Scientia Agricultura Sinica ›› 2017, Vol. 50 ›› Issue (19): 3828-3836.doi: 10.3864/j.issn.0578-1752.2017.19.019

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles    

Research and Application of Production Technology of Natural Honeybee Bread

JIANG WuJun1,WU XiaoBo1, LIU GuangNan2, HE XuJiang1, YAN WeiYu1, ZENG ZhiJiang1   

  1. 1Honeybee research institute, Jiangxi Agricultural University, Nanchang 330045; 2Bureau of Agriculture and Food in Shicheng county of Jiangxi Province, Shicheng 342700, Jiangxi
  • Received:2017-03-20 Online:2017-10-01 Published:2017-10-01

Abstract: 【objective】 Honeybee bread is an important food resource for honeybee old larvae and young bees, and contains full nutrition essentially for larval development. It is also edible for humans and has promising commercial prospects. However, the lack of technology has limited the large-scale harvest of honeybee bread. The objective of this study is to develop a device for quick harvesting high quality honeybee bread and provide a technical support for the future mechanized production of honeybee bread.【Method】This study developed a honeybee bread producing instrument (HBPI) including bee bread storage and collecting devices, according to honeybee bread-storing biological characteristics. In the lotus blooming season, 5 strong honeybee Apis mellifera colonies were selected as the experimental materials to explore the practicability of this technology. Each colony was placed with a HBPI. The completely fermented bee bread was collected, and was separated by the bee bread collecting device (HBPI bee bread). Plastic pollen traps were employed for collecting the fresh lotus pollen using the same 5 colonies (fresh pollen). The completely fermented bee bread from the natural frames from these colonies was also excavated (natural bee bread). Furthermore, the quality of fresh lotus pollen and honeybee bread from natural honeybee frames and HBPI was compared, respectively, by analyzing pH, water activity, moisture content, pollen particles activity, enzyme activity and content of amino acids by using pH meter, water activity detector, directly aesiccation method, triphenyl tetrazolium chloride method, Marklund method, ultraviolet spectrophotometry and amino acid analyser, respectively. 【Result】 Honeybees successfully stored honeybee bread into the HBPI and maintained its original shape. The pH, water activity, moisture content, pollen particles activity, catalase and superoxide dismutase activity of fresh lotus pollen were significantly higher than natural and HBPI honeybee bread. There was no significant difference between fresh lotus pollen and two types of honeybee bread in terms of total free amino acids and hydrolysis amino acids. However, the valine, isoleucine, leucine, tyrosine, histidine, arginine in free amino acids, and the aspartate, proline in hydrolysis amino acids were significantly higher in two honeybee bread categories compared to fresh pollen. There was no significant difference between the natural and HBPI honeybee bread in physicochemical property and collectivity nutrient composition. 【Conclusion】 This study developed a honeybee bread producing instrument and confirmed its good practicability, which could be widely applied in further apiculture.

Key words: honeybee pollen, honeybee bread, honeybee bread producing instrument, nutritional components

[1]    江武军, 曾志将. 蜂粮研究进展. 黑龙江畜牧兽医, 2015(2): 118-119.
JIANG W J, ZENG Z J. Advances on the bee bread research. Heilongjiang Animal Science and veterinary Medicine, 2015(2): 118-119. (in Chinese)
[2]    LINSKENS H F, JORDE W. Pollen as food and medicine: A review. Economic Botany, 1997, 51(1): 78-86.
[3]    HUANG Z. Honey bee nutrition. American Bee Journal, 2010, 150(8): 773-776.
[4]    MOGREN C L, LUNDGREN J G. Neonicotinoid-contaminated pollinator strips adjacent to cropland reduce honey bee nutritional status. Scientific Reports, 2016, 6: 29608.
[5]    ANDJELKOVIC B, JEVTIC G, MARKOVIC J, MLADENOVIC M, PSEVA V. Quality of honey bee bread collected in spring. Proceedings of the International Symposium on Animal Science, 2014, 9: 450-454.
[6]    BASUALDO M, BARRAGAN S, ANTUNEZ K. Bee bread increases honeybee haemolymph protein and promote better survival despite of causing higher Nosema ceranae abundance in honeybees. Environmental Microbiology Reports, 2014, 6(4): 396-400.
[7]    WILLARD L E, HAYES A M, WALLRICHS M A, RUEPPELL O. Food manipulation in honeybees induces physiological responses at the individual and colony level. Apidologie, 2011, 42(4): 508-518.
[8]    李忠谱. 浅谈蜂粮. 蜜蜂杂志, 1989(5): 5-6.
LI Z P. Talk shallowly of bee bread. Journal of Bee, 1989(5): 5-6. (in Chinese)
[9]    袁耀东. 蜂粮的成分和作用. 中国养蜂, 1991(6): 36-37.
YUAN Y D. The composition and function of bee bread. Apiculture of China, 1991(6): 36-37. (in Chinese)
[10]   苏松坤, 陈盛禄, 林雪珍, 胡福良, 余旭平, 汪礼国, 王正二. 茶 (Camellia sinensis)蜂花粉与蜂粮中花粉形态和营养成分的比较. 中国养蜂, 2000, 51(6): 3-6.
SU S K, CHEN S L, LIN X Z, HU F L, YU X P, WANG L G, WANG Z E. The comparison of pollen morpha and nutrient components between corbicular tea (Camellia sinensis) pollen and bee bread. Apiculture of China, 2000, 51(6): 3-6. (in Chinese)
[11]   苏松坤, 陈盛禄. 茶 (Camellia sinensis)蜂花粉及其蜂粮的营养成分研究. 上海交通大学学报 (农业科学版), 2002, 20(2): 95-99.
SU S K, CHEN S L. The research of nutrient compositions of tea (Camellia sinensis) corbicular pollen and tea bee bread. Journal of Shanghai Jiao Tong University (Agricultural Science), 2002, 20(2): 95-99. (in Chinese)
[12]   苏松坤. 蜂粮的酿制机理和营养价值的研究[D]. 杭州: 浙江大学, 2000.
SU S K. Studies on brew mechanism and nutritive value of bee bread[D]. Hangzhou: Zhejiang University, 2000. (in Chinese)
[13] LOPER G M, STANDIFER L N, THOMPSON M J, GILLIAM M. Biochemistry and microbiology of bee-collected almond (Prunus dulcis) pollen and bee bread.Ⅰ. Fatty acids, sterols, vitamins and minerals. Apidologie, 1980, 11(1): 63-73.
[14]   CRANE E. Bees and Beekeeping: Science, Practice and World Resources. Cornell University Press, 1990.
[15]   KRELL R. Value-added products from beekeeping. Fao Agricultural Services Bulletin, 1996, 36(5): 229-234.
[16]   张少斌. 分合式巢房巢脾: CN99222971.5[P]. 2000-05-31[2017- 04-25].
ZHANG S B. Split type of comb: CN99222971.5[P]. 2000-05-31 [2017-04-25]. (in Chinese)
[17]   张少斌, 张明海. 开合式巢脾: CN200510009624.0[P]. 2006-07-26 [2017-04-25].
ZHANG S B, ZHANG M H. Retractable comb: CN200510009624. 0[P]. 2006-07-26[2017-04-25]. (in Chinese)
[18]   张少斌, 张石胜. KF-1、2型塑料巢脾在养蜂生产上的应用. 中国养蜂, 1999, 50(4): 17.
ZHANG S B, ZHANG S S. The application in the production of plastic comb bee of KF-1 or KF-2. Apiculture of China, 1999, 50(4): 17. (in Chinese)
[19]   苏松坤, 陈盛禄, 林雪珍, 胡福良. 蜂粮生产技术. 养蜂科技, 2000(3): 29-30.
SU S K, CHEN S L, LIN X Z, HU F L. Bee bread production technology. Apicultural Science and Technology, 2000(3): 29-30. (in Chinese)
[20]   苏松坤, 陈盛禄, 刘华, 林雪珍. 天然蜂粮生产方法. 养蜂科技, 2006(6): 32-33.
SU S K, CHEN S L, LIU H, LIN X Z. Method for producing natural bee bread. Apicultural Science and Technology, 2006(6): 32-33. (in Chinese)
[21]   苏松坤, 陈盛禄. 天然蜂粮的生产方法及器具: CN200410073451. 4[P]. 2005-06-08[2017-04-25].
SU S K, CHEN S L. Method and apparatus for producing natural bee bread: CN200410073451.4[P]. 2005-06-08[2017-04-25]. (in Chinese)
[22]   AKHMETOVA R, SIBGATULLIN J, GARMONOV S, AKHMETOVA L. Technology for extraction of bee-bread from the honeycomb. Procedia Engineering, 2012, 42: 1822-1825.
[23]   苏松坤, 陈盛禄, 余旭平, 林雪珍, 胡福良. 蜂粮中的细菌对pH的影响. 中国养蜂, 2002, 53(2): 4-5.
SU S K, CHEN S L, YU X P, LIN X Z, HU F L. The influence of bacteria isolated from bee bread on pH. Apiculture of China, 2002, 53(2): 4-5. (in Chinese)
[24]   中华人民共和国国家质量监督检验检疫总局. 食品水分活度的测定: GB/T 23490-2009[S]. 2009-05-01[2017-04-25].
General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China. Determination of water activity in foods: GB/T 23490-2009[S]. 2009-05-01[2017-04-25]. (in Chinese)
[25]   中华人民共和国卫生部. 食品中水分的测定: GB 5009.3-2010[S]. 2010-06-01[2017-04-25].
Ministry of Health of the People’s Republic of China. Determination of moisture in foods: GB 5009.3-2010[S]. 2010-06-01[2017-04-25]. (in Chinese)
[26]   刘意秋. 蜂学专业综合实验教程. 北京: 中国农业出版社, 2014: 113.
LIU Y Q. Comprehensive experimental course of bee Science. Beijing: China Agriculture Press, 2014: 113. (in Chinese)
[27]   中华人民共和国卫生部. 保健食品中超氧化物歧化酶(SOD)活性的测定: GB/T 5009.171-2003[S]. 2004-01-01[2017-04-25].
Ministry of Health of the People’s Republic of China. Determination of the action of superoxide dismutase in health foods: GB/T 5009.171-2003[S]. 2004-01-01[2017-04-25]. (in Chinese)
[28]   中华人民共和国国家质量监督检验检疫总局. 蜂花粉中过氧化氢酶的测定方法——紫外分光光度法: GB/T 23195-2008[S]. 2009-06-01[2017-04-25].
General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China. Method for the determination of catalase in bee pollen—Ultaraviolet spectrophotometry: GB/T 23195-2008[S]. 2009-06-01[2017-04-25]. (in Chinese)
[29]   中华人民共和国卫生部. 食品中氨基酸的测定: GB/T 5009.124-2003[S]. 2004-01-01[2017-04-25].
Ministry of Health of the People’s Republic of China. Determination of amino acids in foods: GB/T 5009.124-2003[S]. 2004-01-01 [2017-04-25]. (in Chinese)
[30]   费起充. 蜂粮的制作. 蜜蜂杂志, 1998(7): 15.
FEI Q C. Bee bread production. Journal of Bee, 1998(7): 15. (in Chinese)
[31]   VASQUEZ A, OLOFSSON T C. The lactic acid bacteria involved in the production of bee pollen and bee bread. Journal of Apicultural Research and Bee World, 2009, 48(3): 189-195.
[32]   苏松坤, 陈盛禄, 李言清. 人工蜂粮的制备方法: CN03150405.1[P]. 2004-04-14[2017-04-25].
SU S K, CHEN S L, LI Y Q. The preparation method of artificial bee bread: CN03150405.1[P]. 2004-04-14[2017-04-25]. (in Chinese)
[33]   DUAN C R, FENG Y F, ZHOU H, XIA X H, SHANG Y N, CUI Y M. Optimization of fermentation condition of man-made bee-bread by response surface methodology//ZHANG T C, NAKAJIMA M. Advances in Applied Biotechnology. Springer Berlin Heidelberg, 2015, 333: 353-363.
[34]   崔学沛. 意大利蜜蜂天然成熟蜂粮菌种鉴定及人工蜂粮发酵影响因素的探究[D]. 泰安: 山东农业大学, 2015.
CUI X P. The identification of strains in natural mature bee bread of honey bee (A. mellifera L.) and study on the fermentation conditions of artificial bee bread[D]. Taian: Shandong Agricultural University, 2015. (in Chinese)
[35]   苏松坤, 陈盛禄. 蜂粮酿制过程中花粉粒活力和水活度的测定. 中国养蜂, 2002, 53(5): 8-9.
SU S K, CHEN S L. Determination of water activity and pollen particles activity during the process from corbicular pollen to bee bread. Apiculture of China, 2002, 53(5): 8-9. (in Chinese)
[36]   ISIDOROV V A, ISIDOROVA A G, SCZCZEPANIAK L, CZYZEWSKA U. Gas chromatographic-mass spectrometric investigation of the chemical composition of beebread. Food Chemistry, 2009, 115(3): 1056-1063.
[37]   ANDERSON K E, CARROLL M J, SHEEHAN T, MOTT B M. MAES P, CORBY-HARRIS V. Hive-stored pollen of honey bees: many lines of evidence are consistent with pollen preservation, not nutrient conversion. Molecular Ecology, 2014, 23: 5904-5917.
[38]   HUmAN H, NICOLSON S W. Nutritional content of fresh, bee-collected and stored pollen of Aloe greatheadii var. davyana (Asphodelaceae). Phytochemistry, 2006, 67(14): 1486-1492.
[39]   DEGRANDI-HOFFMAN G, ECKHOLM B J, HUANG M H. A comparison of bee bread made by Africanized and European honey bees (Apis mellifera) and its effects on hemolymph protein titers. Apidologie, 2013, 44(1): 52-63.
[40]   AUDISO M C, TERZOLO H R, APELLA M C. Bacteriocin from honeybee beebread Enterococcus avium, active against Listeria monocytogenes. Applied & Environmental Microbiology, 2005, 71(6): 3373-3375.
[41]   NAGAI T, NAGASHIMA T, MYODA T, INOUE R. Preparation and functional properties of extracts from bee bread. Die Nahrung, 2004, 48(3): 226-229.
[42]   HABRYKA C, KRUCZEK M, DRYGAS B. Bee products used in apitherapy. World Scientific News, 2016, 48: 254-258.
[43]   CEKSTERYTE V, NAVAKAUSKIENE R, TREIGYTE G, JANSEN E, KURTINAITIENE B, DABKEVICIENE G, BALZEKAS J. Fatty acid profiles of monofloral clover beebread and pollen and proteomics of red clover (Trifolium pratense) pollen. Bioscience, Biotechnology, and Biochemistry, 2016, 80(11): 2100-2108.
[44]   WONG K H, AZIZ S A, MOHAMED S. Sensory aroma from Maillard reaction of individual and combinations of amino acids with glucose in acidic conditions. International Journal of Food Science & Technology, 2008, 43(9): 1512-1519.
[1] LI Zhen,LIU ZhiYong,JIANG WuJun,HE XuJiang,YAN WeiYu,ZHANG LiZhen,ZENG ZhiJiang. Effects of Natural Bee Bread on Blood Lipid, Antioxidation and Immune Function in Rats with Hyperlipidemia [J]. Scientia Agricultura Sinica, 2019, 52(16): 2912-2920.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!