Identification and Phylogenetic Analysis of an Orf Virus Isolated from an Outbreak in Boer Goat in Shanxi Province

doi:10.1016/S1671-2927(11)60080-1

Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (6): 946-953.DOI: 10.1016/S1671-2927(11)60080-1

Identification and Phylogenetic Analysis of an Orf Virus Isolated from an Outbreak in Boer Goat in Shanxi Province

GU Shao-peng¹*, SHI Xin-tao¹*, SHI Zhong-yong², WANG Zhong-bing³ and ZHENG Ming-xue¹

1 College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, P.R.China
2 Genetically Modified Testing Center, Ministry of Agriculture/College of Life Science, Shanxi Agricultural Universit, Taigu 030801, P.R. China
3 Center for Animal Disease Control and Prevention of Shanxi Province, Taiyuan 030024, P.R.China

收稿日期:2011-06-10 修回日期:2011-06-10 出版日期:2011-06-20 发布日期:2011-06-03
通讯作者: ZHENG Ming-xue
作者简介:GU Shao-peng, Associate Professor, Tel: +86-354-6289229, E-mail: shpgu@163.com; Correspondence ZHENG Ming-xue, Professor, Tel: +86-354-6289229, E-mail: zhengmingxue288@sohu.com

Identification and Phylogenetic Analysis of an Orf Virus Isolated from an Outbreak in Boer Goat in Shanxi Province

GU Shao-peng¹*, SHI Xin-tao¹*, SHI Zhong-yong², WANG Zhong-bing³ and ZHENG Ming-xue¹

¹ College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, P.R.China
² Genetically Modified Testing Center, Ministry of Agriculture/College of Life Science, Shanxi Agricultural Universit, Taigu 030801, P.R. China
³ Center for Animal Disease Control and Prevention of Shanxi Province, Taiyuan 030024, P.R.China

Received:2011-06-10 Revised:2011-06-10 Online:2011-06-20 Published:2011-06-03
Contact: ZHENG Ming-xue
About author:GU Shao-peng, Associate Professor, Tel: +86-354-6289229, E-mail: shpgu@163.com; Correspondence ZHENG Ming-xue, Professor, Tel: +86-354-6289229, E-mail: zhengmingxue288@sohu.com

摘要/Abstract

摘要： To identify and analyze the Orf virus in Shanxi Province, China, an Orf virus strain was successfully isolated from crust materials of boer goat with clinical sore mouth symptom from a goat farm of Shanxi Province by passaging in lamb testis (LT). The Orf virus was identified by enzyme linked immunosorbent assay (ELISA) test, recurrent infection test, transmission electron microscopy, and PCR. The nucleotide and amino acid sequences of two genes of the Orf virus were analyzed.The results showed that under the electron microscopy the virus had a presence of typical parapoxvirus virions and there were many eosinophilic intracytoplasmic inclusions observed by hematoxylin-eosin (H&E) stain. In ELISA test, optical density (OD) readings of the sample showed a positive result, and the rabbits infected with the virus showed a typically Orf virus-infected appearance. All these findings proved that the sample was an Orf virus. The phylogenetic studies of Orf B2L and Orf F1L genes showed that the virus clustered in different branches and were closer to the Orf virus Nantou (DQ904351) and the OV-SA00 isolates (AY386264). Furthermore, the above results may provide some insight into the genotype of the etiological agent responsible for the Orf outbreak in Shanxi Province, and could also provide a comparative view of the B2L and F1L genes of parapoxvirus.

关键词: Orf virus, boer goat, identification, phylogenetic analysis, B2L, F1L

Abstract: To identify and analyze the Orf virus in Shanxi Province, China, an Orf virus strain was successfully isolated from crust materials of boer goat with clinical sore mouth symptom from a goat farm of Shanxi Province by passaging in lamb testis (LT). The Orf virus was identified by enzyme linked immunosorbent assay (ELISA) test, recurrent infection test, transmission electron microscopy, and PCR. The nucleotide and amino acid sequences of two genes of the Orf virus were analyzed.The results showed that under the electron microscopy the virus had a presence of typical parapoxvirus virions and there were many eosinophilic intracytoplasmic inclusions observed by hematoxylin-eosin (H&E) stain. In ELISA test, optical density (OD) readings of the sample showed a positive result, and the rabbits infected with the virus showed a typically Orf virus-infected appearance. All these findings proved that the sample was an Orf virus. The phylogenetic studies of Orf B2L and Orf F1L genes showed that the virus clustered in different branches and were closer to the Orf virus Nantou (DQ904351) and the OV-SA00 isolates (AY386264). Furthermore, the above results may provide some insight into the genotype of the etiological agent responsible for the Orf outbreak in Shanxi Province, and could also provide a comparative view of the B2L and F1L genes of parapoxvirus.

Key words: Orf virus, boer goat, identification, phylogenetic analysis, B2L, F1L

GU Shao-peng, SHI Xin-tao, SHI Zhong-yong, WANG Zhong-bing, ZHENG Ming-xue. Identification and Phylogenetic Analysis of an Orf Virus Isolated from an Outbreak in Boer Goat in Shanxi Province[J]. Journal of Integrative Agriculture, 2011, 10(6): 946-953.

GU Shao-peng, SHI Xin-tao, SHI Zhong-yong, WANG Zhong-bing and ZHENG Ming-xue. Identification and Phylogenetic Analysis of an Orf Virus Isolated from an Outbreak in Boer Goat in Shanxi Province[J]. Journal of Integrative Agriculture, 2011, 10(6): 946-953.

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Identification and Phylogenetic Analysis of an Orf Virus Isolated from an Outbreak in Boer Goat in Shanxi Province

Identification and Phylogenetic Analysis of an Orf Virus Isolated from an Outbreak in Boer Goat in Shanxi Province

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