Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (6): 1237-1246.doi: 10.3864/j.issn.0578-1752.2013.06.018

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Effect of FSH on the mRNA Expression of Cyclin D1 and Cyclin E1 in the Cultured Immature Boar Sertoli Cell

 ZHANG  Jiao-Jiao, WANG  Yi, ZHANG  Hui-Qiong, SUN  Si, SUN  Yan, WANG  Xian-Zhong, ZHANG  Jia-Hua   

  1. 1.College of Animal Science, Veterinary and Aquaculture, Southwest University/Chongqing Key Laboratory of Forage and Herbivorce, Chongqing 400716
    2.Chongqing Animal Disease Prevention and Control Center, Chongqing 401147
  • Received:2012-09-28 Online:2013-03-15 Published:2012-12-20

Abstract: 【Objective】 The objective of the study is to identify whether or how FSH regulates the mRNA expression of cyclin D1 and cyclin E1 in the cultured immature boar Sertoli cell. 【Method】 In the study, the cultured immature boar Sertoli cells were used as materials, and real-time PCR was used to detect the mRNA expression of cyclin D1 and cyclin E1. 【Result】 Different doses of FSH (0-100 ng•mL-1) increased the mRNA expression of cyclin D1 and cyclin E1 (P<0.05), which were peaking at 50 ng•mL-1 (P<0.05). In addition, FSH (50 ng•mL-1) increased the mRNA expression of cyclin D1 and cyclin E1 in a time-dependent manner (P<0.05) and peaked at 30 min (P<0.05). Different doses of Forskolin (0-20 μmol•L-1) increased the mRNA expression of cyclin D1 and cyclin E1 (P<0.05), but lower compared with FSH alone. Rp-cAMP (0-40 μmol•L-1), H-89 (0-30 μmol•L-1) and Verapamil (0-100 μg•mL-1) inhibited FSH induced the mRNA expression of cyclin D1 and cyclin E1 in a dose-dependent manner (P<0.05), and the combination of Rp-cAMP and Verapamil had more inhibition on FSH activity than individual effect (P<0.05), but lower than addition of Rp-cAMP and Verapamil. Furthermore, different doses of U0126 (0-10 μmol•L-1) reduced FSH induced the mRNA expression of cyclin D1 and cyclin E1 (P<0.05), while Rp-cAMP, H-89, Verapamil or U0126 alone had no effect on the mRNA expression of cyclin D1 and cyclin E1 as compared to the control (P>0.05). 【Conclusion】 FSH regulates the mRNA expression of cyclin D1 and cyclin E1 in a dose- and time-dependent manner, while cAMP-PKA, Ca2+ and extracellular signal-regulated kinase (ERK1/2) is involved in the regulation of FSH on the mRNA expression of cyclin D1 and cyclin E1.

Key words: follicle-stimulating hormone , sertoli cell , cyclin D1 , cyclin E1

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