冷藏和杀胚处理对米蛾卵扩繁松毛虫赤眼蜂效率的影响

doi:10.3864/j.issn.0578-1752.2026.18.006

Abstract

Abstract:

【Background】Efficient artificial propagation of egg parasitoids requires high quality alternative host eggs, so the fresh and nutritional states of host eggs are key factors to the propagation efficiency of egg parasitoids.【Objective】The Trichogramma dendrolimi, an egg parasitoid widely used in agricultural production, and its dominant alternative host Corcyra cephalonica eggs were used as test materials to explore the effects of refrigeration and embryo-killing on the quality of host eggs, so as to provide data support for scientific preservation of host eggs, optimization of artificial propagation technology of egg parasitoids, improvement of propagation efficiency and reduction of control costs.【Method】Four treatments of 1-day-old C. cephalonica eggs were performed: 4 ℃ refrigeration for 3 d followed by UV irradiation for embryo-killing (3 d+K), UV irradiation followed by 4 ℃ storage for 3 d (K+3 d), 4 ℃ storage for 7 d followed by UV irradiation (7 d+K), and UV irradiation followed by 4 ℃ storage for 7 d (K+7 d), with eggs only treated with UV irradiation for embryo-killing (K) as control. The parasitism rate of T. dendrolimi and the eclosion rate, female ratio, and developmental duration of the offspring parasitoids in eggs resulted from different treatments were compared. The contents of triglyceride, total protein, and trehalose in C. cephalonica eggs from each treatment were quantified, as well as the Wolbachia levels in both C. cephalonica eggs and T. dendrolimi emerged from the eggs, and their correlations with the biological indices of T. dendrolimi were analyzed.【Result】Compared with the control (K), the 7 d+K treatment significantly increased about 20% of the parasitism rate, and significantly increased about 72% of the offspring female amount of T. dendrolimi, without significant impact on eclosion rate and developmental duration of the offspring. The K+7 d treatment also could significantly increase 19% parasitism rate of the T. dendrolimi, but decreased the eclosion rate of the offspring wasps. Nutrient analysis showed that 4 ℃ storage (either before or after embryo-killing) significantly increased the triglyceride content in C. cephalonica eggs. Refrigeration treatment for 3 d before UV irradiation (3 d+K) significantly increased the protein content. None of the treatments significantly affected the trehalose content in host eggs. Correlation analysis revealed that triglyceride content in the eggs was significantly positively correlated with the parasitism rate of T. dendrolimi, the female proportion of its offspring and the Wolbachia level in the offspring wasps.【Conclusion】Pretreatment of 1-day-old C. cephalonica eggs with 7 d of refrigeration at 4 ℃ prior to embryo-killing under UV can effectively extend the duration of their freshness. This pretreatment may promote the parasitism efficiency and offspring female ratio of T. dendrolimi by increasing nutrient content, such as triglyceride, in host eggs. This study provides an optimized preservation and processing protocol for efficient and low-cost artificial mass rearing of T. dendrolimi using C. cephalonica eggs as an alternative host.

Key words: Trichogramma dendrolimi, Corcyra cephalonica egg, refrigeration, triglyceride, artificial mass rearing

SUN JiaNing, LIU ChenXi, LIU XiaoXia, LI Zhen. Effects of Refrigeration and Embryo-Killing Treatment of Corcyra cephalonica Eggs on Propagation Efficiency of Egg Parasitoid Trichogramma dendrolimi[J].Scientia Agricultura Sinica, 2026, 59(18): 4021-4032.

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处理 Treatment	寄生率 Parasitism rate (%)	羽化率 Eclosion rate (%)	雌蜂比 Female ratio (%)	发育历期 Developmental duration (d)
K	36.33±2.48c	87.62±3.63a	77.98±3.98a	10.85±0.08a
3 d+K	43.64±4.95abc	76.88±7.22ab	81.24±8.69a	10.36±0.15b
K+3 d	40.33±4.54bc	78.77±5.79ab	79.33±5.52a	10.30±0.15b
7 d+K	55.95±2.69a	83.53±2.97ab	90.50±1.13a	10.83±0.08a
K+7 d	54.58±2.99ab	71.30±3.55b	82.00±2.56a	10.39±0.09b

生物学指标 Biological indicator	相关性 Correlation	甘油三酯 Triglyceride	蛋白质 Protein	海藻糖 Trehalose
寄生率 Parasitism rate	相关系数ρ Correlation coefficient ρ	0.900*	0.700	0.100
寄生率 Parasitism rate	P值P value	0.037*	0.188	0.873
子代蜂羽化率 Offspring eclosion rate	相关系数ρ Correlation coefficient ρ	0.300	0.500	0.300
子代蜂羽化率 Offspring eclosion rate	P值P value	0.624	0.391	0.624
子代蜂雌蜂比 Offspring female ratio	相关系数ρ Correlation coefficient ρ	0.900*	0.700	0.100
子代蜂雌蜂比 Offspring female ratio	P值P value	0.037*	0.188	0.873
子代蜂发育历期 Offspring developmental duration	相关系数ρ Correlation coefficient ρ	0.100	0.300	0.100
子代蜂发育历期 Offspring developmental duration	P值P value	0.873	0.624	0.873
子代雌蜂数 Offspring female number	相关系数ρ Correlation coefficient ρ	0.900*	0.700	0.100
子代雌蜂数 Offspring female number	P值P value	0.037*	0.188	0.873

Effects of Refrigeration and Embryo-Killing Treatment of Corcyra cephalonica Eggs on Propagation Efficiency of Egg Parasitoid Trichogramma dendrolimi

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