Genetic Analysis and Molecular Mapping of a Stripe Rust Resistance Gene YrH9014 in Wheat Line H9014-14-4-6-1

doi:10.1016/S2095-3119(13)60271-3

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (4): 638-645.DOI: 10.1016/S2095-3119(13)60271-3

Genetic Analysis and Molecular Mapping of a Stripe Rust Resistance Gene YrH9014 in Wheat Line H9014-14-4-6-1

MA Dong-fang, HOU Lu, TANG Ming-shuang, WANG Hai-ge, LI Qiang , JING Jin-xue

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling 712100, P.R.China

收稿日期:2012-07-20 出版日期:2013-04-01 发布日期:2013-04-07
通讯作者: Correspondence JING Jin-xue, Tel: +86-29-87092434, E-mail:jingjinxue@163.com
作者简介:MA Dong-fang, E-mail: madongfang1984@163.com; HOU Lu, E-mail: mantou428@163.com
基金资助:
The research was supported by the 111 Project from the Education Ministry of China (B07049) and the National 11th Five-Year Plan Key Project (2006BAD08A05) and Toxicity Variation of Wheat Stripe Rust Pathogen and Demonstration of Integrated Management of Stripe Rust, China (200903035-02).

Genetic Analysis and Molecular Mapping of a Stripe Rust Resistance Gene YrH9014 in Wheat Line H9014-14-4-6-1

MA Dong-fang, HOU Lu, TANG Ming-shuang, WANG Hai-ge, LI Qiang , JING Jin-xue

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling 712100, P.R.China

Received:2012-07-20 Online:2013-04-01 Published:2013-04-07
Contact: Correspondence JING Jin-xue, Tel: +86-29-87092434, E-mail:jingjinxue@163.com
About author:MA Dong-fang, E-mail: madongfang1984@163.com; HOU Lu, E-mail: mantou428@163.com
Supported by:
The research was supported by the 111 Project from the Education Ministry of China (B07049) and the National 11th Five-Year Plan Key Project (2006BAD08A05) and Toxicity Variation of Wheat Stripe Rust Pathogen and Demonstration of Integrated Management of Stripe Rust, China (200903035-02).

摘要/Abstract

摘要： Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most widespread and destructive wheat diseases in many wheat-growing regions of the world. The winter wheat translocation line H9014-14-4-6-1 has all stage resistance. To identify stripe rust resistance genes, the segregating populations were developed from the cross between H9014-14-4-6-1 and Mingxian 169 (a wheat cultivar susceptible to all Pst races identified in China). The seedlings of the parents and F1 plants, F2, F3 and BC1 generations were tested with Pst races under controlled greenhouse conditions. Two genes for resistance to stripe rust were identified, one dominant gene conferred resistance to SUN11-4, temporarily designated YrH9014 and the other recessive gene conferred resistance to CYR33. The bulked segregant analysis and simple sequence repeat (SSR) markers were used to identify polymorphic markers associated with YrH9014. Seven polymorphic SSR markers were used to genotype the F2 population inoculated with SUN11-4. A linkage map was constructed according to the genotypes of seven SSR markers and resistance gene. The molecular map spanned 24.3 cM, and the genetic distance of the two closest markers Xbarc13 and Xbarc55 to gene locus was 1.4 and 3.6 cM, respectively. Based on the position of SSR marker, the resistance gene YrH9014 was located on chromosome arm 2BS. Amplification of a set of nulli-tetrasomic Chinese Spring lines with SSR marker Xbarc13 indicated that YrH9014 was located on chromosome 2B. Based on chromosomal location, the reaction patterns and pedigree analysis, YrH9014 should be a novel resistance gene to stripe rust. This new gene and flanking markers got from this study should be useful for marker-assisted selection (MAS) in breeding programs for stripe rust.

关键词: wheat cultivar , Puccinia striiformis f. sp. tritici , resistance genes , molecular mapping , Psathyrostachys huashanica Keng

Abstract: Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most widespread and destructive wheat diseases in many wheat-growing regions of the world. The winter wheat translocation line H9014-14-4-6-1 has all stage resistance. To identify stripe rust resistance genes, the segregating populations were developed from the cross between H9014-14-4-6-1 and Mingxian 169 (a wheat cultivar susceptible to all Pst races identified in China). The seedlings of the parents and F1 plants, F2, F3 and BC1 generations were tested with Pst races under controlled greenhouse conditions. Two genes for resistance to stripe rust were identified, one dominant gene conferred resistance to SUN11-4, temporarily designated YrH9014 and the other recessive gene conferred resistance to CYR33. The bulked segregant analysis and simple sequence repeat (SSR) markers were used to identify polymorphic markers associated with YrH9014. Seven polymorphic SSR markers were used to genotype the F2 population inoculated with SUN11-4. A linkage map was constructed according to the genotypes of seven SSR markers and resistance gene. The molecular map spanned 24.3 cM, and the genetic distance of the two closest markers Xbarc13 and Xbarc55 to gene locus was 1.4 and 3.6 cM, respectively. Based on the position of SSR marker, the resistance gene YrH9014 was located on chromosome arm 2BS. Amplification of a set of nulli-tetrasomic Chinese Spring lines with SSR marker Xbarc13 indicated that YrH9014 was located on chromosome 2B. Based on chromosomal location, the reaction patterns and pedigree analysis, YrH9014 should be a novel resistance gene to stripe rust. This new gene and flanking markers got from this study should be useful for marker-assisted selection (MAS) in breeding programs for stripe rust.

Key words: wheat cultivar , Puccinia striiformis f. sp. tritici , resistance genes , molecular mapping , Psathyrostachys huashanica Keng

MA Dong-fang, HOU Lu, TANG Ming-shuang, WANG Hai-ge, LI Qiang , JING Jin-xue. Genetic Analysis and Molecular Mapping of a Stripe Rust Resistance Gene YrH9014 in Wheat Line H9014-14-4-6-1[J]. Journal of Integrative Agriculture, 2013, 12(4): 638-645.

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Genetic Analysis and Molecular Mapping of a Stripe Rust Resistance Gene YrH9014 in Wheat Line H9014-14-4-6-1

Genetic Analysis and Molecular Mapping of a Stripe Rust Resistance Gene YrH9014 in Wheat Line H9014-14-4-6-1

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