Abstract: The Single nucleotide polymorphisms (SNPs) of 122 Nanyang cattle were detected for coding region of hypocretin receptor1 (HCRTR1) gene. Eleven SNP loci including 322 : G/A; 384:G/C; 420: T/C; 423: C/T; 481:T/A; 510:C/A; 627: T/C; 631:C/T; 690:G/A; 714:G/A and 736: C/T were found. The discovered SNPs were deposited in GenBank with accession No.: DQ981401, DQ986909, DQ986910, DQ986911, DQ986912, DQ986913, DQ986914, DQ901743, DQ986915, DQ986916 and DQ901742. The variation at 322，481，631 and 736 bp caused amino acid mutation，variation at the other 7 SNP loci were synonymity. Polymorphism information content(PIC), number of effective alleles(Ne) and heterozygosity(h) were also analyzed. three SNP loci (322 bp，510 bp and 736 bp) were moderate polymorphisms(0.3731, 0.3190 and 0.2851 respectively) while the left SNP loci showed low polymorphism(<0.25). The trends of heterozygosities were consistent with that of PIC. The SNP loci showed higher PIC as well as the higher heterozygosities.

Key words: Nanyang cattle, HCRTR1, Single nucleotide polymorphisms (SNPs)