Scientia Agricultura Sinica ›› 2024, Vol. 57 ›› Issue (18): 3601-3611.doi: 10.3864/j.issn.0578-1752.2024.18.007

• PLANT PROTECTION • Previous Articles     Next Articles

Effect of Fatty Acid Natural Product 2E, 4E-Decadienoic Acid on Tobacco Rhizosphere Microbial Communities

PENG ZhiXin1(), ZHANG XiFen1, HAN XiaoBin2(), SI GuoDong4, XU KangWen1, ZHANG ChengSheng1,3()   

  1. 1 Institute of Tobacco Research, Chinese Academy of Agricultural Sciences/National Agricultural Environmental Microbial Resource Bank (Shandong), Qingdao 266100, Shandong
    2 Guizhou Tobacco Company, Zunyi Company, Zunyi 562400, Guizhou
    3 Qingdao Agricultural Microbial Industry Technology Innovation Center, Qingdao 266100, Shandong
    4 Hailir Pesticides and Chemicals Group Co. Ltd., Qingdao 266000, Shandong
  • Received:2024-05-29 Accepted:2024-07-27 Online:2024-09-16 Published:2024-09-29
  • Contact: HAN XiaoBin, ZHANG ChengSheng

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Abstract:

【Objective】 The fatty acid compound 2E, 4E-decadienoic acid (DDA) was obtained from coculture of Bacillus subtilis Tpb55 and Trichoderma asperellum HG1, which has a specific inhibitory effect on Phytophthora. The objective of this study is to evaluate the effects of DDA on tobacco black shank (caused by P. nicotianae) and on the microbial community of tobacco rhizosphere soil, and to provide a basis for the application of such natural products in the prevention and control of plant Phytophthora disease. 【Method】 To evaluate the control efficacy of DDA against tobacco black shank, a pot experiment was performed, and the DNA copies of P. nicotianae in tobacco rhizosphere soil were detected by real-time fluorescence quantitative PCR technique. The bacterial, fungal and oomycetal communities in tobacco rhizosphere soil were analyzed through Illumina Hiseq high-throughput sequencing, and the correlation between soil nutrient factors and microbial community structure was assayed based on redundancy analysis and Mantel test. 【Result】 Compared with the control treatment with 0.5% DMSO, the treatment with 1.25 g·L-1 DDA for root irrigation showed 77.06% and 84.35% decrease for black shank disease index and P. nicotianae DNA copy number, respectively. DDA treatment also significantly increased the soil pH from 6.72 to 6.82, improved the soil conductivity by 102.11%, and decreased the soil organic matter by 13.05%. DDA treatment had no significant effect on the diversity and richness of fungi and bacteria in tobacco rhizosphere soil. However, the OTU level, Shannon index, Chao1 index, and ACE index of oomycetes significantly increased, while the Simpson index significantly decreased. The PCoA results showed that DDA treatment significantly affected the structure of tobacco rhizosphere oomycete community (r=0.667, P=0.028), but had no significant effect on fungi (r=0.259, P=0.305) and bacteria (r=0.593, P=0.098). The relative abundance of gram-positive bacterial genera such as Phycicoccus and Terrabacter significantly increased in DDA treatment, while the abundance of gram-negative bacterial genera including Devosia, Bordetella and Pseudoxanthomonas significantly decreased. Some beneficial fungi such as Aspergillus, Albifimbria, Arcopilus were enriched by DDA. For oomycetes, the relative abundance of Phytophthora was significantly decreased, but that of Globisporangium increased. The Mantel test results indicated that the changes in soil physicochemical properties caused by the application of DDA had no significant impact on the rhizosphere microbial community. 【Conclusion】2E, 4E-decadienoic acid (DDA), as a novel natural fatty acid compound, can effectively prevent and control tobacco black shank, reduce the pathogen DNA copy number in tobacco rhizosphere soil, and specifically regulate the structure of oomycete community. It has the application potential in the prevention and control of crop oomycete diseases.

Key words: Trichoderma asperellum, Bacillus subtilis, tobacco black shank, coculture, 2E, 4E-decadienoic acid (DDA), rhizosphere microbial community

Fig. 1

Effects of DDA on disease index of black shank (A) and DNA copy number of P. nicotianae in tobacco rhizosphere soil (B)"

Table 1

The physicochemical properties of tobacco rhizosphere soil affected by DDA"

处理
Treatment		 pH 电导率
Conductivity (μs‧cm-1)		 有机质
Organic matter (g‧kg-1)		 速效钾
AK
(mg‧L-1)		 铵态氮
NH4+-N
(mg‧kg-1)		 速效磷
AP
(mg‧kg-1)
空白土Blank soil 6.21±0.02c 395.67±4.91a 58.40±3.22c 0.246±0a 11.08±0.57b 0.093±0.01a
CK 6.72±0.03b 192.80±2.14b 75.16±2.31a 0.216±0.01b 31.98±1.06a 0.033±0b
DDA 6.82±0.03a 389.67±8.84a 65.35±0.90b 0.206±0bc 27.96±0.98a 0.042±0.01b

Table 2

Analysis of richness and diversity of bacteria, fungi and oomycetes in rhizosphere soil of different treatments"

微生物Microorganism 处理Treatment OTU Shannon Simpson Chao1 ACE
细菌
Bacteria		 CK 3688±127a 4.95±0.07a 0.01±0a 864.33±11.12a 4136.31±161.26a
DDA 3908±220a 4.98±0.02a 0.01±0a 860.67±8.96a 4344.10±231.48a
真菌
Fungi		 CK 992±57a 2.83±0.02a 0.10±0.02a 274.21±9.89a 1064.75±46.45a
DDA 1006±92a 2.75±0.02a 0.11±0.01a 259.82±8.86a 1081.89±92.46a
卵菌
Oomycetes		 CK 22±3b 0.19±0.02b 0.88±0.08a 21.00±1.04b 24.46±6.08b
DDA 38±3a 0.59±0.03a 0.70±0.05b 40.55±3.45a 42.34±5.70a

Fig. 2

PCoA of bacterial (A), fungal (B) and oomycetal (C) community at genus level"

Fig. 3

The bacterial (A), fungal (B) and oomycetal (C) community of tobacco rhizosphere soil at phylum level"

Fig. 4

Difference analysis of tobacco rhizosphere soil bacteria (A), fungi (B) and oomycetes (C) at genus level"

Fig. 5

RDA analysis between environmental factors and tobacco rhizosphere soil bacterial (A), fungal (B) and oomycetal (C) community"

Table 3

Mantel test of soil physicochemical properties and soil microbial community structure"

指标
Index		 细菌/相关系数（<BOLD>P</BOLD>值）
Bacteria/r (P value)		 真菌/相关系数（<BOLD>P</BOLD>值）
Fungi /r (P value)		 卵菌/相关系数（<BOLD>P</BOLD>值）
Oomycetes/r (P value)
电导率EC -0.218 (0.492) 0.576 (0.090) 0.151 (0.760)
pH -0.114 (0.740) -0.262 (0.449) 0.072 (0.884)
速效磷AP -0.312 (0.382) - 0.006 (0.982)
速效钾AK 0.042 (0.889) -0.063 (0.879) -0.209 (0.475)
铵态氮NH4+-N 0.103 (0.758) 0.199 (0.511) -0.455 (0.104)
有机碳SOC -0.358 (0.192) 0 (1) 0.276 (0.335)
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