Abstract: 【Objective】The taxonomy and species identification of Trichinella spiralis has been received attention by many scholars. The authors try to determine the different Trichinella spiralis isolates through molecular genetic mechanism and be the base of the traditional taxonomy. The gene segment in 18S ribosome RNA gene of four Trichinella isolates were cloned and sequenced. 【Result】The sequence was compared and analysed by using DNAsis software, and the results showed that the homology of 18SrRNA gene of HH and T. spiralis was 99.4%, that of HC and Trichinella nativa was 99.3%. The Swine isolate belong to T. spiralis, the Dog isolate belongs to T. nativa. The result was consistent with the traditional taxonomy. 【Conclution】The result has provided a strong evidence for the traditionary taxonomy and be the base of the epidemiology and precention to Trichinellosis.

Key words: Trichinella isolates, 18S rRNA, Taxonomy