Scientia Agricultura Sinica ›› 2008, Vol. 41 ›› Issue (2): 443-449 .doi: 10.3864/j.issn.0578-1752.2008.02.018

• PLANT PROTECTION • Previous Articles     Next Articles

Study on ELISA for quizalofop-p-ethyl

MingHua Wang HaiYan Shi Bo Li   

  1. 南京农业大学植物保护学院
  • Received:2006-05-14 Revised:1900-01-01 Online:2008-02-10 Published:2008-02-10

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Abstract: A hapten quizalofop acid was synthesized and covalently conjugated with the carrier proteins bovine serum albumin (BSA) and ovalbumin (OVA), respectively. The coupling ratio of Hatpen-BSA is 29.23 and the coupling ratio of Hatpen-OVA is 7.87. Polyclonal antibodies against quizalofop-p-ethyl were generated by immunizing rabbits with the hapten-protein conjugates (Hapten-BSA). Also antisera were screened and selected. The antibodies showed the IC50 for quizalofop-p-ethyl was 0.03495 靏/mL and the lower detection limit was 0.00192 靏/mL, the working range was assigned to concentrations 0.002-0.5 礸/mL for quizalofop-p-ethyl. Furthermore, other structurally closely related compounds tested hardly did cross-react in this assay. When water sample fortified with quizalofop-p-ethyl were analyzed, recoveries were in the range of 89.02-108.88%.

Key words: Quizalofop-p-ethyl, Hapten, Polyclonal antibody, IC-ELISA

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