Abstract:

【Objective】 Molecular markers were used to identify orchardgrass hybrids and analyze genetic diversity for forming orchardgrass hybrid identification system, detecting genetic information and conservation and utilization of orchardgrass germplasm resources. 【Method】 The sequence-related amplified polymorphism (SRAP) were assessed to analyze 140 orchardgrass hybrid individuals and their parental lines (01996, YA02-103) for detecting true or false hybrids according to polymorphism, and the genetic variation among hybrid individuals were detected using molecular markers and morphological markers. 【Result】 Sixty-four pairs of primers that generated clear, reproducible and polymorphic bands were selected from 192 pairs of SRAP primers with an average of 33.33%. 106 progenies with the special male bands compared with the female bands were identified from 140 progenies, which were therefore recognized as true hybrids. Sixteen primer pairs produced a total of 151 bands, of which 71 bands were polymorphic bands and the average polymorphic bands was four per primer pair. The average percentage of polymorphic bands was 47.24%. 【Conclusion】 These results suggested that SRAP technique has wide prospective in hybrid identification and diversity analysis of orchardgrass.

Key words: orchardgrass (Dactylis glomerata L.), SRAP markers, hybrid identification, genetic diversity analysis