FSH对体外培养仔猪睾丸支持细胞cyclin D1 mRNA和 cyclin E1 mRNA表达的影响

doi:10.3864/j.issn.0578-1752.2013.06.018

Abstract

Abstract: 【Objective】 The objective of the study is to identify whether or how FSH regulates the mRNA expression of cyclin D1 and cyclin E1 in the cultured immature boar Sertoli cell. 【Method】 In the study, the cultured immature boar Sertoli cells were used as materials, and real-time PCR was used to detect the mRNA expression of cyclin D1 and cyclin E1. 【Result】 Different doses of FSH (0-100 ng•mL-1) increased the mRNA expression of cyclin D1 and cyclin E1 (P＜0.05), which were peaking at 50 ng•mL-1 (P＜0.05). In addition, FSH (50 ng•mL-1) increased the mRNA expression of cyclin D1 and cyclin E1 in a time-dependent manner (P＜0.05) and peaked at 30 min (P＜0.05). Different doses of Forskolin (0-20 μmol•L-1) increased the mRNA expression of cyclin D1 and cyclin E1 (P＜0.05), but lower compared with FSH alone. Rp-cAMP (0-40 μmol•L-1), H-89 (0-30 μmol•L-1) and Verapamil (0-100 μg•mL-1) inhibited FSH induced the mRNA expression of cyclin D1 and cyclin E1 in a dose-dependent manner (P＜0.05), and the combination of Rp-cAMP and Verapamil had more inhibition on FSH activity than individual effect (P＜0.05), but lower than addition of Rp-cAMP and Verapamil. Furthermore, different doses of U0126 (0-10 μmol•L-1) reduced FSH induced the mRNA expression of cyclin D1 and cyclin E1 (P＜0.05), while Rp-cAMP, H-89, Verapamil or U0126 alone had no effect on the mRNA expression of cyclin D1 and cyclin E1 as compared to the control (P＞0.05). 【Conclusion】 FSH regulates the mRNA expression of cyclin D1 and cyclin E1 in a dose- and time-dependent manner, while cAMP-PKA, Ca2+ and extracellular signal-regulated kinase (ERK1/2) is involved in the regulation of FSH on the mRNA expression of cyclin D1 and cyclin E1.

Key words: follicle-stimulating hormone , sertoli cell , cyclin D1 , cyclin E1

ZHANG Jiao-Jiao, WANG Yi, ZHANG Hui-Qiong, SUN Si, SUN Yan, WANG Xian-Zhong, ZHANG Jia-Hua. Effect of FSH on the mRNA Expression of Cyclin D1 and Cyclin E1 in the Cultured Immature Boar Sertoli Cell[J].Scientia Agricultura Sinica, 2013, 46(6): 1237-1246.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2013.06.018

https://www.chinaagrisci.com/EN/Y2013/V46/I6/1237

References

[1]Wood M A, Walker W H. USF1/2 transcription factor DNA-binding activity is induced during rat sertoli cell differentiation. Biology of Reproduction, 2009, 80(1): 24-33.

[2]Franca L R, Silva V A Jr., Chiarini-Garcia H, Garcia S K, Debeljuk L. Cell proliferation and hormonal changes during postnatal development of the testis in the pig. Biology of Reproduction, 2000, 63(6): 1629-1636.

[3]Assoian R K, Yung Y. A reciprocal relationship between Rb and Skp2: implications for restriction point control, signal transduction to the cell cycle and cancer. Cell Cycle, 2008, 7(1): 24-27.

[4]Jiang H, Martin V, Gomez-Manzano C, Johnson D G, Alonso M, White E, Xu J, McDonnell T J, Shinojima N, Fueyo J. The RB-E2F1 pathway regulates autophagy. Molecular and Cellular Pathobiology, 2010, 70(20): 7882-7893.

[5]Bashir T, Pagano M. Don’t skip the G1 phase: how APC/CCdh1 keeps SCFSKP2 in check. Cell Cycle, 2004, 3(7): 850-852.

[6]甘瑞, 左敬, 张国升, 朱峰伟, 孙燕, 王鲜忠, 张家骅. FSH对体外培养仔猪睾丸支持细胞skp2表达的影响. 畜牧兽医学报, 2011, 42(6): 765-771.

Gan R, Zuo J, Zhang G S, Zhu F W, Sun Y, Wang X Z, Zhang J H. The effect of FSH on the expression of skp2 in cultured immature boar sertoli cells. Acta Veterinaria et Zootechnica Sinica, 2011, 42(6): 765-771. (in Chinese)

[7]Wang X Z, Zhao B C, Zhou Y L, Zhou Y T, Ma K G, Zhang J H. 17β-estradiol regulates cultured immature boar sertoli cell proliferation via the cAMP-ERK1/2 pathway and the estrogen receptor β. Agricultural Sciences in China, 2010, 9(8): 1201-1210.

[8]张国升, 左敬, 甘瑞, 朱峰伟, 孙燕, 王鲜忠, 张家骅. FSH和17β-雌二醇联合作用对仔猪睾丸支持细胞Skp2表达的调节. 中国农业科学, 2011, 44(11): 2347-2354.

Zhang G S, Zuo J, Gan R, Zhu F W, Sun Y, Wang X Z, Zhang J H. Effect of combination of FSH and 17β-estradiol on the expression of Skp2 in cultured immature boar sertoli cell. Scientia Agricultura Sinica, 2011, 44(11): 2347-2354. (in Chinese)

[9]Lucas T F, Siu E R, Esteves C A, Monteiro H P, Oliveira C A, Porto C S, Lazari M F M. 17beta-estradiol induces the translocation of the estrogen receptors ESR1 and ESR2 to the cell membrane, MAPK3/1 phosphorylation and proliferation of cultured immature rat sertoli cells. Biology of Reproduction, 2008, 78(1): 101-114.

[10]Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-delta delta C(T) method. Methods, 2001, 25(4): 402-408.

[11]Johnston H, Baker P J, Abel M, Charlton H M, Jackson G, Fleming L, Kumar R K, O’shaughnessy P J. Regulation of Sertoli cell number and activity by follicle-stimulating hormone and androgen during postnatal development in the mouse. Endocrinology, 2004, 145(1): 318-329.

[12]Holsberger D R, Cooke P S. Understanding the role of thyroid hormone in sertoli cell development: a mechanistic hypothesis. Cell and Tissue Research, 2005, 322(1): 133-140.

[13]Godmann M, Kosan C, Behr R. Kruppel-like factor 4 is widely expressed in the mouse male and female reproductive tract and responds as an immediate early gene to activation of the protein kinase A in TM4 sertoli cells. Reproduction, 2010, 139(4): 771-782.

[14]Crepieux P, Marion S, Martinat N, Fafeur V, Vern Y L, Kerboeuf D, Guillou F, Reiter E. The ERK-dependent signalling is stage- specifically modulated by FSH, during primary sertoli cell maturation. Oncogene, 2001, 20(34): 4696-4709.

[15]Dohda T, Maljukova A, Liu L, Heyman M, Grander D, Brodin D, Sangfelt O, Lendahl U. Notch signaling induces SKP2 expression and promotes reduction of p27Kip1 in T-cell acute lymphoblastic leukemia cell lines. Experimental Cell Research, 2007, 313(14): 3141-3152.

[16]Handra-Luca A, Ruhin B, Lesty C, Pierre F. P27, SKP2, and extra-cellular signal-related kinase signalling in human salivary gland mucoepidermoid carcinoma. Oral Oncology, 2006, 42(10): 1005-1010.

[17]Ohtsubo M, Theodoras A M, Schumacher J, Roberts J M, Pagano M. Human cyclin E, a nuclear protein essential for the G1-to-S phase transition. Molecular and Cellular Biology, 1995, 15(5): 2612-2624.

[18]Guo Z Y, Hao X H, Tan F F, Pei X, Shang L M, Jiang X L, Yang F. The elements of human cyclin D1 promoter and regulation involved. Clin Epigenet, 2011, 2(2): 63-76.

[19]El-Kady A, Sun Y, Li Y X, Liao D J. Cyclin D1 inhibits whereas c-Myc enhances the cytotoxicity of cisplatin in mouse pancreatic cancer cells via regulation of several members of the NF-κB and Bcl-2 families. Journal of Carcinogenesis, 2011, 10: 24-37.

[20]Bouskine A, Nebout M, Brucker-Davis F, Benahmed M, Fenichel P. Low doses of bisphenol A promote human seminoma cell proliferation by activating PKA and PKG via a membrane G-protein-coupled estrogen receptor. Environmental Health Perspectives, 2009, 117(7): 1053-1058.

[21]Lalevee N, Rogier C, Becq F, Joffre M. Acute effects of adenosine triphosphates, cyclic3’, 5’-adenosine monophosphates, and follicle-stimulating hormone on cytosolic calcium level in cultured immature rat sertoli cells. Biology of Reproduction, 1999, 61(2): 343-352.

[22]Kahl C R, Means A R. Calcineurin regulates cyclin D1 accumulation in growth-stimulated fibroblasts. Molecular Biology of the Cell, 2004, 15(4): 1833-1842.

[23]Choi J, Chiang A, Taulier N, Gros R, Pirani A, Husain M. A calmodulin-binding site on cyclin E mediates Ca2+-sensitive G1/S transitions in vascular smooth muscle cells. Cirulation Research, 2006, 98(10): 1273-1281.

[24]Nakayama K I, Hatakeyama S, Nakayama K. Regulation of the cell cycle at the G1-S transition by proteolysis of cyclin E and p27Kip1. Biochemical and Biophysical Research Communications, 2001, 282(4): 853-860.

[25]Hoshino R, Tanimura S, Watanabe K, Kataoka T, Kohno M. Blockade of the extracellular signal-regulated kinase pathway induces marked G1 cell cycle arrest and apoptosis in tumor cells in which the pathway is constitutively activated. The Journal of Biological Chemistry, 2001, 276(4): 2686-2692.

[26]Cha H, Shapiro P. Tyrosine-phosphorylated extracellular signal– regulated kinase associates with the Golgi complex during G2/M phase of the cell cycle: evidence for regulation of golgi structure. The Journal of Cell Biology, 2001, 153(7): 1355-1367.

[27]王鲜忠, 周玉兰, 赵伯川, 周银涛, 陈永军, 张姣姣, 王怡, 张家骅. FSH通过cAMP、Ca2+调节仔猪睾丸支持细胞的增殖. 畜牧兽医学报, 2010, 41(10): 1240-1245.

Wang X Z, Zhou Y L, Zhao B C, Zhou Y T, Chen Y J, Zhang J J, Wang Y, Zhang J H. FSH regulates boar sertoli cell proliferation via cAMP and Ca2+ influx. Acta Veterinaria et Zootechnica Sinica, 2010, 41(10): 1240-1245. (in Chinese)

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Effect of FSH on the mRNA Expression of Cyclin D1 and Cyclin E1 in the Cultured Immature Boar Sertoli Cell

PDF

Cited

Abstract

Cite this article

share this article

References

Related Articles 3

Metrics

Comments

Recommended 0

[1]	. Distribution of FSH Receptor on the Ovaries Among Different Sheep Breeds by Different Efficiency of Superovulation [J]. Scientia Agricultura Sinica, 2011, 44(6): 1232-1238 .
[2]	ZHANG Guo-sheng,ZUO Jing,GAN Rui,ZHU Feng-wei,SUN Yan,WANG Xian-zhong,ZHANG Jia-hua. Effect of Combination of FSH and 17β-estradiol on the Expression of Skp2 in Cultured Immature Boar Sertoli Cell [J]. Scientia Agricultura Sinica, 2011, 44(11): 2347-2354 .
[3]	ZUO Jing, GAN Rui, ZHANG Guo-Sheng, ZHANG Jiao-Jiao, ZHU Feng-Wei, SUN Yan, WANG Xian-Zhong, ZHANG Jia-Hua. 17beta-Estradiol Regulates the Expression of CyclinA2 mRNA of Cultured Immature Boar Sertoli Cells via Estrogen Receptor beta, cAMP-PKA and ERK1/2 [J]. Scientia Agricultura Sinica, 2011, 44(10): 2139-2145.