Scientia Agricultura Sinica ›› 2005, Vol. 38 ›› Issue (08): 1570-1573 .

• PLANT PROTECTION • Previous Articles     Next Articles

Preparation, Characterization and Application of Anti-harpinxoo Antibody

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  • Received:2005-02-04 Revised:1900-01-01 Online:2005-08-10 Published:2005-08-10

Abstract: Polyclonal anti-Harpin rabbit antibody has been successfully prepared by using purified harpin protein as immunogen. The ELISA titer of antiserum against harpin was about 1:2000. Western blot analysis showed that the antiserum could bind to the expression harpin protein specifically. Hrf1 gene, coding harpin, expression in transgenic rice detected by anti-Harpin rabbit antibody. The rabbit antibody against harpin can be used to study the biological function, harpin localization and hrf1 gene expression in other plants further.

Key words: harpin, polyclonal antibody, western blot, expression analysis in transgenic plants

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