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Rapid Detection Technologies for Potential Crop Invasive Diseases

FENG Jie; ZHENG Xiao-bo; CHEN Wan-quan; KANG Zhen-sheng; WANG Xiao-ming; WANG Yuan-chao; HU Bai-shi; ZHANG Guo-zhen; HAN Li-juan   

  1. 1、State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, CAAS, Beijing 100094;
    2、The Key Laboratory of Monitoring and Management of Plant Diseases and Insects of Chinese Ministry of Agriculture; College of Plant Protection, Nanjing Agricultural University, Nanjing 210095;
    3、College of Plant Protection, Northwest Agriculture & Forestry University, Yangling 712100;
    4、Institute of Crop Sciences, CAAS, Beijing 100081;
    5、College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094;
    6、Beijing Entry and Exit Inspection and Quarantine Bureau, Beijing 100025
  • Online:2007-12-31 Published:2007-12-31

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Abstract: For developing rapid detection technologies of alien invasive diseases of TCK, pear fire blight and soybean Phytophthora root rot, associated research were carried out with the support of National Program on Key Basic Research Projects (973 Program): “Invasive Mechanisms and Control Basic Research of Agricultural and Forest Alien Invasive Species” (No.2002CB111400). SCAR marker for rapid and sensitive detection of TCK was obtained. Sensitive molecular detection method of Erwinia amylovora with “One step and duplex PCR” based on chromosomal and pEA29 plasmid sequences and detection protocol of identification in asymptomatic samples infected by E. amylovora based on Bio-PCR habe been developed. It is indicated that wide genetic variations were occurred in the long history of interaction between Phytophthora sojae populations and soybean varieties in different areas in China. A simple and effective method of isolating P. sojae from soils and molecular detection system of soybean Phytophthora root rot based on ITS sequences were established. The protocol was determined to efficiently detect pathogen at a theoretical rate of half spore. The Kits for detecting pear fire blight, TCK and soybean Phytophthora root rot based on our research results have been exploited.

Key words: National Basic Research and Development Program (973 program) , Crop invasive diseases , Rapid molecular detection , TCK , Pear fire blight , Soybean Phytophthora root rot

[1]万方浩, 郭建英, 王德辉. 中国外来入侵生物的危害与管理对策. 生物多样性, 2002, 10: 119-125.
[2]程峻峰, 万方浩, 郭建英, 张国安. 外来有害入侵生物-西花蓟马. 中国生物防治, 2005, 21: 74-79.
[3]朱振东, 王化波, 王晓鸣. 一种土壤中大豆疫霉菌分离新方法. 菌物系统, 2003, 22: 142-147.
[4]王子迎, 王源超, 张正光, 郑小波. 土壤中大豆疫霉菌诱捕方法的改进. 植物病理学报, 2005, 35: 557-559.
[5]韩丽娟, 刘文洪. 大豆疫霉病及其遗传多样性的研究方法. 分子植物育种, 2004, 2: 877-883.
[6]梁  宏, 彭友良, 张国珍, 陈万权, 刘太国. 腥黑粉菌属3种检疫性真菌rDNA-IGS区的扩增及其序列分析. 植物病理学报, 2006, 36: 407-412.
[7]梁  宏, 张国珍, 陈万权, 刘太国. 小麦矮腥黑穗病菌与其近源种的rDNA-ITS序列分析. 植物病理学报, 2005, 35: 181-183.
[8]Chen W Q, Liu J H. Development of a species-specific SCAR marker for detection of Tilletia controversa Kuhn, the causal agent of dwarf bunt of wheat. Proceedings of the 15th International Plant Protection Congress, Beijing, 2004: 365.
[9]Chen W Q, Liu T G, Liu J H, Xu S C. DNA marker of Tilletia controversa Kuhn, a causal agent of wheat dwarf bunt. Proceedings of XVth Biennial Workshop on the Smut Fungi, Czech. 2006.
[10]胡白石, 卢  玲, 刘凤权, 许志刚, 陈建东, 焦国尧. 利用间接免疫荧光染色和协同凝集技术检测梨火疫病菌. 南京农业大学学报, 2003, 26: 41-45.
[11]周国梁, 胡白石, 印丽萍, 李尉民, 许志刚. 利用Monte-Carlo模拟再评估梨火疫病病菌随水果果实的入侵风险. 植物保护学报, 2006, 33: 47-50.
[12]陈  晨, 陈  娟, 胡白石, 白艺珍, 姜英华, 刘凤权. 梨火疫病菌在中国的潜在分布及入侵风险分析. 中国农业科学, 2007, 40: 940-947.
[13]钱国良, 胡白石, 卢  玲, 刘凤权, 许志刚. 梨火疫病菌的实时荧光PCR检测. 植物病理学报, 2006, 36: 123-128.
[14]许景升, 徐  进, 冯  洁. 一步双重PCR法检测梨火疫病原细菌(Erwinia amylovora). 农业生物技术学报, 待发表.
[15]冯  洁, 徐  进, 许景升, 何礼远. II型分泌系统与植物病原细菌致病性的关系. 农业生物技术学报, 2007, 15: 365-370.
[16]陈长卿, 左豫虎, 康振生. 大豆疫霉菌单游动孢子囊的分离方法. 植物保护, 2006, 32: 106- 107.
[17]左豫虎, 侯巨梅, 康振生, 崔素萍, 黄丽丽. 大豆疫霉菌单孢分离物生物学性状的遗传变异研究. 植物病理学报, 2006, 36: 289-295.
[18]左豫虎, 康振生, 黄丽丽, 韩青梅. 大豆疫霉菌对大豆下胚轴侵染过程的细胞学研究. 植物病理学报, 2005, 35: 235-241.
[19]王化波, 王晓鸣, 朱振东. 中国大豆疫霉菌遗传多样性的RAPD 分析. 菌物系统, 2003, 22: 219-227.
[20]王子迎, 王源超, 张正光, 郑小波. 中国和美国大豆疫霉群体遗传结构的RAPD比较分析. 科学通报, 2006, 51: 1913-1919.
[21]朱振东, 王化波, 王晓鸣, 常汝镇, 武小菲. 中国大豆疫霉菌分布及毒力多样性研究. 中国农业科学, 2003 , 36 : 793-799.
[22]王晓鸣, 朱振东, 王化波, 武小菲. 中国大豆疫霉根腐病和大豆种质抗病性研究. 植物病理学报, 2001, 31: 324-329.
[23]朱振东, 霍云龙, 王晓鸣, 黄俊斌, 武小菲. 一个抗大豆疫霉根腐病新基因的分子鉴定. 作物学报, 2007, 33: 154-157.
[24]张正光, 王源超, 郑小波. 大豆疫霉和苜蓿疫霉rDNA ITS序列分析. 菌物系统, 2003, 22: 542-548.
[25]王立安, 张文利, 王源超, 王良华, 郑小波. 大豆疫霉的ITS 分子检测. 南京农业大学学报, 2004, 27: 38-41.
[26]Wang Y C, Zhang W L, Wang Y, Zheng X B. Rapid and sensitivity detection of Phytophthora sojae in soil and infected soybeans by species-specific polymerase chain reaction assays. Phytopathology, 2006, 96: 1315-1321.
[27]陈长卿, 康振生, 王晓杰, 黄丽丽, 左豫虎. 大豆疫霉的分子检测. 西北农林科技大学学报, 2005, 33: 73-77.
[28]王良华, 丁国云, 王源超, 郑小波. 大豆疫霉菌检测技术研究进展. 植物检疫, 2006, 20: 366-368.
[29]Zhu Z D, Huo Y L, Wang X M, Huang J B, Wu X F. Analysis of simple sequence repeats markers derived from Phytophthora sojae expressed sequence tags. Chinese Science Bulletin, 2004, 49: 2041-2046.
[30]Martin R R. Impacts of molecular diagnostic technologies on plant disease management. Annual Review of Phytopathology, 2000, 38: 207-239.
[31]Schaad N W, Frederick R D, Shaw J, Schneider W L, Hickson R, Petrillo M D, Luster D G. Advances in molecular-based diagnostics in meeting crop biosecurity and phytosanitary issues. Annual Review of Phytopathology, 2003, 41: 305-324.
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 [J]. Scientia Agricultura Sinica, 2009, 42(12): 4403-4410 .
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