Genetic Analysis and Molecular Mapping of an All-Stage Stripe Rust Resistance Gene in Triticum aestivum-Haynaldia villosa Translocation Line V3

doi:10.1016/S2095-3119(13)60293-2

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (12): 2197-2208.DOI: 10.1016/S2095-3119(13)60293-2

Genetic Analysis and Molecular Mapping of an All-Stage Stripe Rust Resistance Gene in Triticum aestivum-Haynaldia villosa Translocation Line V3

HOU Lu, MA Dong-fang, HU Mao-lin, HE Miao-miao, LU Yan , JING Jin-xue

1.State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling 712100, P.R.China
2.Qinghai Academy of Agriculture and Forestry Science, Xining 810016, P.R.China
3.Department of Plant Pathology, China Agricultural University/Key Laboratory of Plant Pathology, Ministry of Agriculture, Beijing 100193,P.R.China

收稿日期:2012-11-12 出版日期:2013-12-01 发布日期:2013-12-17
通讯作者: JING Jin-xue, Tel: +86-29-87092434, E-mail: jingjinxue@163.com
作者简介:HOU Lu, E-mail: mantou428@163.com; MA Dong-fang, E-mail: madongfang1984@163.com
基金资助:
This research was supported by the 111 Project from the Education Ministry of China (B07049) and the Key Technologies R&D Program of China during the 11th Five- Year Plan period (2006BAD08A05) and the project of the Toxicity Variation of Wheat Stripe Rust Pathogen and Demonstration of Integrated Management of Stripe Rust, China (200903035-02).

Genetic Analysis and Molecular Mapping of an All-Stage Stripe Rust Resistance Gene in Triticum aestivum-Haynaldia villosa Translocation Line V3

HOU Lu, MA Dong-fang, HU Mao-lin, HE Miao-miao, LU Yan , JING Jin-xue

1.State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling 712100, P.R.China
2.Qinghai Academy of Agriculture and Forestry Science, Xining 810016, P.R.China
3.Department of Plant Pathology, China Agricultural University/Key Laboratory of Plant Pathology, Ministry of Agriculture, Beijing 100193,P.R.China

Received:2012-11-12 Online:2013-12-01 Published:2013-12-17
Contact: JING Jin-xue, Tel: +86-29-87092434, E-mail: jingjinxue@163.com
About author:HOU Lu, E-mail: mantou428@163.com; MA Dong-fang, E-mail: madongfang1984@163.com
Supported by:
This research was supported by the 111 Project from the Education Ministry of China (B07049) and the Key Technologies R&D Program of China during the 11th Five- Year Plan period (2006BAD08A05) and the project of the Toxicity Variation of Wheat Stripe Rust Pathogen and Demonstration of Integrated Management of Stripe Rust, China (200903035-02).

摘要/Abstract

摘要： Triticum aestivum-Hayaldia villosa translocation line V3 has shown effective all-stage resistance to the seven dominant pathotypes of Puccinia striiforms f. sp. tritici prevalent in China. To elucidate the genetic basis of the resistance, the segregating populations were developed from the cross between V3 and susceptible genotype Mingxian 169, seedlings of the parents and F2 progeny were tested with six prevalent pathotypes, including CYR29, CYR31, CYR32-6, CYR33, Sun11-4, and Sun11-11, F1 plants and F3 lines were also inoculated with Sun11-11 to confirm the result further. The genetic studied results showed that the resistance of V3 against CYR29 was conferred by two dominant genes, independently, one dominant gene and one recessive gene conferring independently or a single dominant gene to confer resistance to CYR31, two complementary dominant genes conferring resistance to both CYR32-6 and Sun11-4, two independently dominant genes or three dominant genes (two of the genes show cumulative effect) conferring resistance to CYR33, a single dominant gene for resistance to Sun11-11. Resistance gene analog polymorphism (RGAP) and simple-sequence repeat (SSR) techniques were used to identify molecular markers linked to the single dominant gene (temporarily designated as YrV3) for resistance to Sun11-11. A linkage map of 2 RGAP and 7 SSR markers was constructed for the dominant gene using data from 221 F2 plants and their derived F2:3 lines tested with Sun11-11 in the greenhouse. Amplification of the complete set of nulli-tetrasomic lines of Chinese Spring with a RGAP marker RG1 mapped the gene on the chromosome 1B, and then the linked 7 SSR markers located this gene on the long arm of chromosome 1B. The linkage map spanned a genetic distance of 25.0 cM, the SSR markers Xgwm124 and Xcfa2147 closely linked to YrV3 with genetic distances of 3.0 and 3.8 cM, respectively. Based on the linkage map, it concluded that the resistance gene YrV3 was located on chromosome arm 1BL. Given chromosomal location, the reaction patterns and pedigree analysis, YrV3 should be a novel gene for resistance to stripe rust in wheat. These closely linked markers should be useful in stacking genes from different sources for wheat breeding and diversification of resistance genes against stripe rust.

关键词: Haynaldia villosa , translocation line , stripe rust , RGAP-SSR , molecular mapping

Abstract: Triticum aestivum-Hayaldia villosa translocation line V3 has shown effective all-stage resistance to the seven dominant pathotypes of Puccinia striiforms f. sp. tritici prevalent in China. To elucidate the genetic basis of the resistance, the segregating populations were developed from the cross between V3 and susceptible genotype Mingxian 169, seedlings of the parents and F2 progeny were tested with six prevalent pathotypes, including CYR29, CYR31, CYR32-6, CYR33, Sun11-4, and Sun11-11, F1 plants and F3 lines were also inoculated with Sun11-11 to confirm the result further. The genetic studied results showed that the resistance of V3 against CYR29 was conferred by two dominant genes, independently, one dominant gene and one recessive gene conferring independently or a single dominant gene to confer resistance to CYR31, two complementary dominant genes conferring resistance to both CYR32-6 and Sun11-4, two independently dominant genes or three dominant genes (two of the genes show cumulative effect) conferring resistance to CYR33, a single dominant gene for resistance to Sun11-11. Resistance gene analog polymorphism (RGAP) and simple-sequence repeat (SSR) techniques were used to identify molecular markers linked to the single dominant gene (temporarily designated as YrV3) for resistance to Sun11-11. A linkage map of 2 RGAP and 7 SSR markers was constructed for the dominant gene using data from 221 F2 plants and their derived F2:3 lines tested with Sun11-11 in the greenhouse. Amplification of the complete set of nulli-tetrasomic lines of Chinese Spring with a RGAP marker RG1 mapped the gene on the chromosome 1B, and then the linked 7 SSR markers located this gene on the long arm of chromosome 1B. The linkage map spanned a genetic distance of 25.0 cM, the SSR markers Xgwm124 and Xcfa2147 closely linked to YrV3 with genetic distances of 3.0 and 3.8 cM, respectively. Based on the linkage map, it concluded that the resistance gene YrV3 was located on chromosome arm 1BL. Given chromosomal location, the reaction patterns and pedigree analysis, YrV3 should be a novel gene for resistance to stripe rust in wheat. These closely linked markers should be useful in stacking genes from different sources for wheat breeding and diversification of resistance genes against stripe rust.

Key words: Haynaldia villosa , translocation line , stripe rust , RGAP-SSR , molecular mapping

HOU Lu, MA Dong-fang, HU Mao-lin, HE Miao-miao, LU Yan , JING Jin-xue. Genetic Analysis and Molecular Mapping of an All-Stage Stripe Rust Resistance Gene in Triticum aestivum-Haynaldia villosa Translocation Line V3[J]. Journal of Integrative Agriculture, 2013, 12(12): 2197-2208.

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Genetic Analysis and Molecular Mapping of an All-Stage Stripe Rust Resistance Gene in Triticum aestivum-Haynaldia villosa Translocation Line V3

Genetic Analysis and Molecular Mapping of an All-Stage Stripe Rust Resistance Gene in Triticum aestivum-Haynaldia villosa Translocation Line V3

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