896-904

[27]Wang F L, Wu L R, Wan A M, Song W Z, Yuan W H, YangJ X. 1994. Postulated genes for resistance to stripe rustin seedlings of wheat cultivars from Shannxi, Gansuand Sichuan Provinces. Acta Agronomica Sinica, 2,589-594 (in Chinese)

[28]Wellings C R. 2011. Global status of stripe rust: a review ofhistorical and current threats. Euphytica, 179, 129-141

[29]Weng D X, Xu S C, Lin R M, Wan A M, Li J P, Wu L R. 2005.Microsatellite marker linked with stripe rust resistantgene Yr9 in wheat. Acta Genetica Sinica, 32, 937-941(in Chinese)

[30]Worland A J, Law C N. 1986. Genetic analysis ofchromosome 2D of wheat I. The location of genesaffecting height, day-length insensitivity, hybriddwarfism and yellow-rust resistance. Zeitschrift furPflanzenzuchtung, 96, 331-345

[31]Yang Z M , Xie C J, Sun Q X. 2003. Situation of the sourcesof stripe rust resistance of wheat in the Post-CY32 erain China. Acta Agronomica Sinica, 29, 161-168 (in Chinese)

Genetics and Molecular Mapping of a High-Temperature Resistance Gene to Stripe Rust in Seeding-Stage in Winter Wheat Cultivar Lantian

Genetics and Molecular Mapping of a High-Temperature Resistance Gene to Stripe Rust in Seeding-Stage in Winter Wheat Cultivar Lantian

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	QIU Cai-ling, ZHANG Zhi-xiang, LI Shi-fang, BAI Yan-ju, LIU Shang-wu, FAN Guo-quan, GAO Yan-ling, ZHANG Wei, ZHANG Shu, Lü Wen-he, Lü Dian-qiu. Occurrence and molecular characterization of Potato spindle tuber viroid (PSTVd) isolates from potato plants in North China[J]. Journal of Integrative Agriculture, 2016, 15(2): 349-363.
[2]	HAN Liu-sha, LI Zai-feng, WANG Jia-zhen, SHI Ling-zhi, ZHU Lin, LI Xing, LIU Da-qun, Syed J A Shah. Molecular mapping of leaf rust resistance genes in the wheat line Yu 356-9[J]. Journal of Integrative Agriculture, 2015, 14(7): 1223-1228.
[3]	SONG Zhen, LI Zhong-an, LIU Ke-hong, ZHOU Chang-yong. Complete genome sequence analysis of two Citrus tatter leaf virus (CTLV) isolates from China[J]. Journal of Integrative Agriculture, 2015, 14(5): 984-987.
[4]	LIU Ze-guang, YAO Wei-yuan, SHEN Xue-xue, CHAO Kai-xiang, FAN Yu, LI Min-zhou, WANG Baotong, LI Qiang , JING Jin-xue. Molecular Mapping of a Stripe Rust Resistance Gene YrH9020a Transferred from Psathyrostachys huashanica Keng on Wheat Chromosome 6D[J]. Journal of Integrative Agriculture, 2014, 13(12): 2577-2583.
[5]	YI Long, ZHOU Chang-yong. Phylogenetic Analysis of Citrus tristeza virus Isolates of Wild Type Citrus in China[J]. Journal of Integrative Agriculture, 2014, 13(12): 2669-2677.
[6]	XU Liang, JIANG Qiu-wei, WU Jian, WANG Yan, GONG Yi-qin, WANG Xian-li, Limera Cecilia　, LIU Li-wang. Identification and Molecular Mapping of the RsDmR Locus Conferring Resistance to Downy Mildew at Seedling Stage in Radish (Raphanus sativus L.)[J]. Journal of Integrative Agriculture, 2014, 13(11): 2362-2369.
[7]	MA Dong-fang, HOU Lu, TANG Ming-shuang, WANG Hai-ge, LI Qiang , JING Jin-xue. Genetic Analysis and Molecular Mapping of a Stripe Rust Resistance Gene YrH9014 in Wheat Line H9014-14-4-6-1[J]. Journal of Integrative Agriculture, 2013, 12(4): 638-645.
[8]	HOU Lu, MA Dong-fang, HU Mao-lin, HE Miao-miao, LU Yan , JING Jin-xue. Genetic Analysis and Molecular Mapping of an All-Stage Stripe Rust Resistance Gene in Triticum aestivum-Haynaldia villosa Translocation Line V3[J]. Journal of Integrative Agriculture, 2013, 12(12): 2197-2208.
[9]	LIAN Wei-wei, TANG Yi-miao, GAO Shi-qing, ZHANG Zhao, ZHAO Xin, ZHAO Chang-ping. Phylogenetic Analysis and Expression Patterns of the MAPK Gene Family in Wheat (Triticum aestivum L.)[J]. Journal of Integrative Agriculture, 2012, 12(8): 1227-1235.
[10]	REN Qiang, LIU Hui-juan, ZHANG Zeng-yan, FENG Jing, XU Shi-chang, PU Zong-jun , XIN Zhiyong. Characterization and Molecular Mapping of a Stripe Rust Resistance Gene in Synthetic Wheat CI110[J]. Journal of Integrative Agriculture, 2012, 12(4): 521-·527.
[11]	Paul J De Barro. The Bemisia tabaci Species Complex: Questions to Guide Future Research[J]. Journal of Integrative Agriculture, 2012, 11(2): 187-196.
[12]	DUAN Yuan-lin, GUAN Hua-zhong, ZHUO Ming, CHEN Zhi-wei, LI Wen-tao, PAN Run-sen, MAO Da-mei, ZHOU Yuan-chang, WU Wei-ren. Genetic Analysis and Mapping of an Enclosed Panicle Mutant Locus esp1 in Rice (Oryza sativa L.)[J]. Journal of Integrative Agriculture, 2012, 12(12): 1933-1939.
[13]	CAO Jun, SHI Feng. Genomic Analysis of Mitochondrial Carrier Genes in the Bombyx mori[J]. Journal of Integrative Agriculture, 2012, 12(10): 1707-1713.
[14]	GU Shao-peng, SHI Xin-tao, SHI Zhong-yong, WANG Zhong-bing, ZHENG Ming-xue. Identification and Phylogenetic Analysis of an Orf Virus Isolated from an Outbreak in Boer Goat in Shanxi Province[J]. Journal of Integrative Agriculture, 2011, 10(6): 946-953.
[15]	WU Xiao-ling, ZHANG Bao-qiang, SUN Shi, ZHAO Jin-ming, YANG Feng, GUO Na, GAI Jun-yi, XING Han. Identification, Genetic Analysis and Mapping of Resistance to Phytophthora sojae of Pm28 in Soybean[J]. Journal of Integrative Agriculture, 2011, 10(10): 1506-1511.