Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (14): 2959-2966.doi: 10.3864/j.issn.0578-1752.2012.14.019

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Effects of Dietary Supplementation with Hainanmycin or Monensin on Ruminal Protein Degradation and Populations of Ammonia-Producing Bacteria in Dairy Cows

 WANG  Zhi-Bo, XIN  Hang-Shu, DUAN  Chun-Yu, YAO  Qing, ZHAO  Hong-Bo, ZHANG  Yong-Gen   

  1. 1.东北农业大学动物科学与技术学院,哈尔滨 150030
    2.黑龙江八一农垦大学动物科技学院,黑龙江大庆 163319
  • Received:2011-11-02 Online:2012-07-15 Published:2011-12-31

Abstract: 【Objective】The study was conducted to evaluate the effects of dietary supplementation with hainanmycin or monensin on ruminal protein degradation and populations of ammonia-producing bacteria.【Method】Three ruminally cannulated cows were randomly assigned to treatments in a 3×3 Latin square design with a 12-d period. The three dietary treatments were: a control diet, control diet plus supplemental Hainanmycin at 75 mg?d-1, and control diet plus supplemental monensin at 150 mg?d-1.【Result】The results showed that ruminal relative population sizes of Prevotella ruminicola of cows supplemented with hainanmycin or monensin were higher than the control significantly (P<0.05), and that of Butyrivibrio fibrisolvens and ammonia-hyperproducing bacteria were lower than the control significantly (P<0.05). However, ionophore had no effect on relative population sizes of Megasphaera elsdenii, Selenomonas ruminantium and Streptococcus bovis (P>0.05). Compared with the control, hainanmycin or monensin supplementation increased peptide nitrogen and amino acid nitrogen concentration (P<0.05) and reduced ammonia concentration and deaminase activity (P<0.05), and had no effect on proteinase activity (P>0.05). There were no difference between hainanmycin and monensin (P>0.05).【Conclusion】Similarly to monensin, hainanmycin had the protein-sparing effect, and could modify degradation of protein by changing specified ammonia-producing bacteria.

Key words: hainanmycin, monensin, protein degradation, ammonia-producing bacteria

[1]Callaway T R, Edrington T S, Rychlik J L, Genovese K J, Poole T L, Jung Y S, Bischoff K M, Anderson R C, Nisbet D J. Ionophores: their use as ruminant growth promotants and impact on food safety. Current Issues in Intestinal Microbiology, 2003, 4: 43-51.

[2]Chen G J, Russell J B. Effect of monensin and a protonophore on protein degradation, peptide accumulation, and deamination by mixed ruminal microorganisms in vitro. Journal of Animal Science, 1991, 69(5): 2196-2203.

[3]Uwituze S, Parsons G L, Karges K K, Gibson M L, Hollis L C, Higgins J J, Drouillard J S. Effects of distillers grains with high sulfur concentration on ruminal fermentation and digestibility of finishing diets. Journal of Animal Science, 2011, 89(9): 2817-2828.

[4]Yang C M, Russell J B. Effect of monensin on the specific activity of ammonia production by ruminal bacteria and disappearance of amino nitrogen from the rumen. Applied and Environmental Microbiology, 1993, 59(10): 3250-3254.

[5]Krause D O, Russell J B. An rRNA approach for assessing the role of obligate amino acid-fermenting bacteria in ruminal amino acid deamination. Applied and Environmental Microbiology, 1996, 62(3): 815–821.

[6]黄  兵. 离子载体抗球虫新药-海南霉素. 养禽与禽病防治, 2002(5): 16-17.

Huang B. New antibiotic of resisting coccidia-Hainanmycin. Poultry Husbandry and Disease Control, 2002(5): 16-17. (in Chinese)

[7]Ren M Q, Shen Z M, Zhao R Q, Lu T S, Chen J. Effects of novel polyether ionophore hainanmycin on nutrient digestion, metabolism and ruminal characteristics of goats. Journal of Animal and Feed Sciences, 1998, 7: 21-28.

[8]沈赞明, 陈  杰, 任明强. 海南霉素对山羊瘤胃内肽的保护作用. 南京农业大学学报, 2004, 27(4): 73-76.

Shen Z M, Chen J, Ren M Q. Protective effects of hainanmysin on concentration of rumen peptides in goats. Journal of Nanjing Agricultural University, 2004, 27(4): 73-76. (in Chinese)

[9]吕爱军, 穆阿丽, 宋小敬, 张关东. 聚醚类抗生素的作用机理及应用研究进展. 吉林畜牧兽医, 2004(5): 22-24.

Lü A J, Muali, Song X J, Zhang G D. Polyether antibiotics application study progress in animal husbandry production. Jilin Animal Science and Veterinary Medicine, 2004(5): 22-24. (in Chinese)

[10]National Research Council. Nutrient Requirements of Dairy Cattle. 7th ed. Washington: National Academic Press, 2001, 252-308.

[11]Bürgmann H. A strategy for optimizing quality and quantity of DNA extracted from soil. Journal Microbiological Methods, 2001, 45(1): 7-20.

[12]Stevenson D M, Weimer P J. Dominance of Prevotella and low abundance of classical ruminal bacterial species in the bovine rumen revealed by relative quantification real-time PCR. Applied Microbiology and Biotechnology, 2007, 75(1): 165-174.

[13]Wessels R H, Titgemeyer E C, Armendariz C K, Jean G S. Lasalocid effects on ruminal degradation of protein and postruminal supply of amino acids in Holstein steers. Journal of Dairy Science, 1996, 79(10): 1802-1808.

[14]Siddons R G, Paradine J. Effect of diet on protein degrading activity in the sheep rumen. Journal of the Science of Food and Agriculture, 1981, 32(10): 973-981.

[15]Weimer P J, Stevenson D M, Mertens D R, Thomas E E. Effect of monensin feeding and withdrawal on populations of individual bacterial species in the rumen of lactating dairy cows fed high-starch rations. Applied Microbiology and Biotechnology, 2008, 80(1): 135-145.

[16]Chen M, Wolin M J. Effect of monensin and lasalocid-sodium on the growth of methanogenic and rumen saccharolytic bacteria. Applied and Environmental Microbiology, 1979, 38(1): 72-77.

[17]Russell J B, Strobel H J. Effect of ionophores on ruminal fermentation. Applied and Environmental Microbiology, 1989, 55(1): 1-6.

[18]Newbold CJ, Wallace R J, Walker N D. The effect of tetronasin and monensin on fermentation, microbial numbers and the development of ionophoreresistant bacteria in the rumen. Journal of Applied Bacteriology, 1993, 75(2): 129-134.

[19]Callaway T R, Adams K A, Russell J B. The ability of “low G + C gram-positive” ruminal bacteria to resist monensin and counteract potassium depletion. Current Microbiology, 1999, 39: 226-230.

[20]Wallace R J, Onodera R, Cotta M A. Metabolism of Nitrogencontaining Compounds. In the Rumen Microbial Ecosystem. London: Chapman and Hall, 1997.

[21]Chen G, Russell J B. More monensin-sensitive, ammonia-producing bacteria from the rumen. Applied and Environmental Microbiology, 1989, 55(5): 1052-1057.

[22]Callaway T R, de Melo A M S C, Russell J B. The effect of nisin and monensin on ruminal fermentations in vitro. Current Microbiology, 1997, 35(2): 90-96.

[23]Yang C M, Russell J B. The effect of monensin supplementation on ruminal ammonia accumulation in vivo and the numbers of amino acid-fermenting bacteria. Journal of Animal Science, 1993, 71(12): 3470-3476.

[24]Wallace R J. Ruminal microbial metabolism of peptides and amino acids. Journal of Nutrition, 1996, 126(Suppl.): 1326-1334.

[25]Armstead I P, Ling J R. Variations in the uptake and metabolism of peptides and amino acids by mixed ruminal bacteria in vitro. Applied and Environmental Microbiology, 1993, 59(10): 3360-3366.

[26]Newbold C J, Wallace R J, Watt N D. Properties of ionophore-resistant Bacteroides ruminicola enriched by cultivation in the presence of tetronasin. Journal of Applied Bacteriology, 1992, 72(1): 65-70.

[27]Russell J B, O’Connor J D, Fox D G, van Soest P J, Sniffen C J. A net carbohydrate and protein system for evaluating cattle diets: I. ruminal fermentation. Journal of Animal Science, 1992, 70: 3551-3561.

[28]李炯明, 庄  苏, 王  恬, 丁立人. 海南霉素对人工瘤胃体外发酵调控的影响. 家畜生态学, 2007, 28(1): 41-46.

Li J M, Zhuang S, Wang T, Ding L R. The effects of hainanmycin on artificial rumen fermentation in vitro. Journal of Domestic Animal Ecology, 2007, 28(1): 41-46. (in Chinese)

[29]Ives S E, Titgemeyer E C, Nagaraja T G, del Barrio A, Bindel D J, Hollis L C. Effects of virginiamycin and monensin plus tylosin on ruminal protein metabolism in steers fed corn-based finishing diets with or without wet corn gluten feed. Journal of Animal Science, 2002, 80(11): 3005-3015.

[30]Bergen W G, Bates D B. Ionophores: their effect on production efficiency and mode of action. Journal of Animal Science, 1984, 58(6): 1465-1483.

[31]Wallace R I, Cotta M A. Metabolism of Nitrogen-Containing Compounds//The Rumen Microbial Ecosystem. London: Elsevier Applied Science Publishers, 1988.

[32]Newbold C J, Wallace R J, McKain N. Effects of the ionophore tetronasin on nitrogen metabolism by ruminal microorganisms in vitro. Journal of Animal Science, 1990, 68(4): 1103-1109.
[1] CHEN Li-juan, LI Xin, LI Zheng, LI Pei-di, LI Zhong-wen, ZHANG De-quan. Protein Phosphorylation on the Function of Myofibrillar Proteins in Mutton Muscle [J]. Scientia Agricultura Sinica, 2016, 49(7): 1360-1370.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!