Scientia Agricultura Sinica ›› 2018, Vol. 51 ›› Issue (1): 105-118.doi: 10.3864/j.issn.0578-1752.2018.01.010

• PLANT PROTECTION • Previous Articles     Next Articles

Fitness of Herbicide-Resistant BC3F4 between two herbicide-resistant transgenic Brassica napus and wild Brassica juncea

YAN Jing, WANG XiaoLei, ZHANG YuChi, ZHANG QingLing, WANG Jian, QIANG Sheng, SONG XiaoLing   

  1. College of Life Sciences, Nanjing Agricultural University, Nanjing 210095
  • Received:2017-06-12 Online:2018-01-01 Published:2018-01-01

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Abstract: 【Objective】 If herbicide-resistant transgenes escape from transgenic oilseed rape (Brassica napus, AACC, 2n=38) to their weedy relatives through pollen flow, weeds with the herbicide-resistant trait might produce new problems for weed control. Wild B. juncea (AABB, 2n=36), relative weed of transgenic B. napus, has become a major weed of crop systems across western China, and extended eastward along the Yangtze River valley. In order to provide experimental data for whether the transgene could successfully escape to wild B. juncea, it is necessary to evaluate the fitness of backcross generation between herbicide-resistant transgenic B. napus and wild B. juncea before it is released. 【Method】Wild B. juncea and herbicide-resistant BC3mF4R, BC3pF4R and BC3mF4L, BC3pF4L (R and L denote glyphosate- and glufosinate-resistant backcross progeny obtained with glyphosate- and glufosinate-resistant transgenic B. napus, respectively. m and p denote backcross progeny obtained with wild B. juncea as maternal plants and paternal plants, respectively) were planted at low (15 plants per plot) and high density (30 plants per plot) in pure and mixed stands (wild B. juncea﹕backcross generation were 4﹕1, 3﹕2 and 1﹕1). the vegetative components (plant height, stem diameter, the first branch number/plant, above-ground dry biomass/plant) and reproductive components (silique number/plant, total seed weight/plant, silique length, seed number/silique) of these backcross generations were measured. The differences of the composite fitness between BC3F4 and wild B. juncea were analyzed. 【Result】 Under pure stands, there was no significant difference in the fitness components and composite fitness of BC3F4R with that of wild B. juncea. The composite fitness of BC3F4L was also similar to wild B. juncea although the stem diameter, above-ground dry biomass/plant, and silique number/plant of BC3F4L at high density were significantly lower than that of wild B. juncea. Therefore, either glyphosate- or glufosinate-resistant BC3F4 was as fit as wild B. juncea regardless of density under pure stands. Under mixed stands, at low density, there was no significant difference in composite fitness of glyphosate- or glufosinate-resistant BC3F4 with that of wild B. juncea. At high density, there was no significant difference in fitness components and composite fitness of BC3F4R with that of wild B. juncea regardless planting proportions. However, the plant height, stem diameter, the first branch number/plant, above-ground dry biomass/plant, silique number/plant, total seed weight/plant and composite fitness of BC3F4L were significantly lower than that of wild B. juncea. The correlation analysis showed that the fitness components of BC3F4 were only related to the planting density. 【Conclusion】Glyphosate- or glufosinate- resistant BC3F4 between glyphosate- or glufosinate-resistant transgenic B. napus and wild B. juncea have a strong survival ability and potential possibility of establishing populations in the field. Moreover, the ecological risk of gene flow is higher from glyphosate-resistant transgenic B. napus than glufosinate-resistant transgenic B. napus. Therefore, preventing gene escape from herbicide-resistant B. napus to wild B. juncea should not only prevent their initial hybrids, but also should prevent the backcross between wild B. juncea and F1 or subsequent generations, so as not to produce higher fitness backcross generation.

Key words: glyphosate-resistant transgenic oilseed rape (Brassica napus), glufosinate-resistant transgenic oilseed rape (Brassica napus), wild Brassica juncea;backcross generations, fitness

[1]    国际农业生物技术应用服务组织. 2016年全球生物技术/转基因作物商业化发展态势. 中国生物工程杂志, 2017, 37(4): 1-8.
International Service for the Acquisition of Agri-biotech Applications. The global commercialization of biotech crops/transgenic crop in 2016. China Biotechnology, 2017, 37(4): 1-8. (in chinese)
[2]    International Service for the Acquisition of Agri-biotech Applications. http://www.isaaa.org/gmapprovaldatabase/advsearch/default.asp?CropID=2&TraitTypeID=1&DeveloperID=Any&CountryID=Any&ApprovalTypeID=Any[EB/OL]. [2017-09-26].
[3]    Warwick S I, Stewart C N. Crops come from wild plants: how domestication, transgenes, and linkage together shape ferality. Crop ferality and volunteerism, 2005, 36(1): 9-30.
[4]    Légère A. Risks and consequences of gene flow from herbicide- resistant crops: canola (Brassica napus L.) as a case study. Pest Management Science, 2005, 61: 292-300.
[5]    Pandolfo C E, Presotto A, Carbonell F T, Ureta S, Poverene M, Cantamutto M. Transgenic glyphosate-resistant oilseed rape (Brassica napus) as an invasive weed in Argentina: detection, characterization, and control alternatives. Environment Science and Pollution Research, 2016, 23: 24081-24091.
[6]    Yoshimura Y, Beckie H J, Matsuo K. Transgenic oilseed rape along transportation routes and port of Vancouver in western Canada. Environment Biosafety Research, 2006, 5(2): 67-75.
[7]    Knispel A L, McLachlan S M, Van Acker R C, Friesen L F. Gene flow and multiple herbicide resistance in escaped canola populations. Weed Science, 2008, 56: 72-80.  
[8]    Schafer M G, Ross A, Londo J P, Burdick C, Lee E H, Travers S E, Van de Water P K, Sagers C L. The establishment of genetically engineered canola populations in the U.S. PLoS One, 2011, 6(10): e25736.
[9]    Jørgensen R B, Andersen B. Spontaneous hybridization between oilseed rape (Brassica napus) and weedy B. campestris (Brassicaceae): a risk of growing genetically modified oilseed rape. American Journal of Botany, 1994, 81(12): 1620-1626.
[10]   Snow A A, Andersen B, Jørgensen R B. Costs of transgenic herbicide resistance introgressed from Brassica napus into weedy B. rapa. Molecular Ecology, 1999, 8(4): 605-615.
[11]   Bing D J, Downey R K, Rakow G F W. Hybridizations among Brassica napus, B. rapa and B. juncea and their two weedy relatives B. nigra and Sinapis arvensis under open pollination conditions in the field. Plant Breeding, 1996, 115: 470-473.
[12]   Liu Y B, Wei W, Ma K P, Darmency H. Backcrosses to Brassica napus of hybrids between B. juncea and B. napus as a source of herbicide-resistant volunteer-like feral populations. Plant Science, 2010, 179(5): 459-465.
[13]   Eber F, Chèvre A M, Baranger A, Vall6e P, Tanguy X, Renard M. Spontaneous hybridization between a male-sterile oilseed rape and two weeds. Theoretical and Applied Genetics, 1994, 88: 362-368.
[14]   Darmency H, Lefol E, Fleury A. Spontaneous hybridizations between oilseed rape and wild radish. Molecular Ecology, 1998, 7(11): 1467-1473.
[15]   Gueritaine G, Sester M, Eber F, Chèvre A M, Darmency H. Fitness of backcross six of hybrids between transgenic oilseed rape (Brassica napus) and wild radish (Raphanus raphanistrum). Molecular Ecology, 2002, 11(8): 1419-1426.
[16]   Hall L, Topinka K, Huffman J, Davis L, Good A. Pollen flow between herbicide-resistant Brassica napus is the cause of multiple-resistant B. napus volunteers. Weed Science, 2000, 48: 688-694.
[17]   Aono M, Wakiyama S, Nagatsu M, Kaneko Y, Nishizawa T, Nakajima N, Tamaoki M, Kubo A, Saji H. Seeds of a possible natural hybrid between herbicide-resistant Brassica napus and Brassica rapa detected on a riverbank in Japan. Genetically Modified Crops, 2011, 2(3): 201-210.
[18]   Jenczewski E, Ronfort J, Chèvre A M. Crop-to-wild gene flow, introgression and possible fitness effects of transgenes. Environmental Biosafety Researeh, 2003, 2: 9-24.
[19]   Mikkelsen T R, Andersen B, Jorgensen R B. The risk of crop transgene spread. Nature, 1996, 380: 31.
[20]   Hauser T P, Shaw R G, Østergård H. Fitness of F1 hybrids between weedy Brassica rapa and oilseed rape (B. napus). Heredity, 1998, 81(4): 429-435.
[21]   Hauser T P, Jørgensen R B, Østergård H. Fitness of backcross and F2 hybrids between weedy Brassica rapa and oilseed rape (B. napus). Heredity, 1998, 81(4): 436-443.
[22]   Hauser T P, Damgaard C, Jørgensen R B. Frequency- dependent fitness of hybrids between oilseed rape (Brassica napus) and weedy B. rapa (Brassicaceae). American Journal of Botany, 2003, 90(4): 571-578.
[23]   Vacher C, Weis A E, Hermann D, Kossler T, Young C, Hochberg M E. Impact of ecological factors on the initial invasion of Bt transgenes into wild populations of birdseed rape (Brassica rapa). Theoretical and Applied Genetics, 2004, 109: 806-814.
[24]   Chèvre A M, Eber F, Baranger A,Renard M. Gene flow from transgenic crops. Nature, 1997, 389(6654): 924.
[25]   Chèvre A M, Eber F, Baranger A, Hureau G, Barret P, Picault H, Renard M. Characterization of backcross generations obtained under field conditions from oilseed rape-wild radish F1 interspecific hybrids: an assessment of transgene dispersal. Theoretical and Applied Genetics, 1998, 97(1/2): 90-98.
[26]   Mercer K L, Andow D A, Wyse D L,Wyse D L, Shaw R G. Stress and domestication traits increase the relative fitness of crop-wild hybrids in sunflower. Ecology Letters, 2007, 10: 383-393.
[27]   Huangfu C, Qiang S, Song X. Performance of hybrids between transgenic oilseed rape (Brassica napus) and wild Brassica juncea: An evaluation of potential for transgene escape. Crop Protection, 2011, 30(1): 57-62.
[28]   Campbell D R, Waster N M. Genotype-by-environment interaetion and the fitness of plant hybrids in the wild. Evolution, 2001, 55(4): 669-676.
[29]   Simard M J, Légère A, Séguin-Swartz G, Nair H, Warwick S. Fitness of double vs. single herbicide-resistant canola. Weed Science, 2005, 53: 489-498.
[30]   Campbell L G, Snow A A, Ridley C E. Weed evolution after crop gene introgression: greater survival and feeundity of hybrids in a new environment. Ecology Letters, 2006, 9: 1198-1209.
[31]   Londo J P, Bautista N S, Sagers C L, Lee E H, Watrud L S. Glyphosate drift promotes changes in fitness and transgene gene flow in canola (Brassica napus) and hybrids. Annals of Botany, 2010, 106(6): 957-965.
[32]   Huangfu C H, Song X L, Qiang S. ISSR variation within and among wild Brassica juncea populations: implication for herbicide resistance evolution. Genetic Resources and Crop Evolution, 2009, 56(7): 913-924.
[33]   浦惠明, 戚存扣, 张洁夫, 傅寿仲, 高建芹, 陈新军, 陈松, 赵祥祥. 转基因抗除草剂油菜对近缘作物的基因漂移. 生态学报, 2005, 25(3): 581-588.
Pu H M, Qi C K, Zhang J F, Fu S Z, Gao J Q, Chen X J, Zhao XＸ. Studies on the gene flow from herbicide-tolerant GM rapeseed to its close relative crops. Acta Ecologica Sinica, 2005, 25(3): 581-588. (in chinese)
[34]   宋小玲, 皇甫超河, 强胜. 抗草丁膦和抗草甘膦转基因油菜的抗性基因向野芥菜的流动. 植物生态学报, 2007, 31(4): 729-737.
Song X L, Huangfu C H, Qiang S. Gene flow from glufosinate or glyphosate-torelant oilseed rape to wild rape. Journal of Plant Ecology, 2007, 31(4): 729-737. (in chinese)
[35]   Song X L, Wang Z, Zuo J, Huangfu C H, Qiang S. Potential gene flow of two herbicide-tolerant transgenes from oilseed rape to wild B. juncea var. gracilis. Theoretical and applied genetics, 2010, 120(8): 1501-1510.
[36]   郑爱琴, 强胜, 宋小玲. 抗除草剂转基因油菜与野芥菜的杂交1代与5种常规栽培油菜回交后代的适合度. 应用与环境生物学报, 2014, 20(3): 337-344.
Zheng A Q, Qiang S, Song X L. Fitness of backcross between F1 (wild B. juncea × herbicide-resistant transgenic oilseed rape) and 5 conventional cultivate varieties. Chinese Journal of Applied and Environmental Biology,2014, 20(3): 337-344. (in chinese)
[37]   Liu Y B, Darmency H, Jr Stewart C N, Wei W, Tang Z X, Ma K P. The effect of Bt-transgene introgression on plant growth and reproduction in wild Brassica juncea. Transgenic Research, 2015, 24(3): 537-547.
[38]   张庆玲, 王建, 强胜, 王晓蕾, 闫静, 伏建国, 宋小玲. 抗草甘膦转基因油菜与野芥菜回交3代子1代和子2代的适合度研究. 南京农业大学学报, 2017, 40(3): 434-443.
Zhang Q L, Wang J, Qiang S, WANG X L, Yan J, Fu J G, Song X L. Fitness of BC3F2 and BC3F3 between glyphosate-resistant transgenic oilseed rape and wild Brassica juncea. Journal of Nanjing Agricultural University, 2017, 40(3): 434-443. (in chinese)
[39]   Rieger M A, Lamond M, Preston C, Powles S B, Roush R T. Pollen-mediated movement of herbicide resistance between commercial canola fields. Science, 2002, 296(5577): 2386-2388.
[40]   Haygood R, Ives A R, Andow D A. Consequences of recurrent gene flow from crops to wild relatives. Proceedings of the Royal Society of London B: Biological Sciences, 2003, 270: 1879-1886.
[41]   Moon H S, Halfhill M D, Good L L, Raymer P L, Jr Stewart C N. Characterization of directly transformed weedy Brassica rapa and introgressed B. rapa with Bt cry1Ac and gfp genes. Plant Cell Reports, 2007, 26: 1001-1010.
[42]   Wang W, Xia H, Yang X, Xu T, Si HJ, Cai XX, Wang F, Su J, Snow A A, Lu B R. A novel 5-enolpyruvoylshikimate-3-phosphate (EPSP) synthase transgene for glyphosate resistance stimulates growth and fecundity in weedy rice (Oryza sativa) without herbicide. New Phytologist, 2014, 202: 679-688.
[43]   Wilkinson M, Tepfer M. Fitness and beyond: preparing for the arrival of GM crops with ecologically important novel characters. Environmental Biosafety Research, 2009, 8: 1-14.
[44]   Landbo L, Jørgensen R B. Seed germination in weedy Brassica campestris and its hybrids with B. napus: Implications for risk assessment of transgenic oilseed rape. Euphytica, 1997, 97(2): 209-216.
[45]   Roach D A, Wulff R D. Maternal effects in plants. Annual Review of Ecology and Systematics, 1987, 18: 209-235.
[46]   Chèvre A M, AdameZyk K, Eber F, Huteau V, Coriton O, Letanneur J C, Laredo C, Jenczewski E, Monod H. Modelling gene flow between oilseed rape and wild radish. Ⅰ. Evolution of ehromosome strueture. Theoretieal and Applied Geneties, 2007, 114: 209-221.
[47]   Halfhill M D, Sutherland J P, Moon H S, Poppy G M, Warwick S, Weissinger A, Rufty T, Raymer P L, Jr Stewart C N. Growth, productivity, and competitiveness of introgressed weedy Brassica rapa hybrids selected for the presence of Bt cry1Ac and gfp transgenes. Molecular Ecology, 2005, 14(10): 3177-3189.
[48]   马霓, 张春雷, 李俊, 李光明. 种植密度对直播油菜结实期源库关系及产量的调节. 中国油料作物学报, 2009, 31(2): 180-184.
Ma N, Zhang C L, Li J, Li G M. Regulation of planting density on source-sink relationship and yield at seed-set stage of rapeseed (Brassica napus L.). Chinese journal of oil crop sciences, 2009, 31(2): 180 -184. (in Chinese) 
[49]   邵留东. 不同播期条件下种植密度对油菜生长发育及产量的影响[D]. 武汉: 华中农业大学, 2009.
Shao L D. Effects of planting density on the development and yield of rapeseed (Brassica napus L.) under different sowing dates[D]. Wuhan: Huazhong Agricultural University, 2009. (in Chinese) 
[50]   Johannessen M M, Damgaard C, Andersen B A, Jørgensen R B. Competition affects the production of first backcross offspring on F1-hybrids, Brassica napus × B. rapa. Euphytica, 2006, 150: 17-25.
[51] Devos Y, Hails R S, Messéan A, Perry J N, Squire G R. Feral genetically modified herbicide tolerant oilseed rape from seed import spills: are concerns scientifically justified? Transgenic Research, 2012, 21(1): 1-21.
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