Identification and Molecular Mapping of the RsDmR Locus Conferring Resistance to Downy Mildew at Seedling Stage in Radish (Raphanus sativus L.)

doi:10.1016/S2095-3119(14)60792-9

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (11): 2362-2369.DOI: 10.1016/S2095-3119(14)60792-9

Identification and Molecular Mapping of the RsDmR Locus Conferring Resistance to Downy Mildew at Seedling Stage in Radish (Raphanus sativus L.)

XU Liang, JIANG Qiu-wei, WU Jian, WANG Yan, GONG Yi-qin, WANG Xian-li, Limera Cecilia　, LIU Li-wang

1、National Key Laboratory of Crop Genetics and Germplasm Enhancement/Engineering Research Center of Horticultural Crop Germplasm
Enhancement and Utilization, Ministry of Education/College of Horticulture, Nanjing Agricultural University, Nanjing 210095, P.R.China
2、Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

收稿日期:2013-11-11 出版日期:2014-11-06 发布日期:2014-11-14
通讯作者: LIU Li-wang, Tel: +86-25-84395563, Fax: +86-25-84395266, E-mail: nauliulw@njau.edu.cn
作者简介:XU Liang, E-mail: nauxuliang@njau.edu.cn,* These authors contributed equally to this study.
基金资助:
This work was in part supported by grants from the National Key Technologies R&D Program of China (2012BAD02B01), the Key Technology R&D Program of Jiangsu Province, China (BE2013429), Jiangsu Agricultural Science and Technology Innovation Fund (JASTIF,CX(12) 2006, (13)2007], NAUNKL- ZW2009007 and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

Identification and Molecular Mapping of the RsDmR Locus Conferring Resistance to Downy Mildew at Seedling Stage in Radish (Raphanus sativus L.)

XU Liang, JIANG Qiu-wei, WU Jian, WANG Yan, GONG Yi-qin, WANG Xian-li, Limera Cecilia　, LIU Li-wang

1、National Key Laboratory of Crop Genetics and Germplasm Enhancement/Engineering Research Center of Horticultural Crop Germplasm
Enhancement and Utilization, Ministry of Education/College of Horticulture, Nanjing Agricultural University, Nanjing 210095, P.R.China
2、Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

Received:2013-11-11 Online:2014-11-06 Published:2014-11-14
Contact: LIU Li-wang, Tel: +86-25-84395563, Fax: +86-25-84395266, E-mail: nauliulw@njau.edu.cn
About author:XU Liang, E-mail: nauxuliang@njau.edu.cn,* These authors contributed equally to this study.
Supported by:
This work was in part supported by grants from the National Key Technologies R&D Program of China (2012BAD02B01), the Key Technology R&D Program of Jiangsu Province, China (BE2013429), Jiangsu Agricultural Science and Technology Innovation Fund (JASTIF,CX(12) 2006, (13)2007], NAUNKL- ZW2009007 and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

摘要/Abstract

摘要： Downy mildew (DM), caused by the fungus Peronospora parasitica, is a destructive disease of radish (Raphanus sativus L.) worldwide. Host resistance has been considered as an attractive and environmentally friendly approach to control the disease. However, the genetic mechanisms of resistance in radish to the pathogen remain unknown. To determine the inheritance of resistance to DM, F1, F2 and BC1F1 populations derived from reciprocal crosses between a resistant line NAU-dhp08 and a susceptible line NAU-qtbjq-06 were evaluated for their responses to DM at seedling stage. All F1 hybrid plants showed high resistance to DM and maternal effect was not detected. The segregation for resistant to susceptible individuals statistically fitted a 3:1 ratio in two F2 populations (F2(SR) and F2(RS)), and 1:1 ratio in two BC1F1 populations, indicating that resistance to DM at seedling stage in radish was controlled by a single dominant locus designated as RsDmR. A total of 1 972 primer pairs (1036 SRAP, 628 RAPD, 126 RGA, 110 EST-SSR and 72 ISSR) were screened, and 36 were polymorphic between the resistant and susceptible bulks, and consequently used for genotyping individuals in the F2 population. Three markers (Em9/ga24370, NAUISSR826700 and Me7/em10400) linked to the RsDmR locus within a 10.0 cM distance were identified using bulked segregant analysis (BSA). The SRAP marker Em9/ga24370 was the most tightly linked one with a distance of 2.3 cM to RsDmR. These markers tightly linked to the RsDmR locus would facilitate marker-assisted selection and resistance gene pyramiding in radish breeding programs.

关键词: Raphanus sativus L. , molecular mapping , downy mildew , seedling-stage resistance , marker assisted selection

Abstract: Downy mildew (DM), caused by the fungus Peronospora parasitica, is a destructive disease of radish (Raphanus sativus L.) worldwide. Host resistance has been considered as an attractive and environmentally friendly approach to control the disease. However, the genetic mechanisms of resistance in radish to the pathogen remain unknown. To determine the inheritance of resistance to DM, F1, F2 and BC1F1 populations derived from reciprocal crosses between a resistant line NAU-dhp08 and a susceptible line NAU-qtbjq-06 were evaluated for their responses to DM at seedling stage. All F1 hybrid plants showed high resistance to DM and maternal effect was not detected. The segregation for resistant to susceptible individuals statistically fitted a 3:1 ratio in two F2 populations (F2(SR) and F2(RS)), and 1:1 ratio in two BC1F1 populations, indicating that resistance to DM at seedling stage in radish was controlled by a single dominant locus designated as RsDmR. A total of 1 972 primer pairs (1036 SRAP, 628 RAPD, 126 RGA, 110 EST-SSR and 72 ISSR) were screened, and 36 were polymorphic between the resistant and susceptible bulks, and consequently used for genotyping individuals in the F2 population. Three markers (Em9/ga24370, NAUISSR826700 and Me7/em10400) linked to the RsDmR locus within a 10.0 cM distance were identified using bulked segregant analysis (BSA). The SRAP marker Em9/ga24370 was the most tightly linked one with a distance of 2.3 cM to RsDmR. These markers tightly linked to the RsDmR locus would facilitate marker-assisted selection and resistance gene pyramiding in radish breeding programs.

Key words: Raphanus sativus L. , molecular mapping , downy mildew , seedling-stage resistance , marker assisted selection

XU Liang, JIANG Qiu-wei, WU Jian, WANG Yan, GONG Yi-qin, WANG Xian-li, Limera Cecilia　, LIU Li-wang. Identification and Molecular Mapping of the RsDmR Locus Conferring Resistance to Downy Mildew at Seedling Stage in Radish (Raphanus sativus L.)[J]. Journal of Integrative Agriculture, 2014, 13(11): 2362-2369.

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Identification and Molecular Mapping of the RsDmR Locus Conferring Resistance to Downy Mildew at Seedling Stage in Radish (Raphanus sativus L.)

Identification and Molecular Mapping of the RsDmR Locus Conferring Resistance to Downy Mildew at Seedling Stage in Radish (Raphanus sativus L.)

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